...knockout or edit. This can help reduce off-target activity. Deleting a large region of the genome by removing...transcriptional repressor), and dCas9-VP64 (transcriptional activator)-containing plasmids. Each destination vector ...multiplexed gRNAs? By expressing multiple gRNAs on the same plasmid, you’ll make sure that each cell that gets...situation: you only need to express two gRNAs at the same time. One system you could use is pX333 from the...options that are available from Addgene follow these same assembly principles, but they’re optimized for different...

...gene’s open reading frame, in fact, they can be activated a considerable distance away from a gene which...which GFP was monitoring was being expressed in the same pattern. These systems suggest the type of phenotype...

...classroom has many benefits. Engaging students in active learning through hands-on experiments allows them...in Columbus, Ohio, ABRC ships more than 100,000 samples to researchers and educators in 60 countries each...

...basal conditions normally after periods of high activity. The new work will help them to understand how...we are working on several genetic diseases at the same time. The limitation of budget restrained us from...

...non-small cell lung cancer cell line containing an activating mutation in epidermal growth factor receptor ...replicates would contain the same labels - i.e. they would always come from the same parent cells that represent...replicate groups, and treated these groups with the same drug regimen, they would find one of two outcomes...population (all barcodes can be amplified using the same sets of forward and reverse primers). The abundance...

...from AAV2 because they demonstrate high promoter activity (Earley et al. 2020), but non-AAV2 ITRs can be...cis-acting elements, which means that they are in the same DNA molecule as the gene that they package. In a.... M., Dobbins, A. L., Li, C., Hirsch, M. L., & Samulski, R. J. (2020). Adeno-Associated Virus Serotype-Specific...

...gene, as well as to study the effects of inactivating/activating mutations. In biomedical research, modeling...insertions, deletions, and substitutions - at the same efficiency as Gibson Assembly and GeneArt cloning...plasmid. For insertions, it’s possible to use the same technique just using a large primer known as a megaprimer...

...human pluripotent stem cells: a transcription activator-like effector nuclease (TALEN) kit consisting ...of individuals and find several mutations in the same gene or in multiple genes, all of which seem to ...insert all of those variants into stem cells with the same genetic background and do an apples-to-apples comparison...

... expertise, publications, and plan for future activities; including the interest of the greater scientific...least 3 full-time people engaged in researcher activities. The EB-1B requires that you provide two out ...working in October of the same year. The Industry H-1B visa is not the same as an academic H-1B visa and...journal editors to request to be a reviewer. Proactivity is very important in this case.  You can still...individually, as a judge of the work of others in the same or allied academic field Evidence of original scientific... prestige of the employer makes EB-1Bs a very attractive option.    Your Expert Letters of support can...

...Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation." Nature biotechnology...has been surrounded by a whirlwind of research activity and development since its inaugural year in 2012...that the CRISPR system was involved. Today, that same laboratory enjoys a successful edit rate of over...later it became quite clear that others thought the same. Since January 2013 there have been 33 CRISPR software... “HTML”, “visual track”, “plot”, “tabular”, “interactive”, and “save”. Trends: Most tools provide tabular...knowledge from laboratories employing CRISPR on the same organism could be used as a proxy for this last ...

...and more productive. Higher Productivity. Taking active measures in improving cross team communication ... and engineers were attending the same meetings and hearing the same words, they were leaving these meetings...aforementioned lab manager, said the word “sample,” did the word convey the same concept to Morgan, our senior engineer...help get everyone on the same page. Others in the meeting might also have the same question and asking can...When everyone at a meeting is using the same words with the same meanings consistently, meetings will be...counsel, used the word “service,” did he mean it the same way as Bryan, our customer support manager? When... an Addgene-specific definition. Words such as “sample,” “material,” “item,” and “format” all received...

...antibodies, although not all isotypes have the same ability to activate each of these functions: Recruitment ...appropriate isotype control. An isotype control has the same constant heavy chain domain as your primary antibody...results in the B cell expressing an antibody with the same variable domain but a different heavy-chain constant...

...from “TGA” to “TGG.”  After identification of ABE activity, XMAS-TREE can also be used to purify these modifications...found in the metagenome datasets from hypersaline samples, were engineered and adapted for RNAi experiments...

... quantitative assays such as antibody specific activity for each reagent batch. To supplement this, Addgene...blog post. Hello, NABOR! Addgene will bring the same open-access benefits to antibodies that you have...

...also observed reliable optically evoked neuron activity for at least several months after implantation...interact during various behaviors. Either tissue samples were harvested after the behavior, or the animals...

...scientific publishing process. There is a rich and active community of science communication professionals...aspects of bioscience into context, while at the same time working through the details of experimental...

...untagged control could enter cultured cells and activate expression of a luciferase reporter. CPP was not... better than immortalized lines derived from the same cell type; however, their usage has been limited...

...dead Cas9 protein (dCas9) that lacks endonuclease activity coupled with a customizable single guide RNA (...The multiple scRNAs all individually utilize the same dCas9 protein. By using an inducible promoter to...

...product, there can be lot-to-lot variability in activity. No Genetic Tagging - Since antibodies are produced...produce the same signal, so only one HRP-conjugated RANbody can be used at a time. Samples can be sequentially...fluorescently labeled tyramide molecule into a highly reactive radical, which is then covalently linked to residues... lower background and better penetration of the sample due to the smaller size of RANbodies compared to...sequentially stained with two HRP RANbodies if the sample is stripped. HRP-RANbodies are, however, compatible...it is approximately as sensitive as detecting the same antigen with a standard primary/labeled secondary...staining of tissue because they have limited cross-reactivity with commonly used anti-rodent or -rabbit secondary...

...HRP, a chemiluminescent conjugate that can be activated to emit light with a simple kit, and is readily... the antibodies time-sensitive, using precious samples, or being compared to other data in a manner that..., simple to use, not sensitive to light in its inactive state, and cheap. Unless there’s a specific reason...signaling molecule, that all need to be detected in the same assay. This is one of the most complex subjects ...