...usage bias in Plasmids 101: Codon usage bias Read more about plasmids in our Plasmids 101 blog series Learn...Reference Read about genetic code expansion Browse our plasmid collection page  ... in Biotechnology 22:346–353 . https://doi.org/10.1016/j.tibtech.2004.04.006 Hausser J, Syed AP, Bilen.... Genome Research 23:604–615 . https://doi.org/10.1101/gr.139758.112 Mauro VP, Chappell SA (2014) A critical...Molecular Medicine 20:604–613  https://doi.org/10.1016/j.molmed.2014.09.003 Nakamura Y (2000) Codon usage...Neuroscience Methods 124:75–81 . https://doi.org/10.1016/s0165-0270(02)00362-x Stadler M, Fire A (2011) ...USA 107:3645–3650 https://doi.org/10.1073/pnas.0909910107 Varani G, McClain WH (2000) The G·U wobble base...

... kit Read our blog posts about plasmid cloning Browse our Plasmids 101 blog posts Resources on Addgene.org...fragmenting plasmid DNA, PCR product, or chromosome. One of the main causes of degradation during plasmid DNA ...Biology Reference Find protocols on plasmid cloning Find plasmids for your research ...Nowadays, the most common E. coli strains used for plasmid amplification are endA negative, meaning the gene...degradation and which experiments you can use your plasmid.  DNA elution choices: TE, Tris buffer, or water...polymerases. In the situation where you need to use plasmid in TE for any enzymatic reaction, an effective ...choices that companies offer for the elution of plasmid DNA through their kit. The advantage of using a...

...AAV) Plasmids Additional Resources: AAV Guide Biosafety Guide All Viral Plasmids Blog: Plasmids 101-Viral...AAV use in CRISPR Viral Vectors 101 eBook Packaging Plasmids AAV Plasmids Adeno-associated viruses (AAV)...with the plasmid that encodes the viral genome (typically called cis -plasmid or transfer plasmid). Helper...Helper Plasmid ID Plasmid Serotype Description PI 112867 pAdDeltaF6 All Helper plasmid for AAV packaging... Viral Plasmids Adeno-associated Virus (AAV) Plasmids Adeno-associated Virus...flexibility. AAV Packaging Plasmids The following tables contain a list of packaging plasmids that are useful to...particles, a helper plasmid (encoding adenovirus E4, E2A and VA) and a RepCap plasmid (encoding AAV Rep ...

...generate stable cell lines (for details, see Plasmids 101: Mammalian Vectors ) Transient or stable expression...Additionally, many plasmids containing validated gRNAs are now available through Addgene. These plasmids contain ...particular model organism. There may not be a perfect plasmid for your specific application, and in such cases...different activators exist, including the multi-plasmid SAM system. Select Expression System To use CRISPR...and enrich positive cells Packaging and envelope plasmids provide the necessary components to make lentiviral...Read more about how to design your gRNA . Browse Plasmids: gRNAs Synthesize and clone desired gRNAs Once...oligos are synthesized, annealed, and inserted into plasmids containing the gRNA scaffold using standard restriction-ligation...

...Addgene blog Antibodies 101: Monoclonal Antibodies Plasmids 101: Peptide Tagging Plasmid-based Recombinant ... recombinant anti-c-Myc [9E10] at Addgene Find plasmids with the myc-tag  ...binding protein. Cell 46:291–300. https://doi.org/10.1016/0092-8674(86)90746-4 Munro S, Pelham HRB (1987)... ER proteins. Cell 48:899–907. https://doi.org/10.1016/0092-8674(87)90086-9  Schüchner S, Behm C, Mudrak...

...Biosafety Guide Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook γ-Retrovirus (gamma-retrovirus) is an... and Packaging Plasmids Common γ-retroviral envelope and packaging plasmids. ID Plasmid Type Description...envelope plasmid Kasparov Popular γ-Retroviral Transfer Plasmids Common γ-retroviral transfer plasmids. Want...retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids. Viral...Plasmids γ-Retroviral Plasmids γ-Retroviral Plasmids Additional Resources: γ-Retrovirus Guide Biosafety...with packaging plasmid Weinberg 12259 pMD2.G Envelope VSV-G-expressing envelope plasmid Trono 1733 pCI-VSVG...envelope plasmid Nienhuis and Salmon 35616 pCAG-VSVG Envelope VSV-G-expressing envelope plasmid Nienhuis...

...proteins 101 eBook Read our plasmids 101 blog series Resources on Addgene.org Browse our plasmid collection... resistant to chemotherapy. You can find these plasmids at Addgene. Another advantage of mice as model... pancreas. They first tagged these cells using plasmids. Then using HOTcre, a method that uses heat induction... feeding the worm bacteria expressing specific plasmids (L4440) that contain your gene of interest (Fire...calcium signaling in the entire adult brain, using a plasmid that expresses a genetically encoded calcium sensor...al., 2015). If you are interested in C. elegans plasmids and resources check out Addgene’s Worm Expression...pages Read our molecular biology reference Find plasmids for your research ...

...Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection.  mEmerald plasmids and many more...Molecular Expressions collection including some 3,300 plasmids along with image galleries and educational resources...resources to go with them. This collection of plasmids is available in an easily searchable format on Addgene...Fluorescent Protein Collection webpage. These plasmids are divided into the following categories: Over...list of cell markers all tagged with mEmerald. Plasmids to monitor protein-protein interactions through...Specialty constructs for biosensing. Over 300 plasmid backbones spanning a rainbow of fluorescent proteins...proposition, explained Michelle Baird, who has created plasmids as a technician in the Davidson lab for the last...

...GreenGate Plasmids Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More Plasmids 101: Origin...stability of modified Ti plasmids during plant infection, as well as plasmid yield during preparation ...Agrobacterium carries a “tumor-inducing” or Ti plasmid, which allows it to transfer genetic material into...in two species: Agrobacterium and E. coli. Like plasmids used in other systems, binary vectors are cloned...It’s a lot of information to fit into a single plasmid, so most first-generation binary vectors are quite...pSiM24 are suitable for many applications, the plasmid can also be customized due to its modular structure...Addgene Check out Addgene’s plant vectors! Find the plasmids from this paper at Addgene. Other relevant blog...

...Antibodies 101: Introduction to Immunofluorescence Antibodies 101: Epitope Availability Antibodies 101: Introduction...Addgene.org Find recombinant antibodies at Addgene Find plasmids encoding recombinant antibodies at Addgene   ...

... on All Things Plasmid with Our Plasmids 101 Series Get Tips on Verifying Your Plasmid Resources on Addgene.org...Check out Our Plasmid Reference Pages Deposit Your Completed Plasmids Find the Perfect Plasmid for Your Research...recipient plasmid (usually in the Multiple Cloning Site (MCS)), but do not cut elsewhere on the plasmid Bonus...recipient plasmid. Remember to insert your DNA in the correct orientation in the recipient plasmid by viewing... of DNA so that it can be easily cloned into a plasmid of interest. You can use similar processes to add... Interest (YGOI) for ligation into a recipient plasmid. Designing primers for PCR based cloning   ...this example). Assuming you are amplifying from plasmid DNA (rather than from genomic DNA or a cDNA library...

... Download the fluorescent proteins 101 eBook Read our plasmids 101 blog series Resources on Addgene.org...zebrafish CRISPR plasmid collection Read our molecular biology reference Find plasmids for your research...

...because it relies on a single BiFC pair. Find BiFC plasmids for your experiment at Addgene! How to Design ...proteins, check out our article: Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection...straightforward in easy-to-transfect cell lines where plasmid dosage can be tightly controlled. This is also ...the Addgene blog Browse our Fluorescent Protein 101 blog posts Read our blog post covering an introduction...Resources on Addgene.org Find fluorescent protein plasmids and resources Visit our Molecular Biology Reference...Complementation. Molecular Cell 9:789–798. https://doi.org/10.1016/s1097-2765(02)00496-3 Kerppola TK (2006) Design...Harb Protoc 2013:pdb.prot076497. https://doi.org/10.1101/pdb.prot076497 Kim Y, Jung JP, Pack C-G, Huh W-...

...resources on the blog CRISPR 101: Cas9 vs. The Other Cas(s) CRISPR 101: Anti-CRISPR Proteins Switch Off...its partner in crime, Cascade. Find Cascade-Cas3 plasmids in Addgene's collection! A “complex” system Cas3... Various labs have designed all-in-one Cascade plasmids — such as the Tomoji Mashimo lab and the Tilmann...resources on Addgene.org CRISPR guide Cascade-Cas3 Plasmid collection ...Molecular Cell, 70(3), 385-394.e3. https://doi.org/10.1016/j.molcel.2018.03.031 Xiao, Y., Luo, M., Dolan, ...and Genome Editing, 3–4, 100013. https://doi.org/10.1016/j.ggedit.2022.100013 O’Brien, R. E., Bravo, J. ...Molecular Cell, 83(5), 746-758.e5. https://doi.org/10.1016/j.molcel.2023.01.024 Morisaka, H., Yoshimi, K.,...

...constructs from Christopher Harvey’s lab. These plasmids localize opsins to the soma to avoid off target...neighboring neurons. We have also packaged hChR2(H134R) plasmids from Karl Deisseroth’s lab, which includes pAAV-hSyn...H134R)-EYFP that is both Cre and Flp dependent. Plasmid Serotype Name 124603 AAV9 pAAV-EF1a-DIO-ChrimsonR-mRuby2... has now expanded to offer many of our control plasmids in new serotypes. See our Serotype Testing AAV...AAV inventory for the entire collection! Plasmid Serotype Name 51502 AAV5 pCAG-FLEX-EGFP-WPRE 114471... jGCaMP7 from Janelia, including three jGCaMP7 plasmids now packaged in AAVrg. jGCaMP7s, the variant with...post Overview of AAV Encoded Calcium Sensors). Plasmid Serotype Name 104489 AAVrg pGP-AAV-syn-jGCaMP7b-WPRE...

...Vectors 101: Viral Applications Adenoviral Vector Production and Troubleshooting Viral Vectors 101: Types...vectorization strategies include creating an AdV transfer plasmid with the E1 and E3 (Early 1 and Early 3) genes .... These genes’ functions are rescued by helper plasmids used during production and/or by genes present...made available by this deletion from the genomic plasmid can be used to house a recombinant sequence (transgene...late genes are usually present in the transfer plasmid for the majority of adenoviral vector systems used...promoters. Mol Ther, 7(4), 526–534. https:/doi.org/10.1016/S1525-0016(03)00023-6. PMID: 12727116. Borkenhagen...Biomedical Sciences. Elsevier. https://doi.org/10.1016/B978-0-12-801238-3.02523-X. Hofmann, C., Löser,...

...Synthetic Biology Education Nonprofit BioBuilder Plasmids 101: Golden Gate Cloning Learn About Working at ...Michael Koeris Resources at Addgene.org Find Plasmids from the Ellis Lab Find Synthetic Biology Kits...

...viral vectors and plasmids with repeating sequences. Repeating sequences within a plasmid can undergo intramolecular...contain the full length plasmid.   Figure 1: The full lentiviral plasmid is expected to be >12 kb...in my samples? Plasmid recombination can affect viral vectors (LTRs and ITRs), plasmids with multiple ...other plasmids that contain repeating elements. Knowing how to amplify recombination-prone plasmids and ...browse the table of common gene mutations in our Plasmids 101: Common Lab E. coli Strains blog post. Check... length plasmid. If you find that your bacterial cultures contain a mix of full length plasmid and the...smaller, recombined plasmid around 1.5 kb. When cut with BamHI, the expected linear plasmid is present, but...

...Chemogenetics Plasmid Serotype Name 119472  AAV5  AV SYN PSAM4 GlyR IRES EGFP   Biosensors Plasmid Serotype...inventory of serotype testing AAV New control AAVs Plasmid Serotype Name 105530  AAV1 pAAV.CMV.PI.EGFP.WPRE.bGH...biosensor AAV inventory New glutamate sensors Plasmid Serotype Name 106176  AAV1  pAAV.hSynapsin.SF-iGluSnFR.S72A...pAAV.hSynapsin.SF-iGluSnFR.S72A New calcium sensors Plasmid Serotype Name 104496  AAV1 pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE...pAAV-hSynapsin1-FLEx-axon-GCaMP6s New voltage sensors Plasmid Serotype Name 119036  AAV1  pAAV-hsyn-flex-Voltron-ST...of DREADDs / Chemogenetics AAVs New DREADDs Plasmid Serotype Name 75033  AAV1 pAAV CD68-hM4D(Gi)-...pAAV SYN1 HA-hM3D(Gq) New chemogenetics AAV Plasmid Serotype Name 119741  AAV5  AAV SYN flex PSAM4...

... Find plasmids from this publication at Addgene.   Addgene’s Fluorescent Protein Plasmids and Resources...Resources Relevant Blog Posts Plasmids 101: Green Fluorescent Protein Which Fluorescent Protein Should I... Methods. 2009 Feb 6(2):131-3.  PubMed. Find plasmids from this publication at Addgene. Subach OM,...