Skip to main content
Addgene
Showing: 1 - 20 of 24 results

  1. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ...translocation reporter with a mRuby2 fluorescent reporter (ERK KTR-mRuby2). The ERK KTR is a target for...For testing kinase inhibitors, psMEK1 and ERK KTR-mRuby2 expressing cells were incubated with an inhibitor...activate psMEK1, and the localization of ERK KTR-mRuby2 determined. If the inhibitor targeted MEK1 or its...the cytoplasm. This all-optical psMEK1 + ERK KTR-mRuby2 system has several advantages over existing methods...

  2. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ... the C-terminus of LSSmOrange mRuby2 Red Mammalian Expresses mRuby2 (a RFP variant) commonly used with...Expresses Clover (a GFP variant) commonly used with mRuby2 pLSSmOrange-N1 Orange Mammalian Express a gene...

  3. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ...Serotype Name 124603 AAV9 pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40 124650 AAV9 pAAV-CamKIIa-C1V1...pAAV-Ef1a-fDIO mCherry 99130 AAVrg pAAV-mDlx-NLS-mRuby2 112677 AAV1, AAV2 pOTTC1032 - pAAV EF1a Nuc-flox...

  4. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    ...pAAV-FLEX-tdTomato pAAV-GFAP104-mCherry pAAV-mDlx-NLS-mRuby2 CAG-NLS-GFP pAAV-hSyn-mCherry Read on to learn...pAAV-FLEX-tdTomato, pAAV-GFAP104-mCherry, pAAV-mDlx-NLS-mRuby2, CAG-NLS-GFP,and pAAV-hSyn-mCherry at Addgene. ...

  5. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    ...wavelength red fluorescent proteins like TagRFP or mRuby2 than longer wavelength proteins like mCherry. For...mTagBFP2, EGFP or one of the improved GFP variants, mRuby2 or TagRFP-T, and an infrared fluorescent protein...

  7. New Viral Vectors - March 2024

    Type
    Blog Post
    ...AAV2 Controls Fishell New serotype pAAV-mDlx-NLS-mRuby2  AAV PHP.eB Controls Gradinaru New serotype pAAV-CaMKIIa-mCherry...

  11. Hot Plasmids - February 2022

    Type
    Blog Post
    ...set of new anti-FLAG frankebodies includes mEGFP, mRuby2, iRFP713, SNAP-tag, and HaloTag fusions, while ...

  12. Biosensor AAV Preps

    Type
    Collection
    ... GCaMP6f 50943 pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA Syn GCaMP6f mRuby2 Constitutive 1 Bonhoeffer ...Khakh 68719 pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA CAG GCaMP6f mRuby2 Cre dependent 1 Bonhoeffer... GCaMP6s 50942 pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA Syn GCaMP6s mRuby2 Constitutive 1 Bonhoeffer ... Ting 68717 pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA CAG GCaMP6s mRuby2 Cre dependent 1 Bonhoeffer...Rose 68720 pAAV-hSyn1-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA Syn GCaMP6s mRuby2 Cre dependent 1 Bonhoeffer...164510 pAAV.hSynap.(cyto)iGlucoSnFR-mRuby2 Syn (cyto)iGlucoSnFr mRuby2 Constitutive 9 Looger Norepinephrine...

  13. Brain Initiative Collection

    Type
    Collection
    ...99130-AAV1 pAAV-mDlx-NLS-mRuby2 An AAV genome that expresses nuclear localized mRuby2 from the mouse Dlx5/...99130-AAV2 pAAV-mDlx-NLS-mRuby2 An AAV genome that expresses nuclear localized mRuby2 from the mouse Dlx5/...99130-AAV8 pAAV-mDlx-NLS-mRuby2 An AAV genome that expresses nuclear localized mRuby2 from the mouse Dlx5/...99130-AAV9 pAAV-mDlx-NLS-mRuby2 An AAV genome that expresses nuclear localized mRuby2 from the mouse Dlx5/...-AAVrg pAAV-mDlx-NLS-mRuby2 An AAV genome that expresses nuclear localized mRuby2 from the mouse Dlx5/...-PHPeB pAAV-mDlx-NLS-mRuby2 An AAV genome that expresses nuclear localized mRuby2 from the mouse Dlx5/...pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and targeted...

  14. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...to the C-terminus of LSSmOrange mRuby2 Red Mammalian Expresses mRuby2 (a RFP variant) commonly used with...Expresses Clover (a GFP variant) commonly used with mRuby2 pLSSmOrange-N1 Orange Mammalian Express a gene ...

  15. Optogenetics AAV Preps

    Type
    Collection
    ...105448 pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST Syn ChrimsonR (soma-targeted) mRuby2 Cre dependent 9 Adesnik 124603...pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40 EF1a ChrimsonR (soma-targeted) mRuby2 Cre dependent 9 Harvey...

  16. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...Oliver Griesbeck AV-1-50942 50942-AAV1 pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA Tobias Bonhoeffer, Mark...Huebener, Tobias Rose AV-1-50943 50943-AAV1 pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA Tobias Bonhoeffer, Mark...Tobias Rose AV-1-68717 68717-AAV1 pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA Tobias Bonhoeffer, Mark...Tobias Rose AV-1-68719 68719-AAV1 pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA Tobias Bonhoeffer, Mark...Tobias Rose AV-1-68720 68720-AAV1 pAAV-hSyn1-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA Tobias Bonhoeffer, Mark...Vincent Pieribone AV-1-51473 51473-AAV1 pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6m-WPRE-pA Tobias Bonhoeffer, Mark...

  17. Control AAV Preps

    Type
    Collection
    ...rg*, PHP.eB Fishell 99130 pAAV-mDlx-NLS-mRuby2 Dlx NLS-mRuby2 Constitutive 1, 2, 8, 9, rg*, PHP.eB Gradinaru...

  18. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Expression pFA6a-link-yomRuby2-SpHis5 - Yeast Expression mRuby2-N1 - Mammalian Expression mRuby2-C1 - Mammalian...Bacterial Expression mRuby2 559 600 43 5.3 150 min Prone to dimerization pcDNA3-mRuby2 - Mammalian Expression...

  19. Caltech Systemic Capsids

    Type
    Collection
    ...Dlx GFP Control Fishell 99130 pAAV-mDlx-NLS-mRuby2 Dlx mRuby2 Control Gradinaru 104055 pAAV-CAG-eYFP CAG...

  20. Retrograde AAV viral preps

    Type
    Collection
    ... Control Deisseroth 99130 pAAV-mDlx-NLS-mRuby2 Dlx NLS-mRuby2 Control Gradinaru 83900 pAAV-mDlx-GFP-Fishell...
Showing: 1 - 20 of 24 results