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  2. Michael Davidson Fluorescent Protein Collection

    Type
    Collection
    ...Description Industry Attachments mEmeralds Plasmid Description Industry Attachments Reds Plasmid Description...Description Industry Attachments Blues Plasmid Description Industry Attachments Cyans Plasmid Description ...Description Industry Attachments Oranges Plasmid Description Industry Attachments Greens Plasmid Description Industry...Industry Attachments Yellows Plasmid Description Industry Attachments Photoactivatables Plasmid Description...Description Industry Attachments Do you have suggestions for other plasmids that should be added to this list...

  3. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ... you'll find in this post: MagIC-Cryo-EM for enrichment and structure determination LEA proteins protect...marking astrocytes MagIC-Cryo-EM for sample enrichment and structure determination By Mike Lacy Conventional...and Concentration (MagIC)-cryo-EM that combines enrichment and imaging of proteins captured on magnetic ...proteins ending with a GFP nanobody (Figure 1). This enrichment streamlines the purification and minimizes sample...    Figure 1: MagIC-cryo-EM for sample enrichment and structure determination. A) Schematic of ...

  4. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...and easier to use with other strains of E. coli, Schmidl et al. condensed the system to two streamlined ...gene expression at very high or very low levels. Schmidl et al. chose to optimize these systems to make ...enable the assembly of larger systems. The work of Schmidl et al. also represents a large step forward in ...light-switchable Escherichia coli two-component systems. Schmidl SR, Sheth RU, Wu A, Tabor JJ. ACS Synth Biol. 2014...

  5. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    ...display to increase the enrichment of target-specific sybodies. Final enrichment via enzyme-linked immunosorbent...Ribosome display The Seeger lab starts with a “pre-enrichment” selection step using ribosome display because...sybody candidate. These tags are used in a final enrichment step for identifying sybodies that bind strongly...

  6. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    ... fluorescence. Transient reporter for editing enrichment (TREE) The Wang and Brafman lab developed a transient... transient reporter for editing enrichment (TREE) to purify single base edited cells without the need ...A fluorescent reporter for quantification and enrichment of DNA editing by APOBEC–Cas9 or cleavage by ...Brafman DA (2019) A transient reporter for editing enrichment (TREE) in human cells. Nucleic Acids Research...

  7. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...doi.org/10.1021/acssynbio.0c00581   New tool for enrichment of ABE-targeted cells By Ellie Adams Single base...mediated adenosine transient reporter for editing enrichment”, or XMAS-TREE, to identify base edited cell ...the past.   Figure 1: Identification and enrichment use of XMAS-TREE. a) Cells are transfected with...

  8. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...generated a strong streptavidin signal (~250-fold enrichment) when compared to a scrambled RNA motif control...labeling with BASU still generated a >30-fold enrichment compared to E. coli’s BirA*. Faster labeling ...-associated sequences had lower IREB2 binding enrichment compared to wild-type sequences and that loss...

  10. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...pLNCX2-mEGFP-CHMP2B CHMP2B GFP CMV ALS Sanford Simon 115331 pLNCX2-mCherry-CHMP2B CHMP2B mCherry CMV ALS...Dementia Gerold Schmitt-Ulms 61857 MLM3636-Prnp-CDS-Pos PRNP hU6 Dementia Gerold Schmitt-Ulms 61946 4QQ6...LTR VWM disease Seiichi Oyadomari 101848 pTO_CHMP2B_LAP CHMP2B LAP CMV ALS Daniel Gerlich 101874 pEYFP-...ALS Sanford Simon 115333 pLNCX2-mCherry-CHMP2B-siRNAres CHMP2B mCherry CMV ALS Sanford Simon 115523 MSCV-human...Spinocerebellar ataxia 6 Michael Ward 178153 CHMP2B_Halo_C_allele CHMP2B Halo ALS Michael Ward 178154 COQ2_Halo_N_allele...2N4R-EGFP MAPT GFP TREtight Parkinson's, FTD Gerold Schmitt-Ulms 132393 AAVS1 CAG rtTA3 TauP301L 2N4R-EGFP ...2N4R-EGFP MAPT GFP TREtight Parkinson's, FTD Gerold Schmitt-Ulms 133320 pHBS834 H14-SUMO-TDP43 WT-TEV-mCherry ...

  11. A Scientist's Guide to Ebola

    Type
    Blog Post
    ...virion surface – making it critical for viral attachment and fusion. Once inside a host cell, new evidence... of neutralizing antibodies and inhibitors of attachment and fusion. Recommended Reading For those looking...

  15. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ...population is key. Loss of representation due to enrichment or depletion of specific gRNAs can lead to skewed...several cell types can strengthen your results. Enrichment or depletion of multiple gRNAs targeting the ...

  16. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...., Allam, A., Kissling, L., Marquart, K. F., Schmidheini, L., Solari, C., Balázs, Z., Krauthammer, M.,... A., Tálas, A., Kissling, L., Benvenuto, E., Schmidheini, L., Schep, R., Damodharan, T., Balázs, Z., Janjuha...

  18. An Introduction to Adenovirus

    Type
    Blog Post
    ...biology of adenoviruses (AdVs). AdVs are ubiquitous (Schmitz et al., 1983), yet rarely cause severe disease ...doi.org/10.3181/00379727-84-20714. PMID: 13134217. Schmitz, H., Wigand, R., & Heinrich, W. (1983). Worldwide...

  19. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...is used to separate the beads from the media (Brechmann et al. al., 2021) while the media is aspirated....2019.12.017. Epub 2019 Dec 28. PMID: 31891733. Brechmann NA, Schwarz H, Eriksson PO, Eriksson K, Shokri...

  20. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    ...phosphopantetheinyltransferase) – These enzymes catalyze the covalent attachment of a fluorophore ligand to a peptide tag (Figure... the speed and selectivity of the fluorophore attachment, the brightness of the resulting conjugate, and...
Showing: 1 - 20 of 110 results