-
Plasmid#41553DepositorInsertASC (PYCARD Human)
UseTagsHAExpressionMammalianMutationPromoterCMV immediate-early enhancer/promoterAvailable sinceDec. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRP_ASC-LmCerulean
Plasmid#41840DepositorInsertapoptosis-associated speck-like protein containing CARD (PYCARD Human)
UseRetroviralTagsmCeruleanExpressionMammalianMutationStop Codon removedPromoterCMVAvailable sinceJuly 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Myc-ASC
Plasmid#73952PurposeExpress c-Myc-tagged human ASC in mammalian cellsDepositorInsertPYD and CARD domain containing, PYCARD (PYCARD Human)
UseTagsc-mycExpressionMammalianMutationPromoterCMVAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ gfp-zASC
Plasmid#98703PurposeGFP tagged zebrafish ASC in the pCS2+ vector for in vitro transcriptionDepositorInsertApoptosis associated speck like protein containing CARD (pycard Zebrafish)
UseZebrafish/xenopus expressionTagsGFPExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLEX-MCS-ASC-GFP
Plasmid#73957PurposeExpress human ASC with a C-terminal EGFP tag in mammalian cellsDepositorInsertPYD and CARD domain containing, PYCARD (PYCARD Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterCMV IEAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-N-Flag-ASC1
Plasmid#75134PurposeExpresses N-terminal Flag-tagged ASC1 in mammalian cellsDepositorInsertPYD and CARD domain containing (Pycard Mouse)
UseTagsFLAGExpressionMammalianMutationF23S (please see depositor comments below)PromoterCMVAvailable sinceAug. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLEX307 ASC
Plasmid#163977PurposeMammalian expression vector encoding ASC (for example, to assess ASC-speck formation or inflammasome signalling assays)DepositorInsertASC (PYCARD Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
ASC-CASP1 Octamer
Plasmid#164032PurposeBacterial expression vector encoding a 5GSS-linked ASC(CARD)-CASP1(CARD) construct to express an octamerDepositorUseTagsHis6-MBP-TEVExpressionBacterialMutationW169G (ASC), G20K (CASP1)PromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only