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We narrowed to 353 results for: SAM activators

Showing: 201 - 220 of 353 results

  1. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type
    Blog Post
    ...proteins in tissues, cells, and biofluids. Recently, Sam Myers, a Scientist in the group, developed a CRISPR-based...and RNA sequencing of complex heterogeneous RNA samples. Furthermore, such high processivity enzymes and...specific genomic loci. He made use of catalytically inactive dCas9 by fusing it to an engineered peroxidase...Using these lentiviral plasmids, catalytically inactive Cas9 (dSpyCas9) fused to APEX2 is expressed from...

  2. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...). Reprogramming site-specific retrotransposon activity to new DNA sites. Nature. doi: https://doi.org....eB capsid, to express either the optogenetic activator channelrhodopsin-2 or fluorescent reporter dTomato...variants as iGluSnFR4f (fast deactivation) and iGluSnFR4s (slow deactivation). Both have significantly improved...indicators with increased sensitivity and tailored deactivation rates Addgene's own empty lentiviral backbones...indicators with increased sensitivity and tailored deactivation rates By Mike LacyGlutamate is one of the most...indicators with increased sensitivity and tailored deactivation rates. bioRxiv, 2025.03.20.643984. https://doi.org...proteoglycans composed of a protein core, 2–4 glycosaminoglycans (GAG), and are (typically) tethered to the...

  3. Antibodies 101: ChIP

    Type
    Blog Post
    ...downstream analysis focuses on the DNA your protein is actively interacting with, reducing background information...you’re working with a tissue sample, you’ll need to homogenize your sample before moving on to the next...Homogenization for a tissue sample can also need significant optimization for the sample type and size.) It can...process is very similar for both, though tissue samples do require an extra homogenization step. For this...to focus on the ChIP process and not the upsteam sample collection process or the downstream analysis process...itself begins at...  Crosslinking Once you have your sample, the first step is to ensure your protein:DNA complexes...likely to remain attached to the DNA throughout the sample processing and therefore will require crosslinking...

  6. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ... influence receptor conformation and signaling activity​​ (Sridharan et al., 2014). It's also crucial ...addition of fluorophores affects the functional activity of the ligands. For instance, studies have shown...cytoplasmic background signals when used with the same protein (Hayashi-Takanaka et al., 2014). Other dyes....org/10.1038/s41592-021-01338-6 Grimm, J. B., Muthusamy, A. K., Liang, Y., Brown, T. A., Lemon, W. C.,...

  7. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...knockout or edit. This can help reduce off-target activity. Deleting a large region of the genome by removing...transcriptional repressor), and dCas9-VP64 (transcriptional activator)-containing plasmids. Each destination vector ...multiplexed gRNAs? By expressing multiple gRNAs on the same plasmid, you’ll make sure that each cell that gets...situation: you only need to express two gRNAs at the same time. One system you could use is pX333 from the...options that are available from Addgene follow these same assembly principles, but they’re optimized for different...

  8. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    ...gene’s open reading frame, in fact, they can be activated a considerable distance away from a gene which...which GFP was monitoring was being expressed in the same pattern. These systems suggest the type of phenotype...

  11. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ...from AAV2 because they demonstrate high promoter activity (Earley et al. 2020), but non-AAV2 ITRs can be...cis-acting elements, which means that they are in the same DNA molecule as the gene that they package. In a.... M., Dobbins, A. L., Li, C., Hirsch, M. L., & Samulski, R. J. (2020). Adeno-Associated Virus Serotype-Specific...

  12. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...non-small cell lung cancer cell line containing an activating mutation in epidermal growth factor receptor ...replicates would contain the same labels - i.e. they would always come from the same parent cells that represent...replicate groups, and treated these groups with the same drug regimen, they would find one of two outcomes...population (all barcodes can be amplified using the same sets of forward and reverse primers). The abundance...

  13. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    ...gene, as well as to study the effects of inactivating/activating mutations. In biomedical research, modeling...insertions, deletions, and substitutions - at the same efficiency as Gibson Assembly and GeneArt cloning...plasmid. For insertions, it’s possible to use the same technique just using a large primer known as a megaprimer...

  14. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ...human pluripotent stem cells: a transcription activator-like effector nuclease (TALEN) kit consisting ...of individuals and find several mutations in the same gene or in multiple genes, all of which seem to ...insert all of those variants into stem cells with the same genetic background and do an apples-to-apples comparison...

  15. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    ... expertise, publications, and plan for future activities; including the interest of the greater scientific...least 3 full-time people engaged in researcher activities. The EB-1B requires that you provide two out ...working in October of the same year. The Industry H-1B visa is not the same as an academic H-1B visa and...journal editors to request to be a reviewer. Proactivity is very important in this case.  You can still...individually, as a judge of the work of others in the same or allied academic field Evidence of original scientific... prestige of the employer makes EB-1Bs a very attractive option.    Your Expert Letters of support can...

  16. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    ...Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation." Nature biotechnology...has been surrounded by a whirlwind of research activity and development since its inaugural year in 2012...that the CRISPR system was involved. Today, that same laboratory enjoys a successful edit rate of over...later it became quite clear that others thought the same. Since January 2013 there have been 33 CRISPR software... “HTML”, “visual track”, “plot”, “tabular”, “interactive”, and “save”. Trends: Most tools provide tabular...knowledge from laboratories employing CRISPR on the same organism could be used as a proxy for this last ...

  17. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ...and more productive. Higher Productivity. Taking active measures in improving cross team communication ... and engineers were attending the same meetings and hearing the same words, they were leaving these meetings...aforementioned lab manager, said the word “sample,” did the word convey the same concept to Morgan, our senior engineer...help get everyone on the same page. Others in the meeting might also have the same question and asking can...When everyone at a meeting is using the same words with the same meanings consistently, meetings will be...counsel, used the word “service,” did he mean it the same way as Bryan, our customer support manager? When... an Addgene-specific definition. Words such as “sample,” “material,” “item,” and “format” all received...

  18. Antibodies 101: Isotypes

    Type
    Blog Post
    ...antibodies, although not all isotypes have the same ability to activate each of these functions: Recruitment ...appropriate isotype control. An isotype control has the same constant heavy chain domain as your primary antibody...results in the B cell expressing an antibody with the same variable domain but a different heavy-chain constant...

  19. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    ...with a little mutagenic assistance). Quicker CAST activity allowed certain mutated phages to outcompete their...they met the directed evolution expertise of the Samuel Sternberg and David Liu labs. Evolving CASTs for...

  20. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    ...scientific publishing process. There is a rich and active community of science communication professionals...aspects of bioscience into context, while at the same time working through the details of experimental...
Showing: 201 - 220 of 353 results