We narrowed to 610 results for: GAN
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TypeBlog Post...multiplex gene editing in a variety of cells and organisms. However, lengthy genetic payloads and insufficient...processing mechanism, which is conserved in all living organisms and has previously been used for multiplex Cas9...nuclease editing, in virtually any cell type or organisms of interest with only minimal genetic payloads...
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5 Great Apps for Lab Life
TypeBlog Post...me with my research in terms of preparedness, organization, and sharing data. Here are five FREE science...Archives will help both you and your lab stay organized allowing you to upload anything from protocols...came across the Gibco Cell Culture app. Gibco organizes its app into four categories: tools, analytics... -
R Bodies: Membrane-Rupturing Microscopic Tools
TypeBlog Post... can function to break membranes of different organisms. And finally, we demonstrated that R bodies can...speculatively, R bodies might endow divergent organisms with new functions. For inspiration, we can look...Kanabrocki, J. A., Quackenbush, R. L. & Pond, F. R. Organization and expression of genetic determinants for synthesis... -
Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies
TypeBlog Post... work with Drosophila as an experimental model organism. I then described the Gal4/UAS system used by ... survival. For those interested in development, organs of interest, such as the intestines, can be dissected...have!) (4). The power of Drosophila as a model organism is in its amenability to genetic manipulation.... -
Top Requested Lentivirus and AAV of 2016
TypeBlog Post...In July 2016, we launched our Viral Service and began delivering ready-to-use lentivirus and adeno-associated... virus (AAV) to scientists around the world. We began with only a few inventory items offered domestically...PMCID: PMC3785459. 2. Krashes MJ, Koda S, Ye C, Rogan SC, Adams AC, Cusher DS, Maratos-Flier E, Roth BL... -
Communicating Your Science With Help From ComSciCon
TypeBlog Post... at Addgene, ComSciCon, and many more great organizations. At ComSciCon, our primary mission is to strengthen...respective fields. To help attendees launch new organizations that will extend opportunities to practice science... create them. Seek out collaborations with organizations that practice science communication or start... -
Hot Plasmids - May 2022
TypeBlog Post...proteomic mapping in both cell culture and whole organisms (see our 2019 overview). Until recently, the approach...dedicated transgenic line. Using zebrafish as a model organism, the Parton Lab recently created a flexible system...Given that the liver is an immunologically active organ, this advance is likely to afford the ability to... -
A Quick Guide to a Career in Software Product Management
TypeBlog Post...stakeholders, the product managers collate and organize that information and form it into a roadmap--a... features, programs, or tools to build. After organizing the information, a software product manager works...everything running smoothly, you’ll want to be really organized. Often, product managers will have three or more... -
3 Challenges in Plant Synthetic Biology
TypeBlog Post...engineer a specific function in these multicellular organisms. However, the rewards are great if you take the...are needed to increase the body of knowledge. Organizations like Open Plant can then build on this research...plant circadian clock. Petunias have been a model organism for plant research for decades, and the biochemical... -
Plasmids 101: Yeast Vectors
TypeBlog Post...defined as the inability of an organism to synthesize a particular organic compound required for its growth...DNA transformation system.Unlike most other microorganisms, yeast have both a stable haploid and diploid... -
Form Your Own Peer Mentoring Group: A How-To Guide for Scientists
TypeBlog Post...like to start a group mentoring program at your organization, contact me! I can provide a step-by-step, how-to... people from different labs, departments or organizations as it brings a diversity of perspective. Sometimes...transition. Schedule your first meeting and get organized. Discuss your general areas of interest and what... -
dTAG - You're it!
TypeBlog Post...conjugating a CRBN-binding ligand (thalidomide) to an FKBP12F36V binding ligand (ortho-AP1867) via various...molecule is dTAG-13 due its immediate action, potent ligand performance, great depth of degradation, absolute... -
Celebrating 15 Years of Scientific Sharing
TypeBlog Post...celebrate! As we enter this new growth phase of the organization and the expansion of our impact, I’d like to...service and contribution! Leadership On an organizational level, we keep passing our audits with flying...even via Twitter. We love working with local organizations like Just-a-Start, the Northeastern co-op program... -
Addgene: The First Twenty Years
TypeBlog Post...old-fashioned way: through physical outreach, visiting organizations, knocking on doors, and attending conferences...his Blue Flame Award on Bluesky. In 2016, we also began distributing ready-to-use viral vectors from some...across the globe. As the pandemic slowed, Addgene began to think about how we could expand our support for... -
Seven Tips for Using LinkedIn as a Scientist
TypeBlog Post...More…” menu. You can also follow companies and organizations to see their news and job postings. 4) Use ...connections can connect you to people at that organization. This type of connection can help you gather...that is not relevant to business or volunteer organizational work. LinkedIn is not the platform for politics... -
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet
TypeBlog Post...tech transfer team will communicate with your organization’s technology transfer office or other authorized.... rerio (zebrafish), D. melanogaster (fly), C. elegans (nematode), S. cerevisiae (budding yeast), S. pombe.... The name of the Principal Investigator and Organization where your plasmids were first created. From... -
CRISPR in the Clinic
TypeBlog Post...functional correction of the gene in clonally expanded organoids. This work, and many others, pushed the field ... post was originally written in 2014 by Kendall Morgan and updated in 2023 by Susanna Stroik. References...of CFTR by CRISPR/Cas9 in Intestinal Stem Cell Organoids of Cystic Fibrosis Patients." Cell Stem Cell. ... -
Viral Vectors 101: Calcium Sensors
TypeBlog Post...concentrations and transients in living cells and organisms. Unlike chemical indicators, these DNA constructs...constructs can be delivered to intact tissues and organs noninvasively and target a defined population of cells...Genetically encoded fluorescent indicators for organellar calcium imaging. Biophysical journal. 2016 Sep... -
Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells
TypeBlog Post...to deliver a standard AdV vector to the targeted organs or tissues with Cas9 and a gRNA designed to target...67 Ding Q, Strong A, Patel KM, Ng S-L, Gosis BS, Regan SN, Cowan CA, Rader DJ, Musunuru K (2014) Permanent... Kendall Morgan... -
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells
TypeBlog Post...in 720°, i.e. they are on a membrane or in an organelle, then their effective concentration will be much...the membranes into stacked structures including organized smooth endoplasmic reticulum (OSER) whorls. 2)...distorted into dense stacked membranes termed Organized Smooth Endoplasmic Reticulum or OSER if a dimerizing...