Ligation Independent Cloning
Type
Protocol
...30 minutes. Inactivate T4 Pol by heating to 75° for 20 minutes. Volume (μl) Reagent Final Conc. 4 10X ...enzyme, and remove the restriction sites from the final product (no "cloning scars"). A “stuffer” sequence...the following T, so that there are no gaps in the final assembly. In this case, 15 bp of homologous sequence...