Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
Type
Blog Post
...in our hands, is both cost-effective and highly active. After csRNP complex formation, a single-stranded...efficiently and serves as a good control for both the activity of CRISPR reagents and ICC antibody specificity...of the crRNA (aim for 40-60% GC content)
crRNA activity
Local chromatin structure (although we could successfully...turnaround time 5-7 days); because the tracrRNA is the same in all csRNP experiments, it can be ordered in bulk...