We narrowed to 1,073 results for: TOR

... Depositor Collections Botman-Teusink Yeast FP Collection...Botman et al., 2019 . FP Tagging Yeast tagging vectors to create fusions of proteins of interest with ...yeast codon-optimized fluorescent proteins. These vectors are based on the pFA6a-link plasmid (Sheff et al...overexpression of codon-optimized FPs in yeast. Vectors are based on the pDRF1 plasmid (Loqué et al., 2007...blue, green, and red fluorescent protein tagging vectors for S. cerevisiae. PLoS One, 8 (7):e67902. https...Nielsen, L. K. (2013). Dual gene expression cassette vectors with antibiotic selection markers for engineering...

...Neuroligin (Link opens in a new window) receptor families, key mediators of synaptic adhesion and function....recombinant antibodies targeting axonal guidance receptors and ligands (glypicans and integrins), components...development. Figure 1: Key adhesion proteins and receptor–ligand pairs bridge the presynaptic and postsynaptic...Integrins are a family of mechanotransduction receptors, comprised of alpha-beta subunit heterodimers....specific subunit pairs, blocking ligand binding and receptor function Figure 3: IPI integrin antibodies exhibit...

...plasmids in the repository. Browse our AAV collection of site specific recombinases.... Viral Vector Packaging ...Packaging Service AAV Recombinases Viral Vector Packaging Service: Recombinases Cre AAV Dre AAV Flpo AAV Light-Inducible...Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . Don’t See What You’re Looking For? Our ... eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know...format. Please note this does not guarantee viral vector packaging service, but lets us know what viruses...

... Depositor Collections New England Biolabs Cell-Imaging Plasmid...Plasmid Substrate Feature 101137 pSNAP-tag (T7) Vector SNAP-tag Empty backbone for bacterial expression...expression under T7 control 101135 pSNAP-tag (m) Vector SNAP-tag Empty backbone for stable and transient mammalian... cell surface localization 101136 pCLIP-tag (m) Vector CLIP-tag Empty backbone for stable and transient...for cell surface localization 101127 pMCP-tag (m) Vector ACP/MCP-tag Empty backbone for transient mammalian...mammalian expression 101126 pACP-tag (m)- 2 Vector ACP/MCP-tag Empty backbone for stable and transient mammalian...

... Resources Gamma-Retroviral Vector Guide All Viral Plasmids Viral Vectors 101 eBook Biosafety Resource...gamma-retroviruses have been engineered into gamma-retroviral vectors for safe use in the lab. The gamma-retrovirus components...and envelope plasmids. Read our Gamma-retroviral Vector Guide for more information about gamma-retroviral... production, and viral safety. Gamma-Retroviral Vector Guide Popular Gamma-Retroviral Transfer Plasmids...for the addition of WRE, a post-transcriptional regulatory element that increases export to the cytoplasm...

... within that gene. To construct ZFN expression vectors, DNA encoding zinc finger arrays should be cloned...plasmids also available through Addgene--the specific vector to use depends on the length of the spacer sequence...between the half-sites (see Nuclease Expression Vectors for more details). IMPORTANT NOTE: All zinc finger...tCCCATCCTAtcgttGCATGAGCTc mdka (midkine-related growth factor) OZ527 and OZ528 OPEN OPEN cACCCTCCTCgtggtGCTGGTGGCc...aAGCTTCCTCttcattgGCTGACTGCa ldr (low density lipoprotein receptor) OZ539 and OZ540 OPEN OPEN tTGCATCCACggcagTCGGCAGTGc...OPEN OPEN gAGCTCCGTCtcgctgGCGGCCGAAg histamine receptor H3 (hrh3) OZ549 and OZ550 OPEN OPEN gCGCCACGGAcaataaaGAGGACTGCa...

...signed by an authorized signatory at the recipient institution. An authorized signatory is someone who can ...also adding additional filters such as expression, vector type, species, and more. Plasmids Browse the table...Golden Gate Assembly Standards Kit Cloning Eric Cantor Kit for using comprehensive end-joining ligation...Libraries CRISPR Thorsten Stiewe Use these donor vector libraries for CRISPR/Cas9-mediated saturation genome...entity is qualified to receive materials from our repository. All purchases are subject to Addgene's Terms...

...General Tools Fire Lab C. elegans Vector Kit - Andrew Fire Lab. A set of vectors for C. elegans research, including...plasmid tools for the community that aren't in the repository? Help us widen our resources for the community...plasmids that streamlines the production of targeting vectors for tag insertion using CRISPR/Cas9 and the selection... approach has also been developed by the Fire laboratory and described in Efficient Marker-Free Recovery...including lacZ and GFP fusion vectors. C. elegans optimized fluorophores - Andrew Fire Lab. Available ...

...References The ARF (ADP-ribosylation factor) family of regulatory GTPases have long been characterized...exchange factors), and downstream (by GAPs, GTPase activating proteins, which also serve effector roles)...ARF family plasmids. ARF family members include regulatory GTPases, GEFs, and GAPs. Plasmid..., 2016). Though best known for their roles as regulators of membrane traffic, they also play critical ...

...Examples Include: promoters and terminators repressors and activators logic gates Networks and Gene Regulation...plasmids available from this depositor's lab. Plasmid Gene/Insert Vector Type Promoter PI Publication ...includes both naturally ocurring and synthetic regulatory elements, pre-assembled genetic circuits, and...

...plasmids to study the regulatory networks involved in retinal development.... Depositor Collections Brzezinski...mouse retina. To aide in their studies of the regulatory networks involved in retinal development the ...Prdm1 and Vsx2 enhancers in the retina alters photoreceptor and bipolar cell fate specification, yet differs...

... SLCs in Gateway-compatible pENTR vectors (pDONR221). These vectors allow a fast and efficient sub-cloning...framework that provides biological context. Assays monitoring impedance, fluorescence, mass/charge properties... into a wide range of Gateway-compatible pDEST vectors. ID Plasmid Gene/Insert Industry PI Pooled Libraries...the Resolute Partnership using their RESOLUTE repository feedback form (Link opens in a new window) . ...

...your experiment using the lentiviral vector system. Viral vectors have been increasingly popular in fundamental...primary cells. Amongst the different vectors used, lentiviral vector constructs have proven very useful ...fluorescent sensors. Retroviral vector background Until the development of viral vectors, gene transfer into mammalian...un-transfectable cells, labs use viral vectors and particularly retroviral vectors because they target a broader...attractive choice for a gene transfer vector. Lentiviral vectors Although retroviruses are still in use...generation of lentiviral vectors consisted of three plasmids encoding 1) the lentiviral vector genome which was...lentiviral vector system offers the best safety profile in terms of RCL generation because this vector requires...

...First, some history. In 2008, the Auricchio lab decided to solve the issue of “tiny vector, big transgene... overviews of three split vector strategies plus one additional split vector strategy for increasing the...homology directed repairs (HDR). Split AAV vector approaches Split vector approaches increase the size of the...copies of both vectors. Examples of use: Halbert et al., 2002 used an overlapping vector with 440 or 1000bp...splice site donor and acceptor sequences and the homologous region of the two vectors can be from one of ... hybrid vector, as measured by β-galactosidase activity, was 81% of that from a single vector, but significantly...ideal split vector system. Overall the efficiency of transgene expression from split vectors has been inconsistent...

... the perfect voltage indicator and optogenetic actuator pair. Ideally, indicators should possess fast ...the voltage indicator, QuasAr, and actuator, CheRiff, in the bicistronic Optopatch vector, the Cohen lab... our community of depositors. With over 40,000 plasmids avaliable in the repository, we can't give all...University of Wellington, Victoria, has developed an adaptable bacterial expression vector which gives close ...science.aaa3380 MuLE vectors for customized lentivirus production Finding a lentiviral vector backbone with ...custom-cloned vectors in a Multisite Gateway reaction with a desired destination vector expressing a separate...a final lentiviral vector containing up to 5 different elements. Lentiviral vectors constructed with the...

... Depositor Collections FreeGenes Project Open Enzyme Collection... own enzymes by cloning them into an expression vector of their choice and transforming into E. coli bacteria...Researchers should clone these enzymes into an expression vector of their choice and then transform into E. coli...expression and purification ( pET28a-LIC or other pET vectors are recommended). The selection marker for all ... top Additional Resources Bacterial Expression Vectors Synthetic Biology Plasmids MoClo Guide Drew Endy...

...genes, the laboratory of Jason Moffat at the University of Toronto has created the Toronto KnockOut (TKO...both laboratory and prototrophic strains. The vectors were based on the original EasyClone vectors (Jensen...coupled receptors (GPCRs) yet one third of the GPCRs in the human genome are orphan receptors meaning ...tools, compounds, and assays to monitor activation of these receptors. Bryan Roth's lab has a newly available...expression vectors with various promoters suitable for in vivo expression, and/or produce vectors to make... all TAL effector scaffolds previously constructed for the Golden Gate TALEN and TAL Effector Kit 2.0.... These vectors contain uracil-excision based (USER) sites, flanked by ADH1 and CYC terminators for easy...

...Updated mini-transposon vector for bacterial mutagenesis or gene targeting Victor de Lorenzo's lab has... existing transposon and vector systems and creating an all-synthetic vector that included only the elements...features to be monitored to study mitochondrial dysfunction. Easy-to-use tools to monitor these features... into a destination vector in a predefined order. Finally, the destination vectors are used with Multisite... eight entry vectors can be used for each Multisite Gateway compatible destination vector for a maximum...optogenetics tools – light-activated receptor tyrosine kinases Receptor tyrosine kinases (RTKs) are a large...step, compatible Level 0 vectors are directionally assembled into a Level 1 vector creating a single transcriptional...

...polycistronic vector or multiple single-gene vectors can be used to package reprogramming factors. An important... OSKM reprogramming factors: Mouse Expression Vectors Human Expression Vectors Carey et al Hockemeyer...reprogramming vectors available from Addgene. Another drawback is that lentiviral vectors insert in different...Cre-lox lentiviral vectors: This approach is the same as using a standard lentiviral vector, but transient...Non-integrating iPSC delivery methods Viral Vectors Adenovirus: Adenoviral vectors infect dividing and nondividing...different adenoviruses, each expressing one factor. These vectors don’t integrate into the genome and are ...mutagenesis, adenoviral vectors are considered a safer way to express reprogramming factors for therapeutic applications...

...building successful mentor relationships, asking for mentor support, and choosing your mentor. What isn’t discussed...receive technical mentorship from a ‘bench mentor’. In some instances, the bench mentor is also the scientific...Scientist Edition Mentoring for Scientists: I Have a Mentor, What Now? Will You Be My Mentor? Finding and ...often enough is the hands on mentoring you receive after you’ve found your mentor, though. Typically, you ...scientific mentor…but usually the bench mentor is a staff scientist, postdoc, lab manager, technician, or...more, depending on the mentoring style within the research group). The bench mentor-mentee relationship ...lot more face time with your bench mentor than your scientific mentor. Thus, this relationship will probably...