Addgene

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Showing: 201 - 220 of 259 results

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Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

Type

Blog Post

...experiment. Certain scientific communities have a little more leeway in what they can name a gene and many...
Designing Your Chalk Talk for the Academic Job Interview

Type

Blog Post

...eating one before your chalk talk. Bring your own bottle of water. If possible, write down your short aims...
The PAM Requirement and Expanding CRISPR Beyond SpCas9

Type

Blog Post

...Ibraheim R, Gupta A, Sasaki KE, Wu T, Donohoue PD, Settle AH, Lied AM, McGovern K, Fuller CK, Cameron P, ...
Important Considerations in Optogenetics Behavioral Experiments

Type

Blog Post

...as well. Inhibition of neuronal activation is a little more complicated since basal neuronal activity ...
Data Freedom: The Expansion of Data Sharing in Research Publications

Type

Blog Post

...searching and extraction of information. Looking a little further ahead, journals may move towards encouraging...
Plasmids 101: The Promoter Region – Let's Go!

Type

Blog Post

...etc) require unique promoters and there is very little crossover. Generally speaking, promoters in bacteria...
Antibodies 101: Introduction to Gating in Flow Cytometry

Type

Blog Post

...principles of gating, you’ll first need to learn a little bit of theoretical background on the different ...
CRISPR 101: Homology Directed Repair

Type

Blog Post

...modifications (~1-50 base pairs). Small edits require as little as 30-50 bases for each homology arm, but keep ...
CRISPR 101: Validating Your Genome Edit

Type

Blog Post

...(the 3rd sequencing trace). While it requires a little extra wet lab work, this is still a great option...
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

Type

Blog Post

... ML, Agbandje-McKenna M, Jude Samulski R (2010) Little Vector, Big Gene Transduction: Fragmented Genome...
Multiple Plasmids at a Low Price: Kits from Addgene

Type

Blog Post

...you can create custom RNA binding proteins in as little as 3 days. Abil et al., 2014 show that you can ...
10 Steps to a Perfect Science Talk

Type

Blog Post

...learn new things, so rehash common knowledge as little as possible. Don’t use a lot of jargon in your ...
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

Type

Blog Post

...evade immune detection because they produce very little or no viral protein. To solve this problem, two...
Treating Muscular Dystrophy with CRISPR Gene Editing

Type

Blog Post

...joining (NHEJ) pathway. It’s estimated that very little dystrophin correction (about 4%) is needed to see...
Plasmids 101: Common Lab E. coli Strains

Type

Blog Post

...work! But let’s take a moment to recognize your little prokaryotic minions that carried out the labor-...
Viral Vectors 101: Optogenetic Tools

Type

Blog Post

...range of wavelengths. While this may give you a little flexibility when activating the optogenetic tool...
How to Design Your gRNA for CRISPR Genome Editing

Type

Blog Post

...importance in design – an optimized sequence will do little if it is in the wrong place, but because the target...
Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

Type

Blog Post

...We all know that in the lab there are often little tricks that are essential for experiments but that...
Is this the right place for me? 8 tactics for choosing a lab

Type

Blog Post

...? Threats to professional status – including belittling opinions, public professional humiliation, accusations...
Recombinase-based State Machines Enable Order-dependent Logic in vivo

Type

Blog Post

...conditions drive certain cellular behaviors, but little is known about the order or timing of these factors...

Showing: 201 - 220 of 259 results

Results per page:

Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

Designing Your Chalk Talk for the Academic Job Interview

The PAM Requirement and Expanding CRISPR Beyond SpCas9

Important Considerations in Optogenetics Behavioral Experiments

Data Freedom: The Expansion of Data Sharing in Research Publications

Plasmids 101: The Promoter Region – Let's Go!

Antibodies 101: Introduction to Gating in Flow Cytometry

CRISPR 101: Homology Directed Repair

CRISPR 101: Validating Your Genome Edit

Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

Multiple Plasmids at a Low Price: Kits from Addgene

10 Steps to a Perfect Science Talk

Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

Treating Muscular Dystrophy with CRISPR Gene Editing

Plasmids 101: Common Lab E. coli Strains

Viral Vectors 101: Optogenetic Tools

How to Design Your gRNA for CRISPR Genome Editing

Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

Is this the right place for me? 8 tactics for choosing a lab

Recombinase-based State Machines Enable Order-dependent Logic in vivo

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