Live and Let Dye: Self-Labeling Protein Tags
Type
Blog Post
... chemistry. Although these multi-component systems can be complicated to implement, they can be highly...Various
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Complicated
Non-natural amino acids
1
Various
Many
Complicated
Applications... outer mitochondrial membrane labeled with SNAP-OMP25 with Atto590-BG (green), imaged with stimulated ...STED) nanoscopy. Scale bar, 2 μm. Inset shows comparison with standard confocal microscopy. Image adapted... assembly-regulating proteins in vitro reveals complex events. a) Micrographs of a fluorescently-labeled...159 aa) are smaller than HaloTag (295 aa) – for comparison, common fluorescent proteins are typically 230...Wissner, R. F., Xi, Z., Allgeyer, E. S., Zhong, S., Thompson, A. D., Lowe, N., Butler, R., Bewersdorf, J., ...