...protein of interest (POI), that facilitate immunohistochemistry (IHC) experiments with tag-specific antibodies...technologies such as array tomography, super-resolution STORM imaging, and electron microscopy. Sample preparation...standard proteins tags like GFP. Specifically for STORM, the high intensity signal provided by smFPs can...Rizzo, Mark A., Michael W. Davidson, and David W. Piston. "Fluorescent protein tracking and detection: fluorescent...

...interactions in living cells using a microscope. Historically, molecular interactions were detected by indirect...brightness of a fluorophore pair, donor:acceptor stoichiometry, and cross-talk between the two fluorophore ...has five-fold less brightness than YFP. The stoichiometry of donor:acceptor is difficult to address especially... Transfer    Interesting Articles About FRET: Piston DW, Kremers GJ. Fluorescent protein FRET: the good...

...diminished KLF4 expression levels; this altered stoichiometry impacted reprogramming and global gene expression...redshifted-emission spectrum compared to that of CyOFP1, a large stokes shift, long fluorescence lifetime, and a stable...affecting many of the RFPs developed from natural ancestors), and screened through libraries of new RFP variants... an FP’s properties. pBAD-LSSmCherry1 is a long Stokes shift variant, which could be compatible with two-photon...entire protein-coding regions (excluding start and stop codons) with kanamycin cassettes and were carefully...

...like rotting eggs but has a longer shelf life when stored properly. Because BME is not as strong as DTT, ..., while expensive, reduce protocol time, can be stored at 4 °C, and are very consistent gel-to-gel.  Ladder...multiple times. It doesn’t degrade during long-term storage and has both high binding capacity and high protein...inexpensive and easily made in the lab. They can be stored at your bench during the procedure and at 4 °C ...Fluorescent proteins are stable, so blots can be stored for weeks to months without loss of signal. While...

...key to fitting large genes into AAV First, some history. In 2008, the Auricchio lab decided to solve the..., the partial genomes complement each other to restore the full-expression cassette. AAV’s natural ability...truncated protein. This can be avoided by excluding a stop codon from donor A in any of the reading frames ...the gRNA target site so that transcripts undergo nonstop decay (Frischmeyer et al., 2002). How to pick ...

...multiple exons that, when deleted through NHEJ, will restore the dystrophin reading frame. gRNAs bind in intronic... blot, at <10% of the wild-type level. Muscle histology is improved, with vastly reduced inflammation ...this result, Nelson et al. find that dystrophin restoration is maintained for at least six months. Future..., Olson EN. Postnatal genome editing partially restores dystrophin expression in a mouse model of muscular...

... translation-blocking STOP cassette immediately following the promoter. The STOP cassette includes a mutant...OFP (orange), is expressed when Cre is absent; stochastic Cre excision results in one of three recombinations...anemone mKate2.) They then successfully generated custom antibodies to each of these proteins and confirmed...

...Stephen M., et al. “Controls for Immunohistochemistry.” Journal of Histochemistry & Cytochemistry, vol. ...Controls for Immunocytochemistry.” Journal of Histochemistry & Cytochemistry, vol. 59, no. 1, SAGE Publications...and Practicalities of Immunoblotting and Immunohistochemistry: A Guide for Submission to the British Journal...

...MasScience, ECUSA, IMFAHE and Bostonadas. Follow her on twitter @Bostonadas.         Rocío López-Diego MD...relationship for the mentee, where (s)he feels truly understood and supported, and can more fully identify with...University, Massachusetts Institute of Technology, Boston University, Johns Hopkins University, the Salk ...

...Cell lines and reagents Examine your cell line stock for mycoplasma contamination before use. Make sure...using expired products or reagents that have been stored for a longer time. Use fresh media as much as possible...for prevention. Prepare a small quantity of media stock that would be used for the experiment, preventing...line to another. Multiple cell lines should not be stored in a single incubator since it increases the risk...

...undergraduate years. We each have a unique “origin story,” but taking the first step into the world of academic... to find out what a “postdoc” is, and that “PI” stood for principal investigator. In basic science (as...after earning a doctorate Considered a "stepping stone" position to become a professor, or other scientific...the value in finding mentors wherever I go. This story by Dr. Erika Moore in Science Careers discusses ...

...into inserting a fluorophore-tagged amino acid at a stop codon (often amber i.e. UAG) engineered into a desired...suppression competes with natural chain termination at stop codons, expression levels of these tagged proteins...Research department at Brigham and Women’s Hospital in Boston, MA. His interests include developing novel fluorescent...British anti-Lewisite (dimercaprol): an amazing history." Annals of emergency medicine 41.3 (2003): 378...

...application, and in such cases, it may be necessary to customize an existing reagent to suit your needs. Genetic...

...in grad school was that scientists have amazing stories to share. I spend a lot of time telling scientists...for genome editing? Know a professor with a great story to tell? Let us know by emailing us at [email protected] on Twitter and Facebook. We’ll track down the story, get the inside scoop, and keep you informed.   ...

...cellulosic biomass to useful products under Professor Christopher E. French. Dr. Duedu was recently awarded plasmids...plasmid grant. 7:12 - 7:11 : Kwabena's work in Christopher E. French's Lab at the University of Edinburgh...opportunities in Ghana. 46:35 - end : Kwabena's time in Boston - Developing microbiome expertise. Many thanks...

... online access on scientific impact Partly, the story has to do with online accessibility. It is now about... recently reported that the most cited work in history is a 1951 description of an assay to determine ...of scientific publications. Ventures like Impact Story seek to find ways to capture scholarly work that...

...Research) – and an Addgene depositing scientist – stop by our booth to say hi! Zhang was awarded one of... to help their labs save time and resources by storing and distributing their plasmids with the scientific...our booth, I will have more updates coming soon. Stop by booth #3431 if you’re at #SfN14 and meet our ...

...endeavor October 2-3, 2014 Boston University Jacob Sleeper Auditorium, Boston, MA Register on Eventbrite...reading this article - you should join Dr. Bourne in Boston on October 2nd when he will be the keynote speaker...

... entered Feng Zhang’s lab at MIT, the use of customizable DNA-binding domains based on transcription-activator-like...expression via epigenetics with the addition of histone modifiers. “We want to allow people to prove the...476 (22 August 2013).   , with the addition of histone modifiers, ...

... By Walking Around" – don't hide in your office. Stop by instead of emailing.    Demonstrate you are ...clarify the situation; C = confirm that you have understood the speaker accurately. Thank employees who volunteer...by feedback. If you don't do this, employees will stop providing feedback pretty quickly.   This is the...