...for in our reported titers. Lentiviral vectors are titered using a lentiviral droplet digital PCR (ddPCR...serial dilutions of a lentiviral vector, incubated for 72 hours, and genomic DNA is extracted. Primers...protocols. Browse our AAV vector collection. Lentivirus Production Lentiviral preparations ("-LV” catalog... with viral vector manufacturing facilities, such as the University of Pennsylvania Vector Core. Transfections...Pennsylvania Vector Core. In general, titering is performed by the facility that produced the viral vector lot....Empty vector particles can be identified after negative staining and appear darker than full vector particles...Electron micrograph of AAV vector preparation shows that the vast majority of the vectors consist of full particles...

... for using pooled lentiviral libraries. A) Amplification, validation, and lentiviral generation of a pooled...Usage of a pooled library lentiviral prep for experiments. Pooled library lentiviral preps are generally aliquoted...the pooled library DNA, allow the bacteria to grow and replicate, then purify the DNA.     Figure...Producing Your Lentiviral Library Once you've amplified–and verified!–your pooled library DNA, the next step...Pooled Lentiviral CRISPR Libraries- A nice overview of what to consider when using pooled lentiviral CRISPR...production of lentivirus. Purification of this lentivirus results in the pooled library lentiviral prep. B)...Most of the pooled libraries at Addgene are lentiviral pooled libraries, though other types exist.  When ...

...blog Viral Vectors 101: Viral Vector Elements Viral Vectors 101: Types of Viruses Viral Vectors 101: Viral...that have been developed into lentiviral vectors. Lentiviruses are sometimes referred to as “complex ...from a reverse-transcribed piece of DNA called a “provirus.” This DNA is synthesized inside of what is called...would not be great for a gene therapy vector! For viral vectors, only the protein for reverse transcriptase...components of the vector particle, some of the RT protein gets packaged inside of the vector particle, along...double stranded DNA is integrated into the host chromosome, and this is where viral vectors and natural viruses...Resources on addgene.org Addgene's Lentiviral Guide Most popular lentiviral plasmids γ-Retrovirus Guide References...

...plasmids are formed. Viral vectors are prone to recombination In lentiviral vectors, recombination often occurs...noticed that in Stbl3 bacterial cultures with lentiviral vectors, the flat white colonies contained the full...Remarkable stability of an instability-prone lentiviral vector plasmid in Escherichia coli Stbl3. 3 Biotech...Improved recombinational stability of lentiviral expression vectors using reduced-genomeEscherichia coli...problems that I help troubleshoot involves viral vectors and plasmids with repeating sequences. Repeating...sequences within a plasmid can undergo intramolecular DNA recombination. This naturally occurring process is.... Over time, bacteria containing the recombined vector backbone will also outgrow bacteria that contain...

...of viral vectors here! Need Virus? Check out Addgene's Viral Service! Types of viral vectors Of the many...cargo from RNA to DNA and permanently insert the cargo into the host genome. Lentiviral-specific components...of use. Our lentiviral guide outlines these components.     Fig. 1 - Viral vector components of...types, but most vectors are based on adenovirus 5 (Ad5).     Fig. 3 - Viral vector components of...of adenovirus Building viral vectors Addgene has many viral vectors and helper plasmids for all the...gamma-retrovirus, lentivirus, adenovirus, and adeno-associated virus (AAV). The components of these vectors are also...’ll breakdown the vector components first by virus and then by function. Lentivirus/gamma-retrovirus Lentiviruses...

...generation of lentiviral vectors consisted of three plasmids encoding 1) the lentiviral vector genome which...Research Lentiviral Vector Uses and Overview Resources on Addgene.org Find Lentiviral Vectors for Your...property makes the lentivirus an attractive choice for a gene transfer vector. Lentiviral vectors Although retroviruses...plan your experiment using the lentiviral vector system. Viral vectors have been increasingly popular ...primary cells. Amongst the different vectors used, lentiviral vector constructs have proven very useful ...transfer vectors The most widely used inducible lentiviral vector system is the tetracycline (Tet)-regulated ...If you are interested in using lentiviral vectors to introduce your favourite gene into your favourite...

...systems, see our Lentiviral Plasmid FAQs. Lentiviral vectors in the lab Lentiviral vectors are incredibly...screening.   Lentiviral vectors in the clinic Since lentiviral vectors can deliver a large amount of DNA (~8 kb...current (and future) applications of lentiviral vectors! Lentiviral vector history and components In the early... are also working to make lentiviral vectors safer. Since lentiviral vectors integrate into the genome...Lentiviral vectors are one of the most popular and useful viral vectors in the lab. Advantages of lentivirus... dividing and non-dividing cells. Lentiviral vectors are the vector of choice for many CRISPR applications...adeno-associated viral vectors can also target non-dividing cells, lentiviral vectors can carry much more...

...viral vector backbones; once validated these universal primers can be used to titer any lentiviral prep... and titration of pseudotyped HIV-1-based lentiviral vectors."Nature protocols 4.4 (2009): 495-505. PubMed... Geraerts, Martine, et al. "Comparison of lentiviral vector titration methods."BMC biotechnology 6.1 (... significance of controlled conditions in lentiviral vector titration and in the use of multiplicity of...Addgene Read Our Lentiviral Guide Check out All of Our Viral Vector Resources ...packaging cell line, prepped your DNA, transfected and harvested your lentivirus. Now it’s time to move ahead...considerations for titering lentivirus Volume and transduction time will influence lentiviral titers as will the...

... Cre-lox lentiviral vectors: This approach is the same as using a standard lentiviral vector, but transient...safe for clinical applications.     Lentiviral: Lentiviral vectors infect dividing and nondividing cells...reprogramming vectors available from Addgene. Another drawback is that lentiviral vectors insert in different...of minicircle RNA Delivery DNA-Free High High Transgene-free and vector-free Multiple transfections ... required Protein Delivery DNA-Free Low High Transgene-free and vector-free. Slow and inefficient Difficult...so either a single polycistronic vector or multiple single-gene vectors can be used to package reprogramming...a “cut and paste” mechanism between a vector and chromosomal DNA. PB has a cloning capacity of ~9-14 kb...

...  Find the scrambled shRNA vector. pLKO.1 - TRC cloning vector - This vector from the David Root lab is...It can be used with 2nd or 3rd generation lentiviral vectors and an envelope expressing plasmid, such ... lentiCRISPR v2 - This 3rd generation lentiviral transfer vector also from the Zhang lab is used to deliver... David Sabatini’s lab is a 3rd generation lentiviral vector that contains a scrambled shRNA. This scrambled...our CRISPR posts Read our viral vector blog posts Read our lentivirus guide Resources on the Addgene ...opening our UK office in 2014, to starting our viral vector service in 2016...we’re excited to help scientists...the last 15 years - the majority of these being lentiviral or CRISPR tools. How many times have these plasmids...

... of Gateway cloning vectors Vector type  Vector features Purpose Donor vector attP sites for recombination...move DNA sequences into multiple vector systems. With the appropriate entry and destination vectors, one...multiple DNA fragments into many vectors at once in the same tube. You can clone up to 4 DNA fragments...frame When you move a DNA fragment from one Gateway vector to another, the inserted DNA fragment stays in ...Universality All types of DNA fragments may be cloned: PCR fragments, cDNA or Genomic DNA and is available for...clone with the DNA of interest flanked by attB sites is generated. As in the BP reaction, a DNA fragment containing...ccdB gene is present in the donor vectors and the destination vectors prior to recombination, and it is...

...designed to have increased lentiviral titer as compared to previous lentiviral vectors. It can be used both ...its name suggests, lentiCRISPR v2 is a lentiviral transfer vector used to deliver both Cas9 and your gRNA...single empty vector and in pooled libraries containing thousands of pre-cloned gRNAs. DNA aliquots of ... the Addgene Website Visit our Lentiviral Guide Pages Find Lentiviral Plasmids Find CRISPR Pooled Libraries...Neville E., Ophir Shalem, and Feng Zhang. "Improved vectors and genome-wide libraries for CRISPR screening....backbone for their many gRNAs. The high titer lentivirus production afforded by lentiCRISPR v2 is key ...

... lysed bacteria and can include plasmid DNA or fragmented DNA released into the environment. Various factors...foreign DNA or RNA is introduced into bacterial or eukaryotic cells via a virus or viral vector. One example...bacterial DNA can be accidentally packaged into the newly created phages. Transfer of this DNA to another...Transferred DNA once inside the infected bacterium can either exist as transient extrachromosomal DNA, like ...this scientists commonly use phagemids, a DNA cloning vector that contains both bacteriophage and plasmid...foreign DNA into eukaryotic cells, like mammalian cell lines. This can be done with lentiviral and Adeno... information on viral vectors, including transduction download our Viral Vectors 101 eBook. Bacterial ...

... is a dual-vector system, meaning it is typically used in conjunction with a second vector that delivers... gRNA lentiviruses). Separating Cas9 and the gRNA into two constructs (i.e., using a dual-vector system...lentiCas9-Blast Most of Addgene’s lentiviral inventory is based on CRISPR tools, though we do provide a... Cas9 nuclease, which introduces double stranded DNA breaks, can be expressed from lentiCas9-Blast. Purpose...LentiCas9-blast LentiCas9-blast is a Cas9 expression vector developed by Feng Zhang’s lab at the Broad Institute...such as lentiGuide-Puro. The alternative single-vector systems typically express Cas9 and a gRNA from ...system as opposed to a single-vector system) has several benefits. First, the low viral titers typically ...

...Unmethylated DNA not degraded, cell still can methylate DNA   hsdS(rk-,mk-) Unmethylated DNA not degraded...cytosine DNA Allows for uptake of foreign (methylated) DNA recA, recA1, recA13 Mutation in a DNA-dependent...cloning and storage of unstable DNA and lentiviral/retroviral vectors. Used by Addgene for pooled library... routine cloning, cloning unstable DNA, preparing unmethylated DNA, and more. Many mutations that make... Consequence dam DNA adenine methylase mutation (GATC) Preparing unmethylated DNA, important when trying...ClaI or XbaI) dcm DNA cytosine methylase mutation (CCWGG) Preparing unmethylated DNA, important when trying...cleaves methylated cytosine DNA Allows for uptake of foreign (methylated) DNA mrr, Δ(mcrC-mrr) Inactivation...

... While lentiviral vectors are popular gene delivery tools, producing lentivirus, can pose certain challenges...Blog Your lentiviral FAQs answered Tips for titering your lentivirus Visit the viral vector featured topic...Resources on Addgene.org Lentiviral Guide Find lentivirus plasmids Browse all viral vectors ...different generations of lentiviral packaging systems please see Addgene’s Lentiviral Guide. While the third...more tips on lentiviral titering please see our blog post. Between choosing the best lentiviral system, producing...Systematic determination of the packaging limit of lentiviral vectors. Hum Gene Ther. 2001 Oct 10;12(15):1893-905...Talking 'bout my (lentiviral) generation The first step in delivering genes via lentivirus is deciding what...

...titering, testing, and providing lentiviral preps of select lentiviral vectors from the repository for you....plasmid DNA that you can use along with lentiviral packaging plasmids to create your own lentivirus stocks... easy process. The cloning capacity of lentiviral vectors (~8.5 kb of insert) and the reduced titer associated... can make lentivirus production frustrating - a researcher can complete an entire lentiviral production... in lentiviral format. Finally, while we can’t currently produce custom gRNA-containing lentivirus, we... make new lentiviral preps on your own, fear not - anytime you order ready-to-use lentivirus from Addgene...re very excited to provide ready-to-use CRISPR lentiviral preps to researchers across the globe. Why provide...

...efficiency. Lentiviral transduction Cas9 and gRNA can be present in a single lentiviral transfer vector or separate...editing? Do you want to deliver the components as DNA, or would mRNA or protein delivery be more suitable...need to deliver a donor plasmid or single-stranded DNA oligonucleotide, so you will need to make sure your...transduction or injection as either protein, RNA, or DNA. Finally, once you have identified the best expression...Components of System Application Mammalian expression vector Promoter driving Cas9 expression can be constitutive...separate transfer vectors. May contain reporter gene (e.g. GFP) to identify and enrich positive cells....plasmids provide the necessary components to make lentiviral particles. Stable, tunable expression of Cas9...

...choosing AAV or lentiviral vectors in our previous blog posts.  Viral vector injection Viral vectors allow a ...required. On the other hand, lentiviral vectors are superior packaging vectors for larger genes - they can...around 1012-1013 vg/ml (vector genomes per milliliter) and lentiviral vectors around 108 IU/ml (infectious... region of interest. AAV or lentivirus?  Both AAV and lentiviral vectors are used for intracerebral injections...is cloned into a viral shRNA expression vector and the vector particles are delivered into the brain region...astrocytes, and AAV4 targets mostly astrocytes. Lentiviral vectors are pseudotyped in most cases with VSV-G ...using viral vectors By modulating neuronal gene expression and activity, you can use viral vectors to study...

... viral vector delivery methods. Lentivirus has limited packaging options, with most lentiviral vectors... screens rely on lentiviral systems, a fact supported by the wide array of lentiviral-based CRISPR libraries...packaging capacity and stable expression of lentiviral vectors make them an attractive option. However, ...B. (2019). High levels of AAV vector integration into CRISPR-induced DNA breaks. Nature Communications... screens. AAV vectors can be made at high titers equal to those achieved with lentivirus but offer an ... particularly in vivo ones. In those cases, AAV vector screens may be AAVantageous!       In vivo, in ...psuedotypes to further improve the tropism of AAV vectors, enhancing their affinity for specific cell and...

... system from a lentiviral vector.  Lentiviruses carry a (+) strand RNA copy of the DNA sequence, which... Multiplexed Lentiviral Expression Cassettes lentiviral transduction One vector expresses Cas9, ...expression vectors which are often more difficult to transfect or package into viral vectors. Cas9 multiplexing...streamlined vectors which are easier to use across multiple expression platforms: transfection, lentiviral transduction... 1) Most rely on transfection of more than one vector to express the gRNAs and Cas9. Co-transfections...multiplexing vectors are larger because they require regulatory sequences to allow for expression of multiple...Yamamoto Lab Golden Gate Assembly transfection One vector expresses Cas9 and up to 7 gRNAs Each gRNA requires...

...with pcDNA3.1-MS2-Tet1-CD to decrease methylation and activate transcription. A lentiviral vector with...covalent modifications to DNA or histones, the proteins that help package DNA, were associated with gene...initiates cytosine demethylation of DNA. However, several proteins in the DNA oxidation and repair pathways ...BioRender.com. DNA Methyltransferase MQ1 Margaret Goodell's lab has deposited pcDNA3.1-dCas9-MQ1(Q147L...gene expression or silencing. To alter DNA modifications, researchers used blunt tools like histone deacetylases...epigenetic modifier could be targeted to specific DNA sequences. Non-editing CRISPR applications direct...pLV-dCas9-p300-P2A-PuroR for lentiviral expression. Figure 1: dCas9-p300 adds H3K27ac marks to ...

...? Check out Addgene's Lentiviral dCas9 SAM vector and Piggybac dCas9 SAM vector! On/off control all the...Check out Addgene's Lentiviral dTAG cloning vector and SMASh degron tagging vector! Destabilizing domains...knock out plasmids? Check out our Cre vector and Murine LoxP vector.   Turn it off for a little while Transient...? Check out our Retro expression vector and Lenti expression vector. Turn it on for just a little while... Check out Addgene's Lentiviral Tet-on and AAVS1 targeted Tet-on transgene vector. Degron tags Tagging...will inhibit transcription instead of introducing a DNA double strand break. Transient introduction of CRISPRi...levels. Looking for a DD? Check out our DD-Cas9 vector! The price of tunable control These systems can...

... essential ingredients: the lentiviral packaging vector, a transfer vector including the gene of interest... 3rd-generation vectors? What does this mean for transfer vectors? A4: The lentiviral packaging systems...Addgene's most popular lentiviral plasmids Overview of Lentiviral Packaging Plasmids Lentiviral Protocols and ...clarification on these vectors. Read on to find the answers to our most frequently asked lentiviral questions. ... Depositor (and lentiviral expert) Didier Trono's lab webpage explains how lentiviral particles are produced...plasmids, an envelope plasmid, and the lentiviral transfer vector), rather than the three used for 2nd-... are different. As a result, lentiviral and retroviral packaging vectors are not interchangeable. While...

...use a virus? Viruses are essentially DNA (or RNA) delivery vectors, so what makes them better than the ...Cronin J, et al. “Altering the tropism of lentiviral vectors through pseudotyping.” Curr. Gene. Ther. ...from Your Lentiviral Transduction Additional Resources on Addgene.org Browse Our Viral Vector Pages Learn...Learn About Viral Vector Biosafety Deposit Your Viral Vectors ... cycle. Depending on the type of virus used, the DNA (or RNA) cargo will either permanently integrate ...infect cells with a high efficiency and deposit a DNA/RNA cargo into their targets makes them attractive...measures in a separate, upcoming post. If a viral vector seems like the right approach for your experiment...

...cloned all the cDNAs into a single consensus lentiviral expression vector and attached a DNA barcode to each...the appropriate cDNAs from other researchers, and cloned them into lentiviral vectors to generate a kit...identified a set of one to three mutant complementary DNAs (cDNAs) representing core nodes that, when over-expressed...

...Addgene blog Viral Vector Elements The Retroviral Life Cycle Popular Retroviral Vectors and Their Use in...about γ-retroviral vectors How do engineered γ-retroviruses differ from lentiviruses? There are a few important... well as the viral LTRs, differ. As a result, lentiviral and γ-retroviral packaging plasmids are not interchangeable...using 293T cells This method is very similar to lentiviral packaging methods. The three components described... polypurine tract; recognition site for proviral DNA synthesis. Increases transduction efficiency and ...found within both the 5' and 3' LTRs of retro/lentiviral plasmids. U5; Unique 5'; region at the 5' end...was originally published in 2018, in the Viral Vectors 101 eBook, 1st edition.  References and resources...

...use of this vector. A.2 Map of pLKO.1 pLKO.1 is a replication-incompetent lentiviral vector chosen by the...TRC-cloning vector with AgeI. Mix: 6 μg pLKO.1 TRC-cloning vector (maxiprep or miniprep DNA) 5 μL 10x NEB...TRC-Cloning Vector Click here to see the sequence of pLKO.1 TRC-cloning vector. The vector is 8901 base...Producing Lentiviral Particles E.1 Recommended materials E.2 Protocol for producing lentiviral particles...-Cloning Vector I.2 Recipes I.3 Warranty information Back to Top A. pLKO.1-TRC Cloning Vector A.1 The ...TRC-cloning vector. Addgene Plasmid Description pLKO.1 – TRC control Negative control vector containing... pLKO derived vectors that may also be useful for your experiment. To see these vectors, visit Addgene...

...heterogeneous population of gRNA-containing lentiviral transfer vectors, each targeting a specific gene within...synthesized. Pooled gRNAs are then cloned into lentiviral transfer vectors to create the CRISPR library. CRISPR ...use lentivirus to deliver a pooled population of gRNAs to Cas9 expressing cells. Pooled lentiviral CRISPR...gRNA generates a double-strand break in the target DNA, which, when repaired through error-prone non-homologous...amplification. If you've obtained a ready-to-use lentiviral preparation from Addgene, you can skip the steps...publication “Practical Considerations for Using Pooled Lentiviral CRISPR Libraries” (McDade et al., 2016). To learn...2016) Practical Considerations for Using Pooled Lentiviral CRISPR Libraries. Current Protocols in Molecular...

... existing transposon and vector systems and creating an all-synthetic vector that included only the elements... elements. DNA sequences for a desired component are cloned into Golden Gate entry vectors via traditional... into a destination vector in a predefined order. Finally, the destination vectors are used with Multisite... eight entry vectors can be used for each Multisite Gateway compatible destination vector for a maximum...deposited to Addgene, including lentiviral and AAV expression vectors for certain variants. Klapoetke...recognition sites that flank a DNA module in an inverse orientation, so that multiple DNA components can directionally...step, compatible Level 0 vectors are directionally assembled into a Level 1 vector creating a single transcriptional...

...with GeneWeld vectors GeneWeld from Jeffrey Essner’s lab uses CRISPR/Cas9 and a donor vector to create knock-ins...Jennifer Doudna’s lab discovered a new Cas protein, CasX, from metagenomic analysis of microbial DNA from ... tools for your research! This time: GeneWeld vectors to create knock-ins CasX Drug inducible CRISPR/...In this system, Cas9 generates a double-stranded DNA break at the genomic loci of interest and on either...cargo (ex: fluorescent protein gene) on the donor vector. Therefore, targeting requires two sgRNAS: one ...site and a universal sgRNA that targets the donor vector sequence. Homology arms that flank the donor plasmid... Read the paper in bioRxiv Find the GeneWeld vector kit CasX, a small Cas protein, is active in E....

...categories (e.g., for a lentiviral vector to be used in mammalian cells, check Lentiviral and Mammalian Expression...the vector backbone you cloned your insert into and fill out the appropriate boxes with the vector backbone...backbone manufacturer, vector backbone size, total size of your vector + insert, any modifications you ...Pooled libraries are normally supplied as liquid DNA with all of the plasmids in the library in a single...the help center article, "Can I deposit a pooled DNA library with Addgene?" or contact us at [email protected] any full or partial plasmid sequences and any vector maps. The more sequence data available, the better... was a part of the insert (when cloned into the vector backbone). If your plasmid has more than one insert...

...biosensor affinity for calcium.”  Primary Vector Type The vector type should describe how your plasmid ...indicate type in “Vector Type Other” column. For example, you may enter “donor template vector”. Cloning...deposit kit) with instructions on how to send liquid DNA or bacterial streaks of your plasmids back to us....: mammalian expression, bacterial expression, lentiviral, retroviral, AAV, RNAi, luciferase, cre/lox, .... Choose “high copy” if a sufficient quantity of DNA is produced from a miniprep and “low copy” if special... (prone to recombination in bacteria) such as lentiviral, retroviral, and AAV plasmids, choose the NEB...plasmids containing the ccdB gene, such as Gateway vectors, choose the ccdB Survival strain. For plasmids ...

...Multiple Lentiviral Expression Systems (MuLE) Kit: This kit can be used to create lentiviral vectors expressing...into distinct expression vectors using the enzyme BbsI. These expression vectors each contain Type IIS restriction...Cas9 or dCas9-containing destination vectors. Destination vector options include humanized wt Cas9, dCas9...wt hCas9 entry vectors are supplied with the kit, you can clone your own entry vectors containing other...containing vector using Golden Gate assembly, and up to 8 gRNAs may be expressed simultaneously. Vectors for ...Cas9-based genome engineering from a single lentiviral vector.” Nucleic Acids Research 42(19) (2014): e147...to express multiple gRNA from a single construct. DNA and gRNA are not to scale.   Let’s start with...

...create a final lentiviral vector containing up to 5 different elements. Lentiviral vectors constructed with...science.aaa3380 MuLE vectors for customized lentivirus production Finding a lentiviral vector backbone with ...expression vector which gives close to 100% cloning efficiency and gene expression. The pUCXKT vector ensures...custom-cloned vectors in a Multisite Gateway reaction with a desired destination vector expressing a separate...combinations of genetic elements in a single lentiviral vector provides a powerful tool to modify mammalian...routes in various conditions. The plasmids and lentiviral vectors encoding these proteins can also be used ...MXS-chaining kit contains 5 empty chaining vectors plus a destination vector and a collection of building blocks...

... the lentiviral vector, harvesting cells several days later, and isolating genomic DNA. This DNA is then...for in our reported titers. Lentiviral vectors are titered using a lentiviral droplet digital PCR (ddPCR...with serial dilutions of a lentiviral vector, incubated for 72 h, and genomic DNA is extracted. Primers and... . Browse our AAV vectors collection. AAV Data Hub Lentivirus Production Lentiviral preparations are produced... with viral vector manufacturing facilities, such as the University of Pennsylvania Vector Core (Link ...Pennsylvania Vector Core. In general, titering is performed by the facility that produced the viral vector lot....Empty vector particles can be identified after negative staining and appear darker than full vector particles...

...Viral Vectors 101: Viral Vector Elements Viral Vectors 101: An Introduction to AAV Viral Vectors 101:...synthesize complementary DNA copies of their genome upon infecting a host cell. This viral DNA (also called proviral...-studied lentivirus is HIV, which many lentiviral tools have been derived from. Lentiviruses have slightly...proviral DNA) is then inserted into the host's genome and is used to make more virus. The most studied ...Adeno-associated virus (AAV) is a small, single-stranded DNA parvovirus. AAV can infect humans and some other ...can replicate and divide along with the endogenous DNA. AAV also has low immunogenicity, meaning it does...Adenovirus Adenovirus has a linear, double stranded DNA genome which is non-enveloped. It can infect humans...

...(the most popular form), this viral vector is used to deliver DNA encoding Cre recombinase under the control... and other viral vector tools in the years to come. We’ll make many more viral vector tools and resources... Service Page Browse Additional Viral Vectors Find Viral Vector Protocols ... and requests are still pouring in! While our lentiviral service is going strong, the AAV service has ...be sure to check back often! To find more viral vectors available through Addgene, be sure to check out...out our viral service page and browse our viral vector blog posts for many more tips and ideas on how to...thanks to Hongkui Zeng’s lab for providing this AAV vector! Additional Resources AAV Titers: Where do they...

...consists of a pool of lentiviral vectors with roughly 73 million semi-random, 30 bp DNA barcodes that are ...individually label millions of mammalian cells through lentiviral infection and to monitor their abundance and ...optimize protocols for in vivo screening with lentiviral pooled shRNA libraries. Later, Bhang says, “When... Find the ClonTracer Library at Addgene Find Lentiviral Plasmids at Addgene Check out Our Cancer Resources...infection. When using the ClonTracer system, lentiviruses containing these barcodes infect the target ...

...infecting cells with sgRNAiBAR library containing lentiviral vectors the iBARs can be detected by next generation... as a DNA staining dye Article contributed by Jennifer Tsang Figure 4: Double stranded DNA bound... fusions with tTALE to “stain” DNA. TALE proteins can bind specific DNA sequences which brings the attached...4X CDRE repeat. In addition to showing that this vector is suitable for flow cytometry and fluorescence...alternative to intercalating fluorescent dyes for DNA-protein interaction studies? Kyubong Jo's lab generated...attached fluorescent protein to the DNA. They created plasmids for purification of tTALE-eGFP and tTALE-mCherry...intercalating fluorescent dyes which can cause photo-induced DNA breakage and structural deformation, these probes...