...multiple cell types of the same lineage. Blood (hematopoietic) stem cells are a good example of multipotent...numbers or replenish dying cells. For example, hematopoietic stem cells found in the bone marrow only differentiate...three germ layers, form embryoid bodies, and form teratomas [11]. iPSCs also possess similar morphologies,...

...important supporting roles. Enhancers are cis regulatory DNA sequences that, when bound by transcription...CAPTURE (CRISPR Affinity Purification in situ of Regulatory Elements) method. The original CAPTURE method...2020). CRISPR Affinity Purification in situ of Regulatory Elements: The original CAPTURE method The original...

...air conditioning on) so leave them in a sterile incubator. This also means that any medium or washing buffer...entering the hood and when putting them into the incubator). It also helps to get familiar with the morphology...results. Most cell culture media contain the pH indicator phenol red (pH range 6.0 (yellow) - 8.0 (red))...

...localization, dynamics, and behavior of a POI. As an investigator, you want a fusion tag to be inert, to not produce...has gone into making FPs monomeric, yet many investigators remain ignorant of FP dimerization. Equally ...Einstein College of Medicine in the Department of Anatomy and Structural Biology. His interests include the...

...  Is this department/laboratory collaborative with other departments/laboratories? How long is the average...by meeting with professors - a/k/a Principle Investigators (PIs) - whose labs does research that aligns...

...expressing eGFP under the human ubiquitin C promoter. Anatomical abbreviations: Gr= Gracile tract, Cu= Cuneate...  The first successful attempt to render fixed anatomical preparations transparent was done by Walter Spalteholtz...protein (GFP) (6).   The Dodt and Frank Bradke laboratories later developed the 3DISCO (3-dimensional imaging...

...genetically encoded calcium indicators (e.g. GCaMP). Others include: pHluorin & pHTomato (pH sensors), HyPer ...fluorescent intensity upon pH changes (e.g. pHluorin, pHTomato). Keep this list handy as you plan your next ...

...available by both the Jacob Corn and Alex Schier laboratories. Delivering Cas9-gRNA ribonucleoproteins  An...and purification, which will require additional laboratory reagents, equipment, and expertise. Final thoughts...production of the Cas9 protein and a gRNA in your laboratory, once these have been acquired, genomic editing...

...you possess because any potential employers, collaborators, or co-workers want to know that you are a well-rounded...situation? Maybe you demonstrated empathy for a collaborator or co-worker and showed that you really understood...Resources Cross team communication  External collaborators For further advice on highlighting your transferable...

...pictures (see figure 4). It also enables single-cell anatomical study and neuronal tracing, aka determining where...under the control of an inducer, i.e. tet-off transactivator (tTA), is injected at a high dose to maximize...An AAV expressing the inducer (i.e. tet-off transactivator) is co-injected. The dose of this AAV can be...

...do both, Addgene depositor Didier Trono and collaborators turned to the lentivirus HIV-1, well-known to...precursor protein), Pol (polymerase), Tat (viral transactivator for transcriptional activation from the 5’ ...trials for other diseases, including the common hematopoietic disorders sickle cell anemia and beta-thalassemia...

...communication should be fun and engaging for the communicator as well as the audience, so pick a mode of communication...Otherwise, you’re going to drop your project like a hot potato or burn yourself out trying to slog through unappealing... Low barrier to entry for both audience and communicators. Well-developed content creation platforms.  ...

...and debris. Affinity chromatography is a popular choice for this. Chromatography columns containing appropriate...using a desalting column or an ultrafiltration concentrator. As the name suggests, you may also concentrate...

...anxiety by modulating the production of the pro-inflammatory cytokine interleukin IL-1 beta in the dorsal...shRNA to downregulate expression of miR124a, a regulatory RNA that negatively regulates BDNF levels. They...showed that the activity of a particular subset of excitatory neurons (Drd1) in mPFC is responsible for the...

...splicing. (RNAi, in contrast, engages existing gene regulatory mechanisms to block activity and does not work...phiC31. The popular UAS/Gal4 and Cre/Lox gene regulatory systems have also been successfully implemented...Patrick Müller’s group at the FriedrichMiescher Laboratory of the Max Planck Society in Tübingen, Germany...

...feasible, it leaves little room for additional regulatory elements. Feng Zhang's group previously packaged...for Cas9. However, to allow for more room for regulatory sequences while still fitting Cas9 and its gRNAs... https://doi.org/10.1038/nature14299  Senís E, Fatouros C, Große S, Wiedtke E, Niopek D, Mueller A-K, ...

...but not all. For example, LDLR expression in hematopoietic stem cells, T cells, and B cells is low. Infection...great at infecting neurons and cause minimal inflammatory response, but are not taken up by axon terminals...Precious Tools to Improve Gene Modification of Hematopoietic Cells for Research and Gene Therapy. Viruses...

...exhibit slow head growth, develop seizures, cardio-respiratory dysfunction, distinctive hand movements, and ...& Bird, 2019) and is a global transcriptional regulator of thousands of genes. Studies have suggested ...). Beam Therapeutics, co-founded by Harvard Investigators and Addgene depositors David Liu, PhD, Keith...

...tools and assumptions to reveal findings that the originator was oblivious to, no matter how much time they...they had. It could be argued that data generators may, in light of the policy, hold onto data longer before... available to everyone at once, including the generators. This practice doesn’t appear to harm the originating...

...Rhamnose induction relies on the transcriptional activator rhaS. To allow manipulation of bacteria without...synthetic ribosome binding site (using the online calculator from the Salis group) (Espah Borujeni et al.,...mix. Introduction of the strong transcriptional terminator BBa_B1002 (from IGEM) upstream of the MCS to ...