...associated genes) system. This popular genome engineering tool offers flexibility, multiplexibility, and... DNA into small fragments approximately 30bp in length and paste them into the CRISPR sequence. Separate...system that scientists have harnessed for genome engineering. The diversity of the CRISPR/Cas systems provides...defense against invading phages. Rising to the challenge - Evolution of anti-CRISPR genes in phages The...who has the strongest will to survive. Genome engineering and future directions CRISPR/Cas inspired genome...Despite the ubiquity of CRISPR/Cas toolkit, genome engineers continue to work diligently and creatively to ...

...impossible. With the genome engineering revolution came epigenome-engineering tools - zinc finger nucleases...Wells D, Ahmed AA, Sauka-Spengler T (2017). Genome and epigenome engineering CRISPR toolkit for probing...the DNA after cytosine removal. Recently, Albert Cheng’s lab developed Casilio-ME, which is based on their...Lysine-specific Demethylase 1 (LSD1) Tatjana Sauka-Spengler's lab has deposited pX330a dCas9-LSD1 for targeted...2017) Targeted DNA methylation in vivo using an engineered dCas9-MQ1 fusion protein. Nature Communications...

... sequence, making it very useful for genetic engineering approaches. Most importantly for this technology...CRISPR/Cas9, TALENs, and ZFNs). However, several challenges still remain, such as making gene edits completely...nuclease technology with the piggyBac™ system, these challenges can be considerably reduced. The piggyBac™ transposon...experience with stem cells to Transposagen's cell engineering team.     Mathew Goodwin is a Technical Sales...Craig, “piggyBac transposase tools for genome engineering.,” Proc. Natl. Acad. Sci. U. S. A., vol. 110,...Sleeping Beauty Transposase Read up on Genome Engineering with FLEx Vectors  Learn how Homology Directed... genome engineering...

...classroom Engineering the Plant Genome Using CRISPR/Cas9 pSiM24: Simplifying Plant Genetic Engineering Resources...Arabidopsis in the classroom has many benefits. Engaging students in active learning through hands-on experiments...system comes with fewer ethical and logistical challenges than does working with animals. Because Arabidopsis...and share resources, we seek out opportunities to engage people in science learning. Whether visiting classrooms...University. In her work, she focuses in public engagement in science and promoting plant science in K-12...

...neuroscience—whatever that entails. Despite being engrossed by the theory and findings of brain science, my... to once again write for fun eventually saw an English minor tacked onto my BSc. With this decision I ...meaningful day job only came into focus when an English tutor noted my eclectic mix of science and arts...so—can prove invaluable in the pursuit of public engagement. Studying science communication is an equally...had to say. Moreover, this subject allowed me to engage in findings relevant to my background (neuroimaging... and the tools to deploy its wisdom remains a challenge. Departments like ours reflect a growing effort...

...100% student engagement. I figured, an engaging card game could help me get the engagement I wanted. I ...Associate Professor at University of Barcelona. The challenge: Making courses on genetic manipulation more hands...could be played when and, depending upon who your challenger was, you might have to drastically alter your...biology, cell biology, microbiology, genetic engineering and strategic knowledge to solve a clinical disease...

...Novel of England by Edward Rutherfurd "A 10,000 year history of southern England. While the length may be...fiction. Two dueling magicians in the 1800s in England who help bring back actual magic (which had been...one. On a purely selfish level, I wanted to be challenged to read some books outside of my comfort zone...life. Though I have an undergraduate degree in English, I had lapsed. Baker’s novel reignited my appreciation...

... of the few members of Congress who holds an engineering degree. It was a great, positive event that I...a PhD Candidate studying Nuclear Science and Engineering at Rensselaer Polytechnic Institute. He also ...groundbreaking research happens, sometimes forgetting how to engage with friends, family, and the public about its ...policy.  He is also chair of the postdoctoral engagement committee and active member of the university's...present at the lab bench," Liu, a UM undergraduate, challenged us. I worried the march would be a simple, unquestioning...scientists must rise up and become more politically engaged. I was proud to be a part of a community that recognizes...face of evidence. Stephanie Hays is moving from engineering synthetic microbial communities at Harvard University...

...expression to a specific neuronal subtype. However, the length of promoter that can be used in an AAV construct... As an alternative, some constructs have been engineered with a double-floxed inverse open-reading frame...transgenic mice Transgenic mouse lines have also been engineered to express various opsin genes [7-9]. The advantage... cell body or an axon terminal also affects the length of time you may need to allow the virus to express...opsin to express and passively diffuse down the length of the fiber before stimulation of the terminals...and must passively diffuse so, depending on the length of your projection, it may take as long as 6-8 ...9):745-52. PMID: 21985008; PMCID: PMC3191888. 9. Zeng H, Madisen L. Mouse transgenic approaches in optogenetics...

...reprogrammed regenerating tissues without using genome engineering. Planarian worms cut into three segments “remember... wild type  (Oveido et al., 2010). One of the challenges of studying bioelectricity is that it can be ...between open and closed states using different wavelength light pulses. Other resources from the bioelectricity...defects, regeneration, medicine, and synthetic bioengineering. References Chernet, Brook T., et al. "Use...growth and form." Annual review of biomedical engineering 19 (2017): 353-387. PubMed PMID: 28633567. Mathews...bioelectricity for regenerative and synthetic bioengineering." Current opinion in biotechnology 52 (2018...

...for many metabolic engineering and synthetic biology goals. In metabolic engineering, the expression of...plasmids in order to study gene function or to engineer cells for specific purposes. Unfortunately, plasmid...interfere with the precision needed in metabolic engineering or other synthetic biology circuits. What is ...Eduardo D. Sontag, and Christopher A. Voigt. "Engineered promoters enable constant gene expression at ...coli using DIAL strains." Journal of biological engineering 5.1 (2011): 10. PubMed PMID: 21787416. PubMed...

...biological systems can hinder our attempts to study and engineer them, but what if we had a simple tool that allowed...phenotype, and quickly profile the resultant hits (Cheng et al., 2014; Wong et al., 2015; Wong et al., 2016...genetic elements including transcription factors (Cheng et al., 2014) and microRNAs (Wong et al., 2015)....can be contacted at [email protected].   References 1. Cheng, Allen A., Huiming Ding, and Timothy K. Lu. "Enhanced...Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex." Nature (2014). PubMed PMID...CRISPR Plasmids Find More Tools for Combinatorial Engineering on Our SynBio Pages ...

... cargo (DNA or RNA) to target cells has been challenging. A variety of vector delivery technologies have...adenovirus for productive infection. AAV vectors are engineered to provide additional safety benefits: they lack...treatment appears to be both safe and effective. Challenges for AAV gene therapy Although AAV-based gene ...promising, scientists are working to overcome key challenges to their efficacy:  Pre-existing immunity: Many...with gene therapy in childhood blindness." New England Journal of Medicine 372.20 (2015): 1920-1926. PubMed...PubMed PMID: 12756417. 5. Chan, Ken Y., et al. "Engineered AAVs for efficient noninvasive gene delivery ...

... while engineering viruses Acr genes can be used as positive selectable markers in engineering viruses... was used to replace genes in the difficult-to-engineer Sulfolobus islandicus rod-shaped virus 2 (Mayo-Muñoz...that have the gene deletion could replicate when challenged with the S. islandicus CRISPR-Cas system. 5. ... proteins are small enough that they could be engineered to “armor” therapeutic phages to protect them...He F, Jørgensen J, Madsen P, Bhoobalan-Chitty Y, Peng X (2018) Anti-CRISPR-Based and CRISPR-Based Genome...

...) signal. While many studies focus on promoter strength as a determinant of gene expression levels, the...multiple adenine (A) nucleotides to the tail of a messenger RNA transcript. The purpose and mechanism of polyadenylation... terminators, such as T7 and rrnB, as well as engineered high-efficiency terminators such as T0. Rho-independent...annotation for 90 bacterial elements. By Ppgardne at English Wikipedia, CC BY-SA 3.0, https://commons.wikimedia.org...initiating the desired cleavage event, although some engineered terminators come close (>95%). For most purposes...detailed analysis of yeast terminators of varying strength. A discussion of the effects of various terminators...

...advs.202202957 Yuan, T., Yan, N., Fei, T., Zheng, J., Meng, J., Li, N., Liu, J., Zhang, H., Xie, L., Ying...researchers in the Keith Joung lab, who were engineering an ABE to reduce its tendency to make off-target...they were using consisted of Cas9 fused to an engineered adenosine deaminase capable of chemically converting...sites. To explore this further, the team started engineering a CBE to be worse at its original job. They ...causing double-strand breaks (DSBs). The Fei-Long Meng Lab did in-depth profiling to compare editing outcomes..., Wang, B., Luo, Y., Cai, Y., Liu, X., Xu, Z., … Meng, F.-L. (2024). C-to-G editing generates double-strand...

...Fujita and Fujii, 2013). They call this method engineered DNA-binding molecule-mediated chromatin immunoprecipitation...immunoprecipitation (enChIP) because any engineered DNA-binding molecule, such as Zinc-finger or TAL proteins...the "in cell" form of this method, in which an engineered DNA-binding molecule such as the CRISPR complex... and identification of associated proteins by engineered DNA-binding molecule-mediated chromatin immunoprecipitation...Identification of telomere-associated molecules by engineered DNA-binding molecule-mediated chromatin immunoprecipitation...genomic regions." Journal of bioscience and bioengineering 108.5 (2009): 446-449. PubMed PMID: 19804873...

...biological engineering. 2010;4(1):1. Pubmed PMID: 20205762. PubMed Central PMCID: PMC2822740. 2. Engler C, Kandzia...Knight TF. Engineering BioBrick vectors from BioBrick parts. Journal of biological engineering. 2008;2:5...neighboring fragments require compatible cohesive ends Engler et al., 2008 Gibson Cloning Gibson assembly method...MXS-chaining method have used the technique to engineer various constructs with applications in flow cytometry...

...to the challenge. Find the PHP Plasmids Here! Their Solution: Use the CREATE method to engineer two new...3) they have limited toxicity. However, a key challenge of using AAVs for neuroscience research is the...AAV.PHP.S targets the PNS since it was actually engineered to target the CNS. How well do these AAVs transduce...takeaways: Two new AAV capsids (figure 1) were engineered with the CREATE method (figure 2) to allow for...References 1. Chan, KY, Jang MJ, Yoo BB, et al. Engineered AAVs for efficient noninvasive gene delivery ...

...Aequorea victoria. It has a fluorescent emission wavelength in the green portion of the visible spectrum ...study of dynamic, physiological processes. Re-engineering GFP to increase its range of color and application...its sequence was elucidated, scientists began engineering new versions of GFP through mutagenesis in order...iterations of fluorophores are constantly being engineered. Table 1 lists a few common fluorescent proteins...component (cyan derivatives) Y66H Blue-shifts the wavelength (blue derivatives) Y145F Increases quantum ...at high concentrations T203Y Red-shifts the wavelength (yellow derivatives) A206K Interferes with ...