...Location, Location, Location! Failure to Plan (for Storage) Is Planning to Fail Patience Is Bitter but Its... the transport issues. 2. Failure to plan (for storage) Is planning to fail Freeze/Thaw Cycles Of course...cycles can still reduce AAV titer. For long term storage, make > 25 μl aliquots in 0.5 ml tubes, snap freeze... liquid nitrogen or a dry ice/ethanol bath, and store at -80° C. Once thawed, the AAV aliquot should be... PubMed Central PMCID: PMC3098627. 6. Salegio, Ernesto A., et al. "Axonal transport of adeno-associated...

...documenting your plasmids, storing them, providing quality control and customer support, making plasmids...Yesterday we hit a huge milestone here at Addgene – 500,000 plasmids shipped! That's a 1/2 million plasmids...The trust of our depositors to allow us to help store and distribute their plasmids. The partnerships ...

...the CRISPR types and is thought to be the common ancestor of all other CRISPR systems. Type III systems ...(like me!) of CRISPR types — weird and poorly understood. There are three subtypes, A–C, and they are ...mechanism of type II is, unsurprisingly, well understood, and the first taught when introducing CRISPR...Covarrubias, P. C., Shmakov, S. A., Silva, C., Tolstoy, I., Johnson, D. B., Koonin, E. V., & Quatrini,...., Hunnewell, P., Alfonse, L. E., Carte, J. M., Keston-Smith, E., Sothiselvam, S., Garrity, A. J., Chong...

...equilibrium” where the cancer stops growing, and the immune system stops fighting. Such quiescent cancer...study cancer from every possible angle to leave no stone unturned, but cancer has been producing landslide...landslide after landslide, only adding more stones to the field. Fortunately, our bodies have been fighting ... downregulate the expression of the major histocompatibility complex (MHC) class I proteins on their surfaces...

...shared resources. As we step into 2025, these milestones remind us of the extraordinary impact that happens...shared progress. But numbers tell only part of the story. This year, we made significant strides in making... D., Cooper, J. A., Crosby, K., Harvey, K. J., Houston, D. W., Kumaran, R., Rego, M., Schofield, C., …...024-01095-8 Kahn, R. A., Virk, H., Laflamme, C., Houston, D. W., Polinski, N. K., Meijers, R., Levey, A....

...have helped Addgene grow and spread our mission.  Boston Museum of Science, where else? Addgenies and other...honored guests headed to the Museum of Science in Boston, where the entire Blue Wing was set aside for the...refresher on the Central Dogma of Biology, I would stop by later this year when it is displayed!).  The ...Joanne shared a moving thank you email from a requestor in Iraq (Addgene's first). After the speeches,...

...maintain high standards for both our depositors and requestors.  Requesting Plasmids available for Packaged ...quality control assays we run with all of our in-stock viral preps: ddPCR, purity testing, sterility testing...AAV2 to be 7x1012, the same titers found in our in-stock preps. If a request has technical considerations...considerations that may affect expected titers, a customer support specialist will reach out to you during the request...

...depositors, used by our requestors, and supported by our repository. You can enjoy these stories, and other 20th...rabies plasmids in our repository to international requestors.    I would be remiss not to mention that 2024...Throughout the year, we’ll be celebrating with stories of innovation and impact made possible by the sharing...

...applications in plants. This smaller, easily customizable vector functions in multiple species, including...for many applications, the plasmid can also be customized due to its modular structure. Two multiple cloning... regulatory elements to be inserted, further customizing gene expression. Sahoo et al. have tested the...Western blotting. Given its versatility and customizability, pSiM24 can be used not only for plant engineering...

...surely what is one of many awards that will be bestowed upon discovers of CRISPR/Cas9 genome engineering...panel discussion with Dr. Craig Mello and Dr. Paul Stoffels from J&J. The panel was moderated by a non-scientist...done with this technology to help patients. Dr. Stoffels and others in the audience discussed how J&J is...Technology? Learn More: Addgene's CRISPR/Cas9 Guide History of CRISPR Cas - A tale of survival and evolution...

...is especially exciting, including histone acetylation by p300, histone demethylation by LSD1, cytosine ... subpages, our updated CRISPR Guide and CRISPR History provide a great overview of the development of ...resources: CRISPR Plasmids and Resources and CRISPR History and Development for Genome Engineering  ...

...patient partners so that they can help tell their stories. I was paired with a six-year-old boy named AJ ...watch fellow Run4Rare team members complete the Boston Marathon, he wanted to run and play with my kids... as efficiently in humans. This is why we cannot stop searching for novel solutions to treat rare diseases...against FOP. Second, Addgene will be hosting the AJ’s Boston League team (Contributing many new heroes to the...

...Nina Dudnik, Founder of Seeding Labs, tells the story about working as a student in a rice lab in the ...supply house.  These large, cheap aliquots could be stored indefinitely at room temperature (thus also saving... powder in 50% nitric acid, but I never let that stop me. I only had to replenish my glass milk supplies... required less agar, less ethidium bromide, less stop buffer and miniscule amounts of plasmid sample.  ...

...indexing strategy that simultaneously tracks clonal history and cell identity at the single-cell level, thus... The CellTags revealed that cells with common ancestors tended to have similar reprogramming outcomes....results suggest that iEP reprogramming is not stochastic, but rather there’s a particular cell state that...track the dynamics of gene expression and clonal history associated with changes in cell identity. The Morris...

...entrepreneur in the health sciences?  Is this a different story altogether? The unfortunate answer is yes, largely...process. I am the cofounder and CEO of miLab, a Boston-based startup focused on empowering early-stage... is more important than the product itself -- investors would rather fund excellent people with mediocre...Harvard/MIT faculty, venture capital and angel investors, and business development heads at major companies...

...population (pink histogram) compared to the WT cells (green histogram). The unfilled histograms show cells ....3995 Virk, H. S., Biddle, M. S., Smallwood, D. T., Weston, C. A., Castells, E., Bowman, V. W., McCarthy, ...Z., Biddle, M. S., Wright, A. K. A., Sousa, J., Weston, C. A., Chachi, L., Roach, K. M., & Bradding, P...

...great deal more information that one might like to store about FPs than is currently represented at fpvis...a centralized, community-editable resource for storage and dissemination of information about FPs. FPbase.org...Users can “favorite” certain proteins, or create custom protein collections (such as a set of FPs that ...elaborate spectrum analysis tools, and a better way to store and represent within-study photobleaching comparisons...

...lab meetings with a focus on discussion and brainstorming Though, we might be forced to work from home...of each talk - time for free interaction and brainstorming. This could be achieved by asking each presenter...problem! You can still creatively collaborate and brainstorm! Image from Joseph Mucira.   Implement new...can be used as a break, but can also be used to brainstorm on data in smaller groups, where for instance...

...resources at Addgene. His background in history and specifically the history of RNAi left him a bit skeptical...Listen to the interview here! Doench majored in history during his undergraduate work at Hamilton College...just been discovered, the mechanism was barely understood.” Doench’s graduate work helped expand our understanding...

... by guest bloggers Joachim Goedhart and Marieke Mastop from the Section of Molecular Cytology and Van ... cells within the dish. This is caused by the stochastic nature of plasmid uptake by cells. To correct...our guest bloggers Joachim Goedhard and Marieke Mastop! Joachim Goedhart is an assistant professor at ...follow him on twitter: @joachimgoedhart.   Marieke Mastop is a PhD candidate at the Section of Molecular ...