....1111704108. Epub 2011 Aug 9. PubMed. Find the Plasmids @Addgene: Plasmids for KD, R, B2, and B3 recombinases from...frequency of 1.2 per megabase in mammals), leading to DNA damage and developmental aberrations. In multiple...10.1038/ni0707-665. PubMed. Growth inhibition and DNA damage induced by Cre recombinase in mammalian cells...

...ddPCR) Historically, a quantitative DNA hybridization method (DNA dot blotting) had been used to titrate... as opposed to purified DNA. This may be mitigated by using purified viral DNA as the template instead...the category: ELISA...qPCR...Digital droplet PCR...DNA dot blot...Transduction assay...SDS-PAGE...Electron...concentration of viral genomes (by qPCR or other DNA quantification methods - see below), since each viral...concentration. We regularly make and validate fresh plasmid standards. We validate our absolute quantification... digital PCR system for absolute quantitation of DNA copy number." Analytical chemistry 83.22 (2011): ...

...skin, blood and semen samples. Ma et al. designed plasmids containing Cas9 and two different gRNAs, and electroporated...oocytes, thus allowing repair to take place before DNA replication. Indeed, they observed that 41/42 embryos...editing.’ While repair using endogenous chromosomal DNA is appealing for heterozygous mutations, it will ... homozygous mutations. Moreover, introducing new DNA seems impossible with current tools, thus quieting...CRISPR Resources on Addgene.org Browse All CRISPR Plasmids Check Out Our CRISPR Guide Pages ...

... its reported cleavage site on the complementary DNA strand. And this is why we love working with researchers... have in order to scan along the double-stranded DNA, recognize a PAM sequence, allow the complementary...base pairing between the guide RNA and the target DNA strand, and then activate two different endonuclease...This structure captures the displaced non-target DNA strand and shows the HNH nuclease domain swung around...juxtaposition to its cleavage site on the target DNA strand. In addition, this model also displays the...Guide Page CRISPR/Cas9 History Browse All CRISPR Plasmids and Resources ... technology to 4th graders, check out Jennifer Doudna’s new book A Crack in Creation. Making models more...

... Addgene/Seeding Labs plasmid grant and plans to use the grant to order plasmids she can use as positive...similar grants back in June, Gladys will be using plasmids in creative ways that we wouldn’t necessarily ...commonly used in Ghana, there is little screening for DNA markers like the presence of high risk (HR) Human...HR HPV types, predominantly HPV 16 and 18.” HPV plasmids as positive controls To start her work, Gladys...cytological tests currently in use. Gladys will use plasmids containing a variety of HPV genes as positive ...incredibly happy to hear that Gladys will be using plasmids in a way that can potentially have a direct impact...awardees We will be opening the Addgene/Seeding Labs Plasmid Grant to a third round of applicants at the start...

...the bay area to create a plasmid rap video for us (If you’re new to plasmids, we highly recommend checking...about colony collapse disorder.     5. Plasmid Rap Plasmid rap video made with support from Addgene....incredibly important role in solving the structure of DNA. 2. The Immortal Rap of Henrietta Lacks Students...Podcasts Find Great Educational Content in our Plasmids 101 Series Additional Resources on Addgene.org...

...Read our plasmids 101 blog series Resources on Addgene.org Browse our zebrafish CRISPR plasmid collection...Creating transgenic organisms by introducing foreign DNA into the genome is useful for a variety of applications...transposon called Tol2 to randomly integrate foreign DNA into the genome. Non-random integration into specific...facile CRISPR/Cas9-mediated insertion of foreign DNA. Despite these caveats, several features of zebrafish—including...collection Read our molecular biology reference Find plasmids for your research ...

...Feng Zhang’s lab at MIT, the use of customizable DNA-binding domains based on transcription-activator-... (blue) light switch. Zhang’s team devised the plasmids now available in Addgene’s repository for use ...biological processes and disease states. While LITE plasmids have clear application to anyone with an interest...

...Isotype switching is caused by a specialized type of DNA recombination. Heavy chain constant genes are clustered... expressing a different isotype, the intervening DNA sequence is deleted, which places the variable region...    Figure 2: Isotype switching requires DNA recombination of the antibody gene locus. B cells...in the heavy-chain constant domain gene cluster. DNA recombination can delete intervening heavy-chain ...Resources on Addgene.org Browse Addgene’s Antibodies plasmid collection ...

...sexual reproductive life cycles. One can inject plasmid DNA in the eggs to get transgenic animals. They might...of molecular biology, many scientists do a lot of DNA work but never get to actually manipulate the organism...plans and in particular, that they all share the plasmids created under this programs via Addgene. New organisms...such that the two plants share RNA, proteins, and DNA. Euprymna scolopes (Joshua Rosenthal) The Hawaiian...efficiently transform these cells which don’t like foreign DNA at all (maybe because they have only 1,700 genes ...

...genome is lethal. If this DSB occurs on a plasmid, the plasmid will be eliminated from the bacterium, which...sequence-specific, antimicrobial, plasmid-based, CRISPR systems (Find the Citorik Plasmids here). In both cases, ...genes in E. coli using plasmid and phagemid delivery systems. Phagemids are plasmids containing a phage origin...sequence results in cell death. Plasmid targeting may result in plasmid loss and antibiotic resensitization... three essential processes: cell wall synthesis, DNA replication, or protein synthesis. Because they target...this resistance gene being encoded by a high-copy plasmid. In the case of the genomically encoded quinolone...resistance, mediated by a single base pair change in DNA gyrase, CRISPR phagemids were cytotoxic only for ...

...the chimera are encoded by DNA. Since researchers can generate almost any DNA sequence in the way that ...instance, Lifeact-mTurquoise2 (Plasmid #36201) or mScarlet-tubulin (Plasmid #85045). We often use restriction...protein over-expression artifacts The introduction of DNA that encodes a fusion protein adds protein to the...advisable to first express the fusion protein from a plasmid-based system to assess whether the chimera is still...Download the fluorescent proteins 101 eBook Read our plasmids 101 blog series Resources on Addgene.org Browse...protein fusions Browse the fluorescent proteins plasmid kits ...measures homodimerization of FPs targeted to the endoplasmid reticulum based on the appearance of OSER whorls...

... awarded plasmids from Addgene as a successful applicant to the Addgene, Seeding Labs Plasmid Grant. For...Learn about the Addgene, Seeding Labs Plasmid Grant Read about Plasmid Grant Recipient, Gladys Kaba Resources... fellow nonprofit, Seeding Labs, to distribute plasmids to researchers in developing regions and thereby...accelerate their research. Kwabena came across the plasmid grant in Seeding Lab’s Newsletter and recently ...plans to use his research experiences as well as plasmids from Addgene to bring new opportunities to Ghanaian... Kwabena applied for the Addgene, Seeding Labs plasmid grant. 7:12 - 7:11 : Kwabena's work in Christopher...the University of Edinburgh - synthetic biology, DNA assembly methods, and engineering cellulose degradation...

...exact Cas9 plasmid used by Jinek et al. 2012, or browsing for the latest AsCas12a plasmids, Addgene and... hand, generates staggered DNA nicks at its target in cis and cleaves ssDNA in trans. In sum, CasPEDIA...CasPEDIA documents the target substrate (DNA/RNA and strandedness) along with its action on the substrate (nick...find a link to search Addgene's collection for plasmids containing that enzyme, and embedded in the References...lists on every page you can find direct links to plasmids from the article if they're available through ...problem: CasPEDIA, a new resource from Jennifer Doudna’s lab at the Innovative Genomics Institute. What...frequently used Cas enzymes) cleaves both strands of its dsDNA target in cis and has no trans activity. AsCas12a...

...working with the Rare Genomics Institute to provide plasmid grants to researchers working on rare diseases ...Richard Gronostajski who each are being awarded free plasmids form Addgene to accelerate their research. Read... on to learn how these researchers plan to use plasmids from Addgene to advance their research on diseases...Prof. Videira and collaborators will use Addgene plasmids to immortalize CDG patient-derived immune cell...understanding the structural determinants of protein-DNA interactions and how these interactions regulation...Nfix-negative neural stem cells. The lab will use plasmids from Addgene to construct NFIX expression vectors...

...CRISPR/Cas9 ribonucleoprotein complex with linear DNA as repair templates which reduces time to generate...repair templates with homology arms of 35-80 bp, and DNA double-strand breaks up to 54 bp from the desired...in knock-in efficiency, presumably by channeling DNA breaks into the homologous recombination repair pathway.... elegans. These modular toolkits build single plasmid targeting vectors that encode the gRNA transcript... C. elegans, visit Addgene’s C. elegans CRISPR plasmids page. Future perspectives It is interesting to...Single-Stranded and Long, Partially Single-Stranded DNA Donors in Caenorhabditis elegans. Genetics 210:781...5′ Modifications Improve Potency and Efficacy of DNA Donors for Precision Genome Editing. BioRxiv. https...

...biotinylating proteins with BirA. You can find plasmids that contain both AviTag and BirA at Addgene! ...labeling Biotin can be added to either terminus of a DNA sequence during a Polymerase Chain Reaction (PCR)...Photobiotin can be used to non-selectively label DNA and RNA. Photobiotin acetate consists of a biotin...electrophoretic mobility shift assay to examine specific dsDNA, ssDNA or RNA–protein interactions.” Nucleic Acids ...

...Addgene.org Browse our CRISPR plasmids for visualization Find plasmids for your research Read our CRISPR...protein (dCas9) is well known for its ability to bind DNA targets without changing them. Thus, it has been ...cells. Based upon this, they chose an intergenic DNA region (IDR3) as a reference loci to measure inter-locus..., give CRISPR-Sirius a try. Find CRISPR-Sirius plasmids at Addgene. References Ma, Hanhui et al. “Multicolor...

... target plasmid and pX330-sgRNA plasmid (Mashiko et al. 2013). The pCAG-EGxxFP target plasmid contains...pCAG-EGXXFP plasmid along with the individual gRNAs in their PX330 plasmids (or similar plasmids for gRNA... (c) The pCAG-EGxxFP target plasmid was co-transfected with pX330 plasmids containing sgRNA sequences ...degradation that can set you back days. Working with plasmid DNA is much easier and doesn’t require the extra ... system floating around, and many pre-designed plasmids containing these variants ready for purchase. ... or you can order them yourself through whatever DNA supply company you currently use. You can also find...multi-cloning site (MCS) between EGFP fragments. The pX330 plasmid contains humanized Cas9 expression cassette and...

...of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles.  ...indels after DNA repair. B) M. abscessus is first transformed with Cas9 on an integrative plasmid containing... or want to swap out the constant domains, the plasmids for the heavy chain and light chain are also available...ubiquitination and degradation.   Find the CRBNmidi plasmid here!   Kroupova, A. et al. (2024). Design of ...minimal background.   Find these C. elegans mScarlet plasmids here!   Cao W.X., Merritt D.M., Pe K., Cesar ...disruption in Mycobacterium abscessus using a dual-plasmid CRISPR/Cas9 system By Aryana Khosravani Deborah...Deborah Hung’s lab has developed a novel dual-plasmid CRISPR/Cas9 system for gene disruption in Mycobacterium...