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We narrowed to 755 results for: rest

Showing: 201 - 220 of 755 results

  1. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...in nearly any neuroscience project or topic of interest. Despite its power and versatility, the technique...experiment to test for sufficiency of your circuit of interest in promoting reward is a self-stimulation experiment...implantation of the optical fiber into the region of interest 3) Behavioral experimentation I’ll discuss some...construct in the brain region and cell type of interest is obviously of critical importance to any optogenetic...promoters and other genetic manipulations to help restrict expression to a specific neuronal subtype. However...constructs are injected directly into the region of interest using stereotaxic surgery (stereotaxic surgery...the proper coordinates of your brain region of interest (as shown in the figure below). Since most of ...

  2. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...technologies and spaces where interested parties can do research. This interest manifested itself in the creation...Over the decades science has become increasingly restricted to academic and industrial labs, but recently...for our computer scientists, many of whom are interested in biology but don’t really know how to start...pipeline. It’s possible that we’ll find something interesting and contribute it back to the kittybiome project...website. Markerspaces differ depending on the interests of their community, and biology is not yet common...recognition, he returned to school to merge his interests in biology and computing. In 2009, he founded ...

  3. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type
    Blog Post
    ...hierarchical approaches using different Type IIS restriction enzyme specificities at each step. Factors such...limitations through the use of engineered Type IIS restriction enzymes and careful choice of junction sequences...involves the simultaneous activities of a Type IIS restriction enzyme such as BsaI, and a DNA ligase such as...receives the assembly after similar Type IIS restriction enzyme cutting results in 5’ four base overhangs... provide optimal reaction temps for both the restriction enzyme and ligase, thereby enhancing the ordered...destination construct and functioning Type IIS restriction enzyme and DNA ligase, any single omission should...re-engineering of the original BsaI-HF® Type IIS restriction enzyme and the completion of T4 DNA Ligase fidelity...

  4. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ... Perhaps interest has simply waned. When attendance slumps below a certain level or interest wanes off...things to think about. Choosing a topic and gauging interest The first thing to do if you’ve thought about ...club is to find a topic and seeing if others are interested. You don’t want to pick a topic and have no one...directly involved in the project.  Once we gauged interest from relevant parties, and the company at large... stay caught up and to allow those who may be interested in attending to see what they are missing!  So...institutional inertia drives the meeting rather than interest. These are the journal clubs where participants...

  5. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...presence and quantity of DNA, RNA or a mutation of interest. Together SHERLOCK and DETECTR demonstrate the...be programmed with crRNA to target an ssRNA of interest, for example, a sequence specific to a virus or...Csm6 can cleave ssRNA complementary to a crRNA of interest (Niewoehner and Jinek, 2016) and is conveniently..., binding of Cas13 to the programmed region of interest would lead to both the cleavage of a Cas13 specific...flow strips, the presence of your DNA or RNA of interest within a given sample is simply determined by ... When Cas12a-cRNA base pairs with the dsDNA of interest, the DNase activity of Cas12a is initiated. Surrounding... signal designates the presence of your DNA of interest, in this case HPV. As proof of concept, the lab...

  6. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    ...you do will be determined by the topic you’re interested in studying, but, in today’s post, we’ll discuss...short blog post, but some examples I’m personally interested in include: intracranial self-stimulation (ICSS...neurobiology of addictive diseases, which means we are interested in circuits that mediate drug taking behavior...basal neuronal activity in your brain region of interest could be low or highly variable without the behavioral...around a behavioral test in which your circuit of interest is putatively involved in regulating the behavioral...behavioral output. For example, if you are interested in studying a brain region that may be important in...Jeanne Kreek at the Rockefeller University.  His interests include the role of learning and memory in opioid...

  7. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

    Type
    Blog Post
    ...is often our first clue about where the gene of interest might be located. By easily using BLAST to check...additional verification experiments: Primer and restriction digest design Some regions of plasmids are difficult...present in the plasmid, we might need to perform a restriction digest to confirm the size of the plasmid and...including our most popular primers and certain restriction enzymes, but you will also be able to create ...annotations and design additional experiments like restriction digests. We’ll have a tutorial showing all of...Basic Molecular Biology Protocols Diagnostic Restriction Digest Protocol ...

  8. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...different plasmids containing the same protein of interest tagged with different fusion proteins for different...dye or other functional ligand, your protein of interest will end up linked to whatever molecule you choose...useful ligand, gray structure is the protein of interest, and black is the fusion tag or labeling system...attach a ligand to a peptide tag on your protein of interest or using non-natural amino acids to directly incorporate...minimally-disruptive genetic tag on the protein of interest. Table 1: Summary of popular self-labeling tag...orthogonal self-labeling tags on different proteins of interest (or one self-labeling tag and a fluorescent protein...sample to label sub-populations of your protein of interest. For example, the Cohen lab used HaloTag for their...

  9. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

    Type
    Blog Post
    ...I would be interested in joining the audit committee about five years ago. I was interested in what she...there will always be an interest in sharing, there will always be an interest in protecting intellectual...the open science movement is that it provides interesting and unique challenges for intellectual property...that balance is, and will continue to be, an interesting legal and professional challenge.Paratore: The...

  10. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    ...and see one of two results for your antibody of interest: two clear populations or a huge smear across ...on the smeary dot plot shows the population of interest. Created with biorender.com.   In such cases... all cells that are positive for the marker of interest. MFI would be higher for a signal peak that shifts..., you can still continue gating populations of interest with normal gates, just based on a histogram plot...peis the baseline expression of the protein of interest. Created with biorender.com. The plot above...positive and negative populations for your marker of interest, a dot plot would serve you well. Percentage of...

  11. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    ...using in the experiment except for one marker of interest. This allows you to more easily and accurately...schematic to easily determine where your cells of interest are (B).   Using FMO controls In an experiment...virtually identical to the antibody for your marker of interest. It will be from the same host species, have the...isotype antibody will not target your marker of interest. Using isotype controls An isotype sample is prepared...using in the experiment except for your marker of interest, and then add in your isotype control for that...positive cells are truly positive for your marker of interest (Fig. 3).   Figure 3: Isotype flow plot...

  12. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type
    Blog Post
    ...depending on your interests. You can look at something as basic as survival. For those interested in development...straightforward. Basically, you place your gene of interest in a plasmid and get the vector incorporated into...transgene. One such marker is the white gene, which restores eye color to fly lines where the endogenous white...fly and downstream experiments Once your gene of interest is cloned into a plasmid, you can send your plasmid...development, organs of interest, such as the intestines, can be dissected at various times throughout the lifespan...

  13. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    ...identify. Improved Restriction Enzyme Handling - SnapGene’s software positions restriction enzyme annotations...paste functionality. Restriction Enzymes Here you'll find a table of restriction enzymes that cut the...previous mapping software and now includes Type IIS restriction enzymes that are commonly used for Golden Gate...clicking on column heads. Clicking on a particular restriction enzyme here also highlights it in the “Map” and...

  14. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    ...willing to step up and be matched with the many interested mentees. So I experimented with a group mentoring...available to go around. My advice has been to find 5 interested peers and form your own peer mentoring circle... Find 5-8 colleagues that you think might be interested and willing to commit to the group. The group...problem. For example, a group of people who are all interested in making a transition. Schedule your first meeting...and get organized. Discuss your general areas of interest and what you want to cover during the year. Suggest...Invite guests from a lab, career or industry of interest – learn about their career paths and get their...

  15. dTAG - You're it!

    Type
    Blog Post
    ...compounds that enable selective removal of proteins-of-interest from the proteome. Rather than inhibiting protein...inhibitors including rapidly depleting a protein-of-interest, increasing target selectivity, overcoming resistance...functionality of your fusion chimeras For each protein-of-interest, it is important to determine which terminus can...C-terminal fusions and degradation in a cell line of interest prior to starting knock-in experiments. Test dTAG...degradation of the FKBP12F36V-fusion chimera of interest. In the cell lines we have evaluated to date, ... Cancer Institute. He is a chemical biologist interested in targeted protein degradation, and lead author...

  16. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    ...primers to detect your targeting vector or gene of interest. 2. Breed your parental mice to generate F1 Once...parental mice were all homozygous for your genes of interest, your F1 mice will be carriers at those alleles...harbor homozygous alleles at both of your genes of interest. About 1 in 4 mice will be homozygous for the ...The shorter the distance between the two loci of interest on the chromosome, the less likely crossing over... with a strain that expresses a floxed gene of interest. Cre may be expressed globally (in every cell ... for expression of Cre, loxP, and your gene of interest. To ensure that the effects you see in your experiment...

  17. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    ... your gene of interest pLenti-puro (Plasmid #39481) You can clone your gene of interest into this tetracycline...vectors that are used for gene regulation, gene of interest expression, knockout, and screening. There are...gamma-Retroviral Transfer Vectors   Expressing your gene of interest pMKO.1 GFP (Plasmid #10676) Retroviral vector ...vector for cloning and expressing your gene of interest with GFP expression.  Cloning and expressing RNAi pMKO...analysis of cells pre-treated with a compound of interest. Lentiviral vectors Popular Lentiviral Transfer...

  18. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...Deliver CRISPR library to cells of interest. Select for cells of interest (positive or negative selection...Next-generation sequencing (NGS) to identify genes of interest. Figure 1: Overview of in vitro AAV CRISPR...serotypes that have a known tropism for your cells of interest — for example, AAV8 for kidney cells — can help...solutions have been found to get around this restriction. The most obvious workaround is to use a multi-plasmid...deliver the Cas protein altogether. Platforming AAV Interest in interrogating and mapping the genetic landscape...

  19. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ... to meet people with similar interests! Especially as someone interested in doing science outreach, not...professional development opportunities for researchers interested in connecting with the public. My experiences...amazingly supportive. I talked to them about my interests and my ideas all the time. Once, I had this crazy...The article submitted was hilarious, full of interesting information, and received a lot of attention ...University School of Medicine. She is particularly interested in science communication and fundamental molecular...

  20. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...Rather than try to directly detect your gene of interest, scientists have instead developed novel methods...select for cells that are expressing your gene of interest at high levels. Unlike vectors that express screenable... If you are looking to co-express your gene of interest along with a fluorescent protein or selectable...these plasmids you would simply clone your gene of interest into the multiple cloning site up or down stream...reporter genes above with your gene or genes of interest. Some of the plasmids listed in the table above...peptides could be PCR-cloned between your genes of interest and you can then insert the whole multicistronic...
Showing: 201 - 220 of 755 results