Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR
Type
Blog Post
... in proximity by the looping of chromatin, the complex of DNA and proteins that forms chromosomes.
These...of a stable cell line that co-expresses three components:
dCas9 with an added biotin acceptor site
BirA...genomic location of interest
When these three components are co-expressed, the gRNA targets the dCas9 ... of the CAPTURE method. After protein and DNA complexes are cross-linked, DNA is digested into smaller.... This is cumbersome and also makes it hard to compare results across multiple targets because different...with primary cells or rare cell populations.
Key components of the CAPTURE 2.0 system
To improve upon the... Using this tag reduces the number of CAPTURE components that need to be delivered to cells from three...