AAV Titers: Where Do They Come From And What Do They Mean?
Type
Blog Post
...point is there are many ways to quantify the composition of a viral vector solution, and generally these...only 20% of recombinant AAV2 particles were full, compared to 50% of wild-type AAV2 stocks (Grimm et al.,...understand what these values mean and how they can be compared.
Figure 2: Label that comes on Addgene...affected by many experimental factors. To list some complicating factors for quantifying AAV:
qPCR efficiency...the AAV genome, caused by repeats and self-complementarity of the AAV genome and the ITRs.
qPCR efficiency...individual sample, this will reduce convenience and comparability because each sample with be quantified with ...linear region of the assay. This may be due to competition for binding sites between the primers and the...