...are addressed with ddPCR technology (Hindson et al., 2011, Hindson et al., 2013), which has been shown ...2011): 8604-8610.4. Hindson CM, Chevillet JR, Briggs HA, Gallichotte EN, Ruf IK, Hindson BJ, Vessella RL,... therapy 6.7 (1999). PubMed PMID: 10455443. 3. Hindson, Benjamin J., et al. "High-throughput droplet digital...

...Pinnelli M, Ansari R, Harper S, Jackson DA, McRae R, Pooley R, Wilkinson P, van der Meer D, Dow D, Buser-Doepner...of TSS for best results but up to 400 bp works reasonably well.  Best of luck on your experiments!  Many...Housden BE, Perrimon N, Collins JJ, Church G (2016) Comparison of Cas9 activators in multiple species. Nat Methods...doi.org/10.1038/nmeth.3871 Gilbert LA, Horlbeck MA, Adamson B, Villalta JE, Chen Y, Whitehead EH, Guimaraes...

...favorably to other methods. In a head-to-head comparison with Cas9, Cas9 modified 9/12 known off-targets... C., Rees, H. A., Packer, M. S., Badran, A. H., Bryson, D. I., & Liu, D. R. (2017). Programmable base ...doi.org/10.1038/nbt.3803 Koblan, L. W., Doman, J. L., Wilson, C., Levy, J. M., Tay, T., Newby, G. A., Maianti... Bohnuud, T., Fernandez, T. P., Gehrke, J. M., Grayson, P., Lee, K. D., Ortega, M. A., Sawyer, C., Schwaegerle..., Lapinaite, A., Newby, G. A., Thuronyi, B. W., Wilson, C., Koblan, L. W., Zeng, J., Bauer, D. E., Doudna...Levy, J. M., Yeh, W.-H., Zheng, C., Newby, G. A., Wilson, C., Bhaumik, M., Shubina-Oleinik, O., Holt, J....

...cells are great model organism not only for the reasons above but because they actually share many biological... the study of neurological diseases, such as Parkinson’s disease (PD). PD is primarily characterized by...pancreas and produce different levels of insulin (Hesselson et al., 2009). The worm (Caenorhabditis elegans...nature391.6669 (1998): 806. PubMed PMID: 9486653. Hesselson, Daniel, et al. "Distinct populations of quiescent...From the baker to the bedside: yeast models of Parkinson's disease." Microbial cell 2.8 (2015): 262. PubMed...

...10.3390/antib8020028  Bird R, Hardman K, Jacobson J, Johnson S, Kaufman B, Lee S, Lee T, Pope S, Riordan... (Sandomenico et al., 2020). Figure 1: Comparison between the IgG antibody and scFv.   What...doi.org/10.1371/journal.pone.0189964  Huston JS, Levinson D, Mudgett-Hunter M, Tai MS, Novotny J, Margolies... made by molecular evolution 1 1Edited by I. A. Wilson. Journal of Molecular Biology 275:245–253 . https...

...designing and testing your AAV.  Figure 2: Comparison of AAV serotypes 1, 2, 5, 6, 8, and 9 in two ...Taschenberger et al., 2017).     Figure 3: Comparison of two astrocyte selective promoters demonstrating...Sortwell, C. E., Hauswirth, W. W., Boye, S. E., & Manfredsson, F. P. (2017). Rationally Engineered AAV Capsids...vector-mediated transduction of the corticospinal tract: Comparison of four promoters. Gene Therapy, 28(1–2), 56–... Jason Nasse...

...PMC2693335. Williams JA, Luke J, Langtry S, Anderson S, Hodgson CP, Carnes AE. Generic plasmid DNA production...and at high copy numbers (Williams, Carnes, and Hodgson, 2009). More research is ongoing to determine plasmid...90057-7. PMID: 2544913. Cook KS, Luo J, Guttman A, Thompson L. Vaccine Plasmid Topology Monitoring by Capillary...90060-6. PMID: 6323019. Williams JA, Carnes AE, Hodgson CP. Plasmid DNA vaccine vector design: impact on...

...2013), or rescue of mutant phenotypes (unc-119) (Dickinson et al., 2013). These approaches allowed effective...the development of the self-excising cassette (Dickinson et al., 2015) made this step as simple as heat-shocking...e193–e193 . https://doi.org/10.1093/nar/gkt805 Dickinson DJ, Pani AM, Heppert JK, Higgins CD, Goldstein...sequences https://doi.org/10.17912/W2237D Farboud B, Severson AF, Meyer BJ (2018) Strategies for Efficient Genome...Krishnamurthy PM, Gneid H, Edraki A, Watts JK, Sontheimer EJ, Mello CC (2018) 5′ Modifications Improve ...

...many stages of synthesis and degradation. For this reason it is virtually impossible to determine specific...parameter to consider is the contrast ratio- a comparison of PA-FP brightness before and after photoactivation...extraordinary molecular detail.   References 1.Patterson, George H., and Jennifer Lippincott-Schwartz. ..., Samuel T., Thanu PK Girirajan, and Michael D. Mason. "Ultra-high resolution imaging by fluorescence ...Photoactivatable FPs in Empty Backbones in the Michael Davidson Collection Find Photoactivatable FPs for Single...

...contributed by Kurt Swanson, husband of Addgene Director of Biology, Lianna Swanson, and researcher at ...in food products). I picked this post for a few reasons: 1) I think phage are super cool and have considered...proteins that could be useful for a wide variety of reasons. 3) I would love to see more posts like this on...

... spend a few days editing and later looking for songs and sounds to synchronize with the images thereby...the hardest part of the process was to embed personality in pieces of plastic, but it’s gotten easier ...than a piece of plastic for us now). With the personality in place, everything now is about the plot. This...How You Can Use Video to Share Your Research 5 Reasons to Use Reddit for Science Communication 10 Ways...

...to improve, and they’re also clear about how the person is doing overall. Employees hate to wonder what...employee. Make sure to focus on the action, not the person - intent is usually good. Don’t pretend something...standard format not only enables you to discuss a person's performance or what has been learned, it helps... effective over time if you take it seriously.  Person who will receive feedback does a self-assessment...

... ask a lot of questions. Personality and style conflicts are a common reason for scientists to change ...budgeting, equipment acquisition, public speaking and personnel management?  If you are thinking about a non-academic... you can help it. Observe as much as you can in person. Rotate in the lab, if possible. Attend a lab meeting...

...use Bioluminescence Resonance Energy Transfer (BRET) were developed by the Johnson Lab . Characteristics...CRISPR labeling chromosomal loci in genome Thoru Pederson’s lab at the University of Massachusetts Medical...lab further modified sdChr- a blue-shifted channelrhodpson from freshwater green alga identified by Ed...Nano-lanterns rely on the principle of bioluminescence resonance energy transfer (BRET), whereby photons emitted...-tagged human ORF clones to allow testing and comparison of different cell-free expression systems; the...understanding complex gene regulation (Bert et al 2000, Johnson et al 2004). So if you are looking for a luciferase... et al. Plasmid. 2000. PubMed PMID: 10964627 Johnson, et al. Mol Cell Biol. 2004. PubMed PMID: 15340054...

...end; cause, effect; lesson, learning. Storytelling is a crucial part of interpersonal communication, which... it came time to add author details a different person in the lab wanted to be the first author. When ...protagonist will be you. Make sure to highlight what lessons you’ve learned and what knowledge you’ve gained...

...   In fact, there are a lot of good reasons to stay and good reasons to go.  Here are some questions and...especially race and gender) Interpersonal issues can create interpersonal issues (one bad apple can change...

...process samples at the picnic table before dark, a bison wandered into our campsite. We had to abandon our...our samples and move farther away until the bison meandered off. In the end we were able to safely get...get back to our lab work. Figure 3: When a bison wanders into the campsite, leave your samples behind...ThermoCas9 from the van der Oost lab. Phylogenetic comparison indicates that like GeoCas9 and ThermoCas9, IgnaviCas9...

...Although CRISPR/Cas9 is all the rage, they had another reason to test this system. It’s difficult to determine...require multiple transduction events. For these reasons, iTOP is best suited to binary systems in which... size associated with viral vectors. For these reasons, iTOP, especially in tandem with genome editing... VP22 nuclear homing an artifact? Lundberg M, Johansson M. Nat Biotechnol. 2001 Aug;19(8):713-4. PubMed...

... T. M., Edwards, A. M., Healy, L. M., Robertson, J., & McPherson, P. S. (2019). Implementation of an antibody...perform as well in other conditions. For these reasons, it is essential to test the selectivity of your...cannot prove antibody selectivity, and for that reason, it is best practice to combine correlation with...C., Bandrowski, A., Virk, H., Edwards, A. M., McPherson, P., & Laflamme, C. (2023). Scaling of an antibody...

...Thanks to our guest blogger Cameron MacPherson! Cameron R. MacPherson is Lead Data Scientist for the Milieu...post was contributed by guest blogger Cameron MacPherson at the Institut Pasteur CRISPR software and the... by their focus on specific organisms. For this reason I generally recommend that laboratories that have...CRISPR technology to a new organism: For obvious reasons you can rule out any tool that limits you to a ...