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Plasmid#212195PurposeBacterial expression plasmid for production of HIV p24 protein with a polyhistidine tag at the N-terminusDepositorInsertChain A, Capsid protein p24, HIV type 1
UseTagsPolyhistidineExpressionBacterialMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJUMP18-Histag_C
Plasmid#127035PurposeBasic Part C- C-terminus; polyhistidine tagDepositorInsertPart His6_C
UseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJuly 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJUMP18-Histag_N
Plasmid#127033PurposeBasic Part N- N-terminus; polyhistidine tagDepositorInsertPart His6_N
UseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJuly 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
rFIXX-bp chain A (IRESneo)
Plasmid#82450PurposeChain A of the habu snake venom FIX/FX binding protein with a C-terminal polyhistidine-tag. Cloned into pIRESneo3 (Ampicillin) via 5' NheI and 3' BamHI; IRES vector conferring neomycin resistance.DepositorInsertrFIXX-bp chain A
UseTagsPolyhistidineExpressionBacterial and MammalianMutationPromoterT7 RNA polymerase promoterAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET15b PKA Cat
Plasmid#14921DepositorInsertPKA catalytic subunit alpha (Prkaca Mouse)
UseTags6xHis and Thrombin cleavage siteExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2007AvailabilityAcademic Institutions and Nonprofits only -
pETSG
Plasmid#159418PurposeExpression of proteins with a C-terminal thermostable GFP (TGP) tag in Escherichia coliDepositorInsertthermostable GFP (TGP)
UseTagspolyhistidine (poly-His)ExpressionBacterialMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBTSG
Plasmid#159420PurposeTransient expression of proteins with a C-terminal thermostable GFP (TGP) tag in mammalian cellsDepositorInsertthermostable GFP (TGP)
UseTagspolyhistidine (poly-His)ExpressionMammalianMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYTSG
Plasmid#159419PurposeExpression of proteins with a C-terminal thermostable GFP (TGP) tag in Saccharomyces cerevisiaeDepositorInsertthermostable GFP (TGP)
UseTagspolyhistidine (poly-His)ExpressionYeastMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBP-HexHis
Plasmid#72983PurposeHexahistidine N-terminal tag (5' TAAA/3' CATA fusion)DepositorInsertPolyhistidine (HHHHHH) N-terminal tag
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
LI D-E 6xHIS
Plasmid#54447DepositorInsertpolyhistidine (6x) tag
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceJune 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
LI B-C 6xHIS
Plasmid#54446DepositorInsertpolyhistidine (6x) tag
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceJune 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAGM1479
Plasmid#50291DepositorInsert5'UTR, omega (Tobacco Mosaic Virus) + N terminal HIS tag (6x polyhistidine)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pWPI/hPLK2WT/Neo
Plasmid#35385DepositorInserthPLK2 (PLK2 Human)
UseLentiviralTagsEMCV IRES-Neomycin cassette and V5 epitope and Po…ExpressionMammalianMutationPromoterEF1-alphaAvailable sinceApril 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pWPI/hPLK2K111M/Neo
Plasmid#35386DepositorInserthPLK2 K111M (PLK2 Human)
UseLentiviralTagsEMCV IRES-Neomycin cassette and V5 epitope and Po…ExpressionMammalianMutationK111MPromoterEF1-alphaAvailable sinceApril 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMBS-849
Plasmid#218004Purpose8xHis-tag fused to PreScission Protease cleavage site for protein purification and cleavageDepositorInsert8xHis-tag
UseSynthetic BiologyTagsPreScission protease cleavage siteExpressionMutationPromoterAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-MHL.SUZ12
Plasmid#125172PurposeExpresses human SUZ12 in insect cells, under a TEV-cleavable N-terminal polyhistidine tag.DepositorInsertSUZ12 (SUZ12 Human)
UseTagsHis-TEVExpressionInsectMutationPromoterPolyhedrinAvailable sinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET26b(+)-ATI_QconCAT_1
Plasmid#163957PurposeExression of ATI QconCAT protein in E. coli expression strains (BL21). Encodes a concatemer of tryptic peptides from wheat amylase/trypsin inhibitors. Used as standard for LC-MS-based quantification.DepositorInsertATI QconCAT
UseTagspolyhistidine tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-MHL.RBBP4
Plasmid#125173PurposeExpresses human RBBP4 in insect cells, under a TEV-cleavable N-terminal polyhistidine tag.DepositorInsertHistone-binding protein RBBP4 (RBBP4 Human)
UseTagsHis-TEV siteExpressionInsectMutationPromoterPolyhedrinAvailable sinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-MHL.EED
Plasmid#125174PurposeExpresses human EED in insect cells, under a TEV-cleavable N-terminal polyhistidine tag.DepositorInsertPolycomb protein EED (EED Human)
UseTagsHis-TEVExpressionInsectMutationPromoterPolyhedrinAvailable sinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-MHL.EZH2
Plasmid#125171PurposeExpresses human EZH2 in insect cells, under a TEV-cleavable N-terminal polyhistidine tag.DepositorInsertEZH2 (EZH2 Human)
UseTagsHis-TEVExpressionInsectMutationPromoterPolyhedrinAvailable sinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only