...SpCas9 is fused to the puromycin resistance gene through a 2A peptide. The puromycin resistance gene enables...causes the ribosome to translate Cas9-2A and the Puromycin resistance gene as separate peptides. PX459 This...predecessor, which had a point mutation in the puromycin resistance gene that made it less effective in...

...marker such as puromycin, you can (also) select for the positive colonies by puromycin selection. You ...transfected cells (for example, plasmid with a puromycin resistance cassette, such as PX459). After you...

...Nathans, Daniel. "Puromycin inhibition of protein synthesis: incorporation of puromycin into peptide chains...Yarmolinsky, Michael B., and L. Gabriel. "Inhibition by puromycin of amino acid incorporation into protein." Proceedings...

...but was found to have a point mutation in the puromycin cassette that makes it less efficient in some ...with customizable sgRNA from the U6 promoter and puromycin resistance from the EF-1a promoter. It’s a 3rd...PX459) V2.0. It contains a point mutation in the puromycin cassette that makes it less efficient in some ...

...stably integrate a reporter gene that contains a puromycin resistance gene followed by the target site (the...genome edit:  A donor plasmid that contains a puromycin resistance gene linked to an in-frame H2B-GFP....

...- and locus-specific gRNAs Step 4: Select for puromycin resistant cells Step 5: PCR amplify and sequence...cassette into the FBL locus. Upon sequencing of puromycin resistant clones, they found that 80% and 50% ...

...kinase promoter drives expression of puromycin. Puro R Puromycin resistance gene for selection of pLKO...sulfate, and puromycin are located in the “Appendix”. F.2. Determining the Optimal Puromycin Concentration...differently to puromycin selection. Addgene strongly recommends that you determine the optimal puromycin concentration...to fresh puromycin-containing media every other day. f. The minimum concentration of puromycin that results...with finer increments of puromycin to determine a more precise optimal puromycin concentration.) F.3. Protocol...expression of the puromycin resistance gene. f. To select for infected cells, add puromycin to the media at...positive control for the puromycin selection. Days 4+: g. Change to fresh puromycin-containing media as needed...

...different selection markers (blasticidin, neomycin, puromycin, hygromycin) and fluorescent protein reporters...

...Using translation inhibitors cycloheximide and puromycin, Halstead et al. verified that translation is ...

...CD90.1 selection marker, Thy1.1, in addition to puromycin resistance.  The MYC-CRISPR library targets E-...

... genes along with 1000 non-targeting controls Puromycin Doench , Root 73179 Human gRNA pooled library ... genes along with 1000 non-targeting controls Puromycin Doench , Root Human activation pooled libraries...56,762 unique sgRNAs targeting 18,885 human genes Puromycin Doench , Root Human inactivation pooled libraries...human genes along with 500 non-targeting controls Puromycin Doench , Root 92386 Human CRISPR inhibition library...human genes along with 500 non-targeting controls Puromycin Doench , Root Mouse knockout pooled libraries ... genes along with 1000 non-targeting controls Puromycin Doench , Root Cas9 Lentivirus Cas9 viruses can...

...80S; inhibits chain elongation. 50-500 ug/mL Puromycin pac HeLa, 293HEK, Jurkat T cells Inhibits protein...

...YET Cre is coexpressed with tdTomato and Pac (puromycin N-acethyl-transferase) pCDH-CB-copGFP-T2A-iCre...pCDH-CB-FLPe-P2A-copGFP-T2A-Puro 72258 Expresses FLPe, copGFP and the puromycin resistance from the CB promoter pCDH-CB-FLPe-P2A-tdTomato...-copGFP-T2A-Puro 72263 Express copGFP and the puromycin resistance gene from the EF1 promoter pCDH-CMV-mCherry-T2A-Puro...pCDH-CMV-mCherry-T2A-Puro 72264 Express mCherry and puromycin resistance gene from the CMV promoter pCDH-CMV...

...Tyler Jacks. It carries CRE recombinase and a puromycin resistance cassette. No time to produce virus...

...lentiviral backbones expressing blasticidin or puromycin resistance genes.  It’s that simple! Good luck...

...cells where recombination has occurred. Neomycin, puromycin, and hygromycin resistance genes are commonly ...

...YFP, Strep, TEV-V5) with either blasticidin or puromycin selection. Researchers at the Allen Institute ...

... 11586 pSico PGK puro Cre addition causes the puromycin gene to be recombined out of the construct, activating...12084 pSicoR PGK Puro Cre addition causes both puromycin and shRNA to be recombined out of the construct...Derivative of pMKO.1 puro with GFP instead of puromycin resistance gene William Hahn Additional Resources...

... vectors for CROPseq-multi using selection by Puromycin, Zeocin®, or nuclear expression of BFP2.   ...

... . The following day, cells were treated with puromycin. Colonies were allowed to grow in the presence...