We narrowed to 612 results for: GAN

...functional correction of the gene in clonally expanded organoids. This work, and many others, pushed the field ... post was originally written in 2014 by Kendall Morgan and updated in 2023 by Susanna Stroik.  References...of CFTR by CRISPR/Cas9 in Intestinal Stem Cell Organoids of Cystic Fibrosis Patients." Cell Stem Cell. ...

...concentrations and transients in living cells and organisms. Unlike chemical indicators, these DNA constructs...constructs can be delivered to intact tissues and organs noninvasively and target a defined population of cells...Genetically encoded fluorescent indicators for organellar calcium imaging. Biophysical journal. 2016 Sep...

...tech transfer team will communicate with your organization’s technology transfer office or other authorized.... rerio (zebrafish), D. melanogaster (fly), C. elegans (nematode), S. cerevisiae (budding yeast), S. pombe.... The name of the Principal Investigator and Organization where your plasmids were first created. From...

...CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids page . Find a ...

...to deliver a standard AdV vector to the targeted organs or tissues with Cas9 and a gRNA designed to target...67 Ding Q, Strong A, Patel KM, Ng S-L, Gosis BS, Regan SN, Cowan CA, Rader DJ, Musunuru K (2014) Permanent...  Kendall Morgan...

...in 720°, i.e. they are on a membrane or in an organelle, then their effective concentration will be much...the membranes into stacked structures including organized smooth endoplasmic reticulum (OSER) whorls. 2)...distorted into dense stacked membranes termed Organized Smooth Endoplasmic Reticulum or OSER if a dimerizing...

...of NHEJ, HDR, and MMEJ repair varies from organism to organism. MMEJ doesn’t yield a “perfect repair” like...researchers can exploit for CRISPR gene editing. In organisms where HDR is downregulated, MMEJ represents another...

...a co-founder of the BioBricks Foundation, an organization working to advance biological engineering openly... technical challenge. We have a strategy that organizes how we start the work, and it begins with the ... Kendall Morgan...

...bar or sort buttons to find plasmids based on: Organism or species (e.g., human, watermelon, Streptomyces...

...Chromatography columns containing appropriate immobilized ligands are incubated with cell culture supernatant containing...antibodies. The antibodies bind the immobilized ligands and can be eluted once the other materials have...incubated with the cell suspension. Antibodies bind ligands on the beads and a magnet is used to separate the...

...slices, which are easier to expand than, say, a C. elegans (Damstra, et al. 2022; Yu, et al. 2020). With any...//doi.org/10.7554/elife.73775  Dani A, Huang B, Bergan J, et al (2010) Superresolution Imaging of Chemical...Wassie AT, et al (2020) Expansion microscopy of C. elegans. Elife 9:e46249. https://doi.org/10.7554/elife.46249...

...generated using random genomic fragments from the organism of interest, or a defined cDNA library (e.g. the...number of two-hybrid systems available in model organisms, including bacteria, alternative fungi (C. albicans...Enzymol. 1983;100:293-308. PubMed PMID: 6312261. 2. Keegan, L., Gill, G., and Ptashne, M. Separation of DNA...

... new window) , a non-profit focused research organization supported by Convergent Research (Link opens...

...partnerships with like-minded individuals and organizations, like the Allen Institute, to achieve greater...together. The Allen Institute is a research organization that is committed to understanding some of the...reliable tools to help carry out that research. Organizations like the Allen Institute are committed to just...

...one either. Choose the topic and outline the organization of the review Once you start reading, there ...references) and it’s better to keep your references organized from the beginning. I also recommend using the...Manuscripts: Literature Searching, Reading, & Organizing Tips for Improving Your Next Manuscript 6 Tips...

... number of career paths--industry, nonprofit organization, law office, journal editor, etc.--so everyone...around the world, such as BosLab here in Boston. Organizations like this one are always looking for scientists... own independent research!). There are also organizations like Science from Scientists, which try to bring...

...(CNS) orchestrates complex processes enabling organisms to control their movements and behavior. These...to study connectomics (how neural circuits are organized and how neurons connect to each other). Combined.... 2. Shepherd, G.M. (2004). In "The synaptic organization of the brain". Oxford University Press, New ...

... that the drug prevents the growth of a living organism. As for multi-drug resistance: definitions vary...vary in the literature, but generally organisms with multi-drug resistance are resistant to three or more...is an intense amount of competition between microorganisms for available resources. This competition drives...

...over-express coding sequences from a given cell type or organism, have been used in a similar manner to Cas9 activators...lines (HEK293T, MCF7, U2-OS, Hela, N2A, 3T3) and organisms (Chavez et al., 2016). To learn more about the...Tuttle M, P R Iyer E, Lin S, Kiani S, Guzman CD, Wiegand DJ, Ter-Ovanesyan D, Braff JL, Davidsohn N, Housden...

...distinct subcellular environments, including the organelles that compose the secretory pathway, endocytic...formation, and proteolytic cleavage. FPs localized to organelles are equally susceptible to these non-native modifications... Lee Snapp. "Fluorescent proteins in cellular organelles: serious pitfalls and some solutions." DNA and...