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We narrowed to 612 results for: GAN

Showing: 221 - 240 of 612 results

  1. CRISPR in the Clinic

    Type
    Blog Post
    ...functional correction of the gene in clonally expanded organoids. This work, and many others, pushed the field ... post was originally written in 2014 by Kendall Morgan and updated in 2023 by Susanna Stroik.  References...of CFTR by CRISPR/Cas9 in Intestinal Stem Cell Organoids of Cystic Fibrosis Patients." Cell Stem Cell. ...

  2. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...concentrations and transients in living cells and organisms. Unlike chemical indicators, these DNA constructs...constructs can be delivered to intact tissues and organs noninvasively and target a defined population of cells...Genetically encoded fluorescent indicators for organellar calcium imaging. Biophysical journal. 2016 Sep...

  6. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...of NHEJ, HDR, and MMEJ repair varies from organism to organism. MMEJ doesn’t yield a “perfect repair” like...researchers can exploit for CRISPR gene editing. In organisms where HDR is downregulated, MMEJ represents another...

  8. E11 Bio PRISM Collection

    Type
    Collection
    ... new window) , a non-profit focused research organization supported by Convergent Research (Link opens...

  9. Synthetic Biology - Overview

    Type
    Collection
    ...plasmid-strain functional pairs. Browse Plasmids by Model Organism Bacterial Plant Yeast Mammalian Worm Fungal Algal...

  10. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...slices, which are easier to expand than, say, a C. elegans (Damstra, et al. 2022; Yu, et al. 2020). With any...//doi.org/10.7554/elife.73775  Dani A, Huang B, Bergan J, et al (2010) Superresolution Imaging of Chemical...Wassie AT, et al (2020) Expansion microscopy of C. elegans. Elife 9:e46249. https://doi.org/10.7554/elife.46249...

  11. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...Chromatography columns containing appropriate immobilized ligands are incubated with cell culture supernatant containing...antibodies. The antibodies bind the immobilized ligands and can be eluted once the other materials have...incubated with the cell suspension. Antibodies bind ligands on the beads and a magnet is used to separate the...

  12. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...generated using random genomic fragments from the organism of interest, or a defined cDNA library (e.g. the...number of two-hybrid systems available in model organisms, including bacteria, alternative fungi (C. albicans...Enzymol. 1983;100:293-308. PubMed PMID: 6312261. 2. Keegan, L., Gill, G., and Ptashne, M. Separation of DNA...

  13. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...(CNS) orchestrates complex processes enabling organisms to control their movements and behavior. These...to study connectomics (how neural circuits are organized and how neurons connect to each other). Combined.... 2. Shepherd, G.M. (2004). In "The synaptic organization of the brain". Oxford University Press, New ...

  14. How to Write a Scientific Review Article

    Type
    Blog Post
    ...one either. Choose the topic and outline the organization of the review Once you start reading, there ...references) and it’s better to keep your references organized from the beginning. I also recommend using the...Manuscripts: Literature Searching, Reading, & Organizing Tips for Improving Your Next Manuscript 6 Tips...

  15. Lab to Office Culture Shock

    Type
    Blog Post
    ... number of career paths--industry, nonprofit organization, law office, journal editor, etc.--so everyone...around the world, such as BosLab here in Boston. Organizations like this one are always looking for scientists... own independent research!). There are also organizations like Science from Scientists, which try to bring...

  16. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

    Type
    Blog Post
    ...partnerships with like-minded individuals and organizations, like the Allen Institute, to achieve greater...together. The Allen Institute is a research organization that is committed to understanding some of the...reliable tools to help carry out that research. Organizations like the Allen Institute are committed to just...

  17. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ... that the drug prevents the growth of a living organism. As for multi-drug resistance: definitions vary...vary in the literature, but generally organisms with multi-drug resistance are resistant to three or more...is an intense amount of competition between microorganisms for available resources. This competition drives...

  18. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...over-express coding sequences from a given cell type or organism, have been used in a similar manner to Cas9 activators...lines (HEK293T, MCF7, U2-OS, Hela, N2A, 3T3) and organisms (Chavez et al., 2016). To learn more about the...Tuttle M, P R Iyer E, Lin S, Kiani S, Guzman CD, Wiegand DJ, Ter-Ovanesyan D, Braff JL, Davidsohn N, Housden...

  19. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    ...distinct subcellular environments, including the organelles that compose the secretory pathway, endocytic...formation, and proteolytic cleavage. FPs localized to organelles are equally susceptible to these non-native modifications... Lee Snapp. "Fluorescent proteins in cellular organelles: serious pitfalls and some solutions." DNA and...

  20. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    ...non-human primates [7, 8], zebrafish [9], and c. elegans [10]. If used properly, optogenetics is sure to...Cellular and Molecular Biology from the University of Michigan and he is currently a Postdoctoral Fellow in the...neural activity in freely moving Caenorhabditis elegans." Nature methods 8.2 (2011): 147-152. PubMed PMID...
Showing: 221 - 240 of 612 results