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Addgene
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  1. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    ...fun together! Laura’s Top 10 Event Planning Tips! 1. Take suggestions Some of Addgene’s best events have...culture At Addgene we organically developed the philosophy of  “work hard, play hard.” Part of the reason...
  2. The Challenges of Cell Culture

    Type
    Blog Post
    ...testing. You can find Nick on LinkedIn. References 1. Skloot, Rebecca, and Bahni Turpin. The immortal life...whether the cell lines had isoenzymes and genetic polymorphisms specific to their origins. This technique is...
  3. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...SpCas9 base editors with editing windows as small as 1-2 nucleotides! To reduce off-target effects associated...Lower on-target activity than BE3; editing window ~1-2 nt  87438  pET42b-HF-BE3  HF-Cas9 (NGG)  Very ...compatible with two-photon microscopy using Ti-Sapphire lasers. pBAD-RDSmCherry1 is a red-shifted variant...
  4. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...indel formation and phenotypic changes as early as 1 week post-injection. Livers from these mice were histologically... use of St1Cas9 (~3.3 kb) from Streptococcus thermophilus and a rationally-designed truncated Cas9. Unfortunately...
Showing: 201 - 207 of 207 results