...because it required only about 15 minutes of my hands on time and a total of about 3 hours. It was robust...Addgene lab. In this post, I’ll tell you a little bit about what I learned from a New England Biolabs (NEB) ...this, but thought we could learn a little bit more about high throughput DNA prepping by trying out NEB’s... new teaching space at Lab Central to learn all about it. LabCentral is a lab and office space shared ...without breaking their bank accounts. When I heard about how LabCentral aims to enable emerging biotech companies...different biotech companies to interact and learn more about one another. They also provided both breakfast, ...go to a workshop because it will help you think about the most useful pieces of information from the workshop...

...and Jeffrey Tabor have been working on making E. coli light responsive since 2005, when Tabor was working...these classic systems continue to be optimized by Tabor’s lab, making light-controlled gene expression in...for the computational problem of edge detection. Tabor et al. subsequently engineered a second photosensitive... and highest level of “output” signal, was only about 10-fold, precluding the study of gene expression...-component systems. Schmidl SR, Sheth RU, Wu A, Tabor JJ. ACS Synth Biol. 2014 Nov 21;3(11):820-31. doi...Escherichia coli to see light. Levskaya A, Chevalier AA, Tabor JJ, Simpson ZB, Lavery LA, Levy M, Davidson EA, ...Addgene. A synthetic genetic edge detection program. Tabor JJ, Salis HM, Simpson ZB, Chevalier AA, Levskaya...

... advantage. It wasn’t until I wrote a blog post about a research paper that explored how scientists connect...saw a request for an Addgene guest blogger post about colony PCR, I signed up immediately. I had used ...science communication skills, while also learning about new scientific developments and helping scientists... found this paper. I wrote an Addgene blog post about it and shared the paper with my lab since one of...project. I was invited to participate on a panel about “Social Media for Science Communication” at the ...Twitter pros as well, like tip #4: “Follow people for about a week and then unfollow those who do not capture...social media to consistently engage with others about your topic(s) of interest. Hopefully through these...

...Ultimately, problem solving is less about technical know-how and more about how you approach new challenges...transferable skills guide: Problem-Solving. I don’t know about your experience in the lab, but mine was plagued...the job description, but this skill is not just about reacting to technical issues. This skill also comes...I try to clarify what people mean when they ask about “problem solving skills” and look at how scientists...effectively resolves the issue.  If this sounds like about seven different skills to you, you are not wrong...Imagine your problem is just a question you have about the world. You might do some background research...went through your head as you made observations about the issue, assessed potential protocol modifications...

...Northeastern University, she interviewed Addgenies about their career path. Graduate school: Do I or ...or don’t I? I asked the nine Addgenies about why they did or did not go to grad school. QC Scientist Alyssa...our Financial Operations Specialist, Lucy Hammer, about why she did not go. She told me, “I think that my...Perkins noted that there is a more emphasis today about non-academia and industry jobs. Since there are ...to keep my values in mind as I applied. Thinking about this made me curious. I wondered what my coworkers...view of science. It should be really open and collaborative.” She knew she wanted to leave academia, and...from my co-op. It has been so insightful to learn about different career paths, successes and failures, ...

...School visited our labs in Cambridge, MA to learn about the wide variety of science careers that Addgenies...s never too early to start networking! Learning about careers at Addgene Afterwards, the students split... was very cool!” and Izzy Shaw, who was excited about “Seeing a real lab!” And several of our visitors...visitors made sure to tell us what we already knew about our Shipping Operations Coordinator: “Kory was awesome...awesome!”. The students also got to learn about intellectual property from our Tech Transfer team members...are offered with a biology major.” After hearing about many Addgenies’ career paths, Michelle Healy said...work to help improve K-12 education? Let us know about your educational events in the comments section ...

...carrying a synthetic gene drive were to escape the laboratory, the drive could eventually spread through the...mistake would necessarily extend far beyond the laboratory and seriously damage public trust in scientists... can spread through populations of transgenic laboratory organisms but not wild organisms. For example...synthetic sequence will only spread in transgenic laboratory populations with that sequence2. Both methods...Reproductive confinement involves working with laboratory organisms that can't reproduce with wild ones...confinement seeks to keep the organisms in the laboratory. It varies by organism, but your local biosafety...population and immunizes it against the first drive. Laboratories interested in building candidate gene drives...

...When you think about going to a scientific conference, you may think about sitting amongst a sea of chairs...After learning about using sound to visualize bacteria in the lab, I was excited to learn about creative approaches... at different ultrasound frequencies. Read more about ‘Using ultrasound to image bacteria in vivo: acoustic...Protein Resources and get started!  I learned a lot about creative ways to do science communication and outreach...outreach - if you'd like to learn more about Science communication check out these SciComm blog posts.... Methods for Bacterial Genome Engineering Learn about Science Communication using Twitter  Resources ...

...scientists to make genomic alterations, bringing about a revolution in genome engineering, with new techniques...available by both the Jacob Corn and Alex Schier laboratories. Delivering Cas9-gRNA ribonucleoproteins  An...delivery of RNPs is electroporation (A in the figure above), which generates pores in the cell membrane, allowing...as lipid-mediated transfection (B in the figure above), are in the early stages of development. David ...proteins harboring receptor ligands (C in the figure above), which result in the internalization of Cas9-gRNA...show promise for in vivo studies, little is known about the immunogenicity and stability of the Cas9 protein...and purification, which will require additional laboratory reagents, equipment, and expertise. Final thoughts...

...didn’t have to worry about having to hunt down sources later on.  A comment about writing: initially I...exists. Once you pick a topic, try to be specific about exactly what aspect of the field you plan to review...themes in the field and to learn what people cared about. I also used reviews to get a list of research papers...highlighted a few dozen things that I didn’t know about. Instead of reading a paper and getting my bearings...them. No really, just start writing! Don’t worry about grammar or formatting or continuity. Also don’t ...worry if you feel like you don’t still know enough about the topic. Just get as many words down on the page...between office hours and experiments. A comment about citing: I wasn’t sure if I should cite reviews or...

...The Addgene-Seeding Labs plasmid grant is a collaborative endeavor providing plasmids to researchers in... of Zimbabwe, Zimbabwe Dr. Idah Sithole-Niang (above, left) is an Full Professor in Biochemistry with... CRISPR effect observed in bacteria. Learn more about the different CRISPR applications in plants.     ...Rodrigo Arturo Rivera Solís, Associate Professor (above, far right), thrives to make affordable biosensors...detect glyphosate via H2O2, one of the reaction’s metabolites. One useful genetically encoded H2O2 probe is... to the Addgene blog for updates and future collaborations. Many thanks to all of the awardees, all who...Additional Resources on the Addgene Blog Learn More About the Addgene-Seeding Labs Plasmid Grant Check Out...

... will also serve as a good mentor. To read more about what makes a good mentor, see the previous post ... and personnel management?  If you are thinking about a non-academic path, will the PI support this path...Medical School researcher Connie Cepko to learn about her mentoring style. How can I find out if an advisor...time with the potential advisor and don’t be shy about asking hard questions. Talk to as many lab members... past lab members. Most scientists love to talk about their past lab experiences and will be willing to...members, neighbors or alumni give you information about the PI that concerns you, don’t just brush this ...Mentors by @drmellivora, an excellent blog post about workplace bullying featured on the always interesting...

...Cell tracing in zebrafish with Zebrabow Before the generation of Zebrabow (Pan et al., 2011), Brainbow ...crossing of Zebrabow animals to an inducible Cre line. The Schier group developed Zebrabow as a system...by injecting Brainbow at the single-cell stage. Zebrabow, or “zebrafish brainbow,” is a collection of transgenic...are introduced using a plasmid construct called Zebrabow-GateDest containing an RFP, CFP, and YFP downstream...downstream of a ubiquitous promoter. Zebrabow is an extremely versatile technology as recombination can be...an introduction to live cell imaging Learn more about the origin of the Brainbow system Visit our fluorescent... Addgene’s fluorescent protein collection Learn about fluorescent tools for in vivo imaging ...

...a successful negotiation and collaboration. We’d love to hear more about your experiences and opinions...can get away with using computer modeling and collaborations rather than using animals, then that’s a cost...associated with old equipment, like service repairs, labour costs, and loss of time, can exceed the cost of...Everything is negotiable. Try asking for discounts, collaborations, access to internal award applications, etc...This is a non-profit organization. Therefore, collaboration and community is the ultimate value proposition...Happy Sciencing, Damien   Damien Wilpitz is a laboratory research management consultant and coach in Boston...research scientists create productive and manageable laboratory environments. His business skills enhance his...

...the Addgene Blog Learn about Efforts to Fight Rare disease with iPSCs Read About Addgene's Outreach Efforts...the first clinical trial with iPSCs with his collaborator Takahashi in 2014 – just 8 years after the OSKM...researchers special: 1. Stem cell scientists care deeply about ethics Ethics must play a prominent role in the ...Researcher Darrell Kotton 2. Stem cell scientists care about patients As any grad student who has spent years... a big picture perspective: Dr. Yamanaka talked about his clinical trial patients with advanced macular...proof that these researchers are thriving in a collaborative, rather than a combative, field. Of course they... in the Microbiology Community Sign Up to Write About Stem Cells Resources on the Addgene Wesbite Find...

...communication in remote collaborations Transferable Skills Guide: External collaborations Transferable Skills... stint may be ideal for you. Here, we will chat about who might want to explore this role, how to seek...scientist is distinct from equipment sharing or a collaboration – it requires re-location of the scientist to...reasons Visiting gives you access to possible new collaborations and fresh perspectives. For trainee level researchers...future work placements. The chance to learn and collaborate all while experiencing a new city or maybe even...there is someone you personally know or your lab collaborates with that can fulfill your research needs, that... open to sharing knowledge and establishing collaborations, so it doesn’t hurt to reach out and ask if...

...fully-equipped laboratory for citizen scientists. In addition to maintaining a laboratory to support both... of independent Do-it-Yourself (DIY) biology laboratories working towards enabling everyone to participate...Anyone can become a member of BosLab and previous laboratory experience is not required. Our members are skilled...have non-traditional scientists working in our laboratory! Don’t worry though, we make sure that everyone...samples. The goal is to not only learn something about the microbial diversity in different soil conditions...community lab in a city near you. To learn more about what we’re up to or how you can help BosLab, visit...a co-organizer at BosLab, a community biotech laboratory in Somerville MA. Follow her on twitter at @ZebraElement...

... communication, and in particular communication about experimental methods and approaches. It’s very easy...everything will be fine. It won’t. When we hear about issues with reproducibility, time and time again...the problem comes down to inadequate information about what the original researchers actually did. Admittedly...Communication comes in many forms—from transparency about data to allow others to see what you actually found...fits together to make for a transparent and collaborative research environment. Journals as enforcers ...of methods sections, including clear information about reagents, and why other publishers and organizations... in reproducibility jail and more effective collaboration to drive the progress of science. Sounds like...

...see the new map in action). Read on to learn more about the improvements we’ve implemented and how they’...more functional display, you can start thinking about your next cloning experiment early on. Beyond these... to to download the static image file (PNG, see above) for your own reference or to paste directly into...identified a plasmid that you want to learn more about, updates to our sequence analyzer will allow you...the features shown in the static maps discussed above, but also have interactive functionalities. You ...enzyme, primer, or ORF to learn more information about it. For example, if you hover over the ampicillin...guide A quick guide to all of the tabs listed above and their functions. We hope that these updates...

...designed (listed above) worked well and I got clear bands using the conditions above. 2. Design your primers...essential for experiments but that nobody talks about. After months of troubleshooting, those people who...details. The entire process from start to finish took about 1 month. Cas9 delivery Overview: Before any ...is a loading control. Pro-Tips: 1. It’s all about the MOI. In retrospect I used a way too high Cas9...original publication they see GFP expression in about 20% of their Cas9-expressing cells. So, even when... gene has a weird mutation that you didn’t know about (which could affect the ability of the gRNA to bind...engineering has come. For comparison, in 2007 it took about 3 months to knockout a single allele in a human ...