...define plasmid host range, identify a few of the features that confer broad host range in plasmids in ... features to create broad host range recombinant vectors.  A plasmid’s host range is determined by the... a narrow host range, while others have a broad host range and can replicate and stably maintain the genes...been identified as broad host range plasmids. The properties that influence host range are not fully understood...examples of a narrow host range plasmid with only one ori and a broad host range plasmid with multiple... proteins independent of the host. Host range depends on whether the host has the components to recognize...DnaA-box and iteron structure, host-independent   Building broad host range recombinant vectors Replicons...

...Additionally, these viruses are able to target a broad range of dividing and non-dividing cell types with ...retroviruses , adenoviruses do not integrate into the host genome. Disadvantages of adenoviruses include transient...

...with the RSF1010 origin, an IncQ origin with broad host-range (Meyer, 2009). The vector retains the oriT...designed to study the bee microbiome, these broad-host-range plasmid could also be used to study other .... Find the toolkit for modular assembly of broad host range plasmids Counting cell division in the gut...bacteria with a toolkit for modular assembly of broad-host-range plasmids." ACS synthetic biology 7.5 (2018...Replication and conjugative mobilization of broad host-range IncQ plasmids." Plasmid 62.2 (2009): 57-70...replicative origins that can be used for broad or narrow range of microbial species. These also contain...recipients by conjugation. The labs screened for the host-range of the plasmids and suitability of various antibiotic...

...Operon Bacterial expression of luciferase from broad host range plasmid Attila Karsi 105621 Lenti-luciferase-P2A-Neo...dynamic range, and resistance to photobleaching has made luciferase a common choice in assays ranging from...bioluminescence found in a diverse number of organisms, ranging from bacteria to aquatic animals to insects. Luciferase...of expression. Scientists have developed a wide range of luciferase-based plasmid systems for many different...brighter signal and emission in the near-infrared range, enabling enhanced bioluminescence imaging in vivo...toolkit to assess the proteostasis status in a wide range of experimental systems. LumiLuc and LumiScarlet...

...pTac GFP, Luciferase (LuxCDABE) Attila Karsi Broad host range vectors for expressing luciferase and fluorescent...dissect an individual protein’s function under a wide range of highly controlled conditions in vitro , or, if... suitable for replication in many gram-negative hosts. pCri System Various Plasmids T7/lacO and lacO variants...Various Plasmids Blue/UV , Cyan , Green , Yellow , Orange , Red , Far-Red , Near-Infrared , Large Stokes ...

...infect cells and organisms (which we refer to as hosts) via viral particles, the functional infection unit...engineered to be cell type specific. Once inside the host cell, the virus unleashes its cargo – its own genome...   Fig. 1: Viral particle infection into a host cell. Image courtesy of National Human Genome Research...spread. Instead, viruses replicate by hijacking the host’s system to produce more virus and continue the infection...cargo will either permanently integrate into the host genome or temporarily express whatever it encodes...transduction efficiency and can effectively infect a wide range of cell types that are typically ‘difficult to transfect...viruses that stably integrate their genome into the host genome, setting up permanent residence. They primarily...

... another virus to either restrict or broaden the host cell range (tropism). Pseudotyping is only done ...corresponding receptor in the host cell. Some of these envelope-receptor pairings are broad, allowing delivery into... viruses to infect neurons. Besides altering the host tropism of your virus, there are many other reasons...plasmid. Image from Crawford et al., 2020.   Broad tropism: VSV-G pseudotyping A commonly used envelope...transduce stem cells and neurons. However, VSV-G’s broad tropism can be undesired for in vivo studies since...envelope proteins for pseudotyping that could be broad or specific to suit your needs. Check out some of...

...integration of the transfer plasmid sequences into the host genome. Typically, it is the sequences between and... including the LTRs that are integrated into the host genome upon viral transduction. Most transfer plasmids...the VSV-G envelope provides the widest tropism or range of cells a virus can infect. Note: While both lentiviruses...dividing and non-dividing cells because once in the host cell cytoplasm, the viral components are small enough...-G Vesicular stomatitis virus G glycoprotein; Broad tropism envelope protein; Pseudotyped to alter infectivity...

...which is a very diverse family of viruses with broad host range and tissue tropism (Borkenhagen et al., 2019...., 2023).  This anecdote goes toward saying that host-virus interactions are an important part of the ...are available on the commercial market). Although host-virus interactions likely seem important for any... cycle of AdVs is dependent on modulation of the host cell’s cell cycle; inhibition of the innate immune...

...the LDL receptor to infect cells and have a broad host range. However, viruses pseudotyped with other envelope...the media becomes cloudy. Mycoplasma compete with host cells for nutrients and can alter expression of ... To overcome this issue, try transducing with a range of dilutions of your lentiviral prep. Hopefully,...

...Lentiviral particles can efficiently infect a broad range of cell types, including both dividing and non-dividing...the Whitehead Institute for Biomedical Research hosts an siRNA Selection Program that can be accessed ...created. Additional design rules can be found at the Broad Institute's TRC shRNA Design Process . To minimize...but the DNA is unlikely to be integrated into the host genome. For stable loss-of-function experiments,...particles per cell. Addgene recommends that you test a range of MOIs to determine the optimal MOI for infection.../mammalianrnai The RNAi Consortium (TRC): www.broad.mit.edu/genome_bio/trc/rnai.html Background on RNAi...

...microbiome. They created a modular system using a broad host range plasmid that can be maintained in a number...Bacteria with a Toolkit for Modular Assembly of Broad-Host-Range Plasmids. ACS Synth Biol 7:1279–1290 . https...organisms or organism/gene interactions with the host. This scenario allows for several potential CRISPR...Vridhambal GS, Rivera AJ, Montgomery SA, Fortier L-C, Barrangou R, Theriot CM, Ousterout DG (2020) In Vivo Targeting...

... an origin of replication (which controls the host range and copy number of the plasmid) and typically...enable molecular biologists to broadly utilize plasmids across a wide range of applications. Some common...pieces of DNA that replicate independently from the host's chromosomal DNA. They are mainly found in bacteria...plasmids provide one or more functional benefits to the host such as resistance to antibiotics, degradative functions...

...These are all normal outcomes. If you have a broad range of mentors you will always have people you can...programs. The Healthcare Businesswomen’s Association hosts an excellent group mentoring program in many of ...and be able to discuss more confidential topics Broaden your network Take advantage of formal programs...

...infecting a host cell. This viral DNA (also called proviral DNA) is then inserted into the host's genome and...immunogenicity, meaning they induce an immune response in the host. So why use a gamma-retrovirus in the lab? Well...experimental needs, and can transduce (infect) a wide range of cell types with a high efficiency. However, gamma-retrovirus... gamma-retrovirus including genome architecture, host integration, and its association with disease. The...essentially remaining an extrachromosomal part of the host genome that can replicate and divide along with ... to induce a lower inflammatory response within hosts. Relative to the other viruses discussed in this...All viruses discussed in this blog are generally broadly infective, with one exception. Blood cells are ...

...experiments? In this post, we will discuss some broad classes of optogenetic tools, and what factors to...that can change both the membrane potential and a host of other cellular functions. Photoswitches are engineered...activity upon exposure to light within a specific range of wavelengths. Excitatory channels allow positively...engineered receptors. OptoXRs are great for studying the broad effects of activating a particular receptor or when...optimal wavelength to use but can be activated by a range of wavelengths. While this may give you a little...

...AAVs are non-pathogenic, do not integrate into the host genome, and can be specifically targeted to a number...human therapies and in vivo laboratory experiments ranging from transient gene expression in a particular ...application of AAV is delivery of genetic cargo to a host cell. In order to make room for a gene of interest... to expression AAVs have to infect cells, hijack host cell machinery, and synthesize a second strand of...2013). Remember that AAVs do not integrate into the host genome and therefore the transduced gene is episomal...does not divide and replicate in the same way the host DNA does. Because of this, fast dividing tissues...expression, since you don’t have to wait for the host to replicate the DNA before expression. Now that...

...spectra of the fluorescent molecules can be quite broad, detectors will usually only pick up a small portion...the markers in the panel. This complexity index ranges from 0-1, with a value of “0” indicating those ...Lila Witt, a Ph.D. student at Mayo Clinic studying host-microbe interactions in early life.    Resources...

... variants have been created, thereby enabling a broad spectrum of donor fluorescence wavelengths for FRET...substantial: Plasmid tRNA Synthetase Expression Host pMAH-POLY Polyspecific aminoacyl-tRNA Synthetase... a problem, as long as they are beyond the FRET range of the donor (i.e. >100 Å). Researchers have developed... above can be paired together to measure a wide range of molecular distances (see Table). However, proper...experiments are needed to establish the dynamic range of your assay. These experiments include producing...you can expect, thereby establishing the dynamic range of your FRET assay (Figure 2). These labeling methods...FRET experiments. Donor Acceptor Tag R0 (Å)a Range (Å)b Applications CFP   Lumio Green Tc: CCPGCC...

...the virus.  VSV-G is very common due to its broad host range, and increased particle stability; however...