...is the tetracycline (Tet)-regulated system. You can choose either the Tet-off system or the Tet-on system... interest (pTet-IRES-EGFP, pPRIME-Tet-GFP-FF3) and one with either tTA or rtTA (FUW-M2rtTA). In this kind...system. In the Tet-off system a Tet-response element (TRE) is placed upstream of the promoter in your transfer...expression. In contrast, in the Tet-on system, tTA has been modified (and renamed rtTA) to bind TRE only in presence...expression kinetics as compared to the Tet-off system. One drawback of the Tet system is that it often requires... In the absence of tetracycline or a derivative such as Doxycycline (Dox), the Tet-controlled transactivator...vector systems containing both your transgene and tTA/rtTA (pINDUCER20, pINDUCER21) have been developed, but...

...lentivirus for delivering control shRNAs, GFP, and rtTA from the Tet-on system. The first two can serve as controls...experiments, while rtTA can be used to activate expression of a gene under the control of the tet operator. AAV...

...Collections Tetracycline (Tet) Inducible Expression Tetracycline (Tet) Inducible Expression Additional Resources...Plasmid Description Co-expressed tTA, rtTA, or TetR On or Off PI 21916 Tet-pLKO-neo 3rd generation lentiviral...Bujard tested the tet system in a mammalian cell system (HeLa) and found that the tet system was functional...cassette in format: PGK-rtTA-2A-puro; see article for tagged insert options rtTA On Root 11651 pLVUT-tTR-KRAB... binding of tTA or rtTA None Either Vogelstein 100521 pCW57.1-MAT2A Lentiviral Tet-Off all in one plasmid...interest with Strep-Tag and Tet-On 3G transactivator, creating an auto-regulated Tet-On 3G System. Contains...make cell lines Tet-On Advanced; Plasmid 26730 has hygro resistance 3rd generation rtTA On Campeau 25434...

...inducible promoters. The positive inducible tetracycline ON (Tet-On) system, a versatile tool developed for...activation. In this system, the activator rtTA (reverse tetracycline-controlled transactivator) is normally...Addgene.org: Inducible Promoters for Bacteria Tetracycline (Tet) On Expression Systems at Addgene Cre-lox ...lot. If you’re looking for high inducibility, the Tet-On system may be a good choice, as it’s documented...require only the addition of an external inducer, like Tet systems, may be activated very rapidly. In contrast...and cannot bind the tetracycline response elements (TRE) in a promoter. Tetracycline and its derivatives...

...transposase. In rtTA-cell lines, it is better to first engineer the cell line with rtTA, then integrate...directly in transient transfection in rtTA+ cell lines (such as HEK293-TETOn cells), or they can also be integrated...

...14749 for Thy1.1 selection. Benoist and Mathis 21915 Tet-pLKO-puro 3rd inducible expression of shRNA; puromycin...EF-1a-driven GFP and shRNA under the control of a tet-responsive H1 promoter Trono 11651 pLVUT-tTR-KRAB...cDNA. Puro selection. Shih 25737 pSLIK-Hygro 3rd Tet-based inducible shRNA or cDNA expression, gateway... other versions of pSLIK. Fraser 15948 pLOVE 3rd Tet inducible gateway destination plasmid for cDNA expression...versions of this plasmid. Root 44012 pInducer20 3rd Tet-inducible lentivirus for ORF expression, multicistronic...See plasmid 21374 for dtomato version. Welm 21916 Tet-pLKO-neo 3rd inducible expression of shRNA; neomycin...Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-puro. See article for more versions of this plasmid...

...Vignali 27995 TtRMPVIR CMV/MSV Tet-regulated expression of shRNA; expresses rtTA. See Zuber et al. 2011 for...resistance. Hahn 63704 pRetroX GFP T2A Cre CMV/MSV Tetracycline inducible expression of GFP T2A Cre fusion in...

...recombinases (Cre, Flp etc.), and tetracycline drug induction systems (ttA or rtTA) are between 1 to 2 kb in ...

...has used an inducible MitoTimer reporter using a Tet-on system (pTRE-Tight-MitoTimer) to show its usefulness...cells stably expressing pTRE-Tight-MitoTimer and rtTA. The cells were pulsed with 2µg/ml of Doxycycline...repressor plasmids from Scot Wolfe's lab. These include Tet-inducible CRISPR activators and repressor plasmids...

... pBS537 tet-hCMV-GFPcre tet inducible Cre-GFP fusion tet-hCMV Mammalian Sauer 11961 pBS596 tet-hCMV-GFPcre...none Mammalian Sauer 11957 pBS595 tet-hCMV-EGFPcre Cre-EGFP fusion Tet inducible Mammalian Sauer 11958 ...Supernova TRE AAV Iwasato 85577 pTC-CMV-Tet CreER expression and tetracyclin-dependent transgene/shRNA expression...ApoE.HCR.hAAT Mammalian Ehmer 85578 pTC-ApoE-Tet CreER expression and tetracyclin-dependent transgene/shRNA expression...tet-hCMV-GFPcre tet inducible Cre-GFP fusion, metallothionein MT-I region including the polyadenylation site and...and several introns tet-hCMV Mammalian Sauer 12168 pMB80 (R26-CreER) Cre-ERT2 with loxp cassette; Targeting...Mammalian Messing 45359 pNK-TGCK Cre-EGFP fusion; Tet inducible - rrTA expression driven by mouse Nkx cardiac...