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Showing: 181 - 200 of 241 results

  1. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...). Created with BioRender.com. Before using gRNAs in vivo, they should be screened for activity in...transcription at genomic sites targeted by specific sgRNAs. Its RNA-guided nature makes CRISPRa much simpler...all the protein and RNA components, and several sgRNAs must be screened to identify effective ones. Simplified...constrained by AAV packaging limits (i.e., how many sgRNA expression cassettes will fit). Cre-dependent expression...expression of the SPH activator paired with AAV sgRNA delivery make the SPH mouse an attractive tool to...Synapsin-1 (pan-neuronal) promoters (Figure 1b), and sgRNAs can be found here. Neuron-optimized viral vectors...lentivirus system pairs ubiquitous expression of sgRNA(s) and mCherry (vector 1) with neuron-specific expression...

  5. CRISPR Antimicrobials

    Type
    Blog Post
    ...Research Read Our CRISPR Guide Pages Find CRISPR gRNA Design Tools ...

  6. Lentivirus Plasmids

    Type
    Collection
    ...plasmids to express your CRISPR gRNA? Please see our CRISPR Empty gRNA plasmids table and search "lenti...

  7. Guide to Using Pooled Libraries

    Type
    Guide
    ...each plasmid contains a unique cDNA. In shRNA or gRNA libraries, each plasmid contains a unique gene targeting...library from the Moffat lab has over 175,000 different gRNA-containing plasmids). Amplifying and Using the Library... screen. The figure above is an outline of how a gRNA library is used. Below are the general steps to ...experimental and control cells Generate a list of gRNAs that disappear with addition of the selection mechanism...

  9. Plasmid Collections

    Type
    Collection
    ...libraries. Pooled Library Types cDNA shRNA CRISPR gRNAs Depositor Collections A modular set of plasmids ...

  11. Bacterial Expression Systems

    Type
    Collection
    ...see here . pgRNA-bacteria 44251 CRISPR Stanley Qi Expression of customizable guide RNA (gRNA) for bacterial...can be combined with expression of a gRNA from a plasmid like pgRNA-bacteria to knock down endogenous gene...Marraffini Bacterial expression of Cas9 nuclease gRNA. For a more comprehensive list of our bacterial ...

  13. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ... Cas13, upon finding complementarity between the gRNA and a given fluorescently labelled substrate, gets... E and the N2 gene of SARS-CoV-2. If the Cas12a-sgRNA complex binds to the target sequences, Cas12a activity...Fluorescently labelled dCas9 complexes containing sgRNA that have no mismatches bind to the immobilised ...

  16. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...75637 EIF2AK2 gRNA (BRDN0001146342) EIF2AK2 hU6 VWM disease David Root 75638 EIF2AK2 gRNA (BRDN0001148577...75639 EIF2AK2 gRNA (BRDN0001148668) EIF2AK2 hU6 VWM disease David Root 76195 ERBB4 gRNA (BRDN0001162372...David Root 76196 ERBB4 gRNA (BRDN0001149467) ERBB4 hU6 ALS David Root 76197 ERBB4 gRNA (BRDN0001146197) ERBB4...David Root 76198 ERBB4 gRNA (BRDN0001162324) ERBB4 hU6 ALS David Root 76809 PANK2 gRNA (BRDN0001149459) PANK2...76810 PANK2 gRNA (BRDN0001162474) PANK2 hU6 Hallervorden-Spatz disease David Root 76811 PANK2 gRNA (BRDN0001162362... PANK2 gRNA (BRDN0001144887) PANK2 hU6 Hallervorden-Spatz disease David Root 76838 PRKAR1B gRNA (BRDN0001146606...PRKAR1B gRNA (BRDN0001144765) PRKAR1B hU6 Dementia and/or parkinsonism David Root 76840 PRKAR1B gRNA (BRDN0001147089...

  18. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...position of the mismatch along the length of the gRNA given the stricter sequence requirement at the terminal...many software tools predict off-target effects of sgRNAs using computational methods. They identify possible...mismatches based on the sequences of the genome and sgRNA. This is a good starting point for most researchers...

  19. Microbiology Resources

    Type
    Collection
    ...CRISPR resources, such as depositor protocols and gRNA design software. Addgene yeast CRISPR resources ...

  20. Worm Expression Resources

    Type
    Collection
    ...resources include plasmids expressing Cas9, empty gRNA backbones, and constructs for endogenous tagging...
Showing: 181 - 200 of 241 results