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Plasmid#87853PurposeThis plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.DepositorInsertTPNOX
UseTagsC-terminal FLAG tag with a linkerExpressionBacterialMutationPromoterAvailable sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC57-mitoLbNOX
Plasmid#74448PurposeThis plasmid contains human codon optimized sequence of mitoLbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.DepositorInsertmitoLbNOX
UseTagsFlag tag with linker and mitochondrial targeting …ExpressionMutationHuman codon optimizedPromoterAvailable sinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC57-LbNOX
Plasmid#75285PurposeThis plasmid contains human codon optimized sequence of LbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.DepositorInsertLbNOX
UseTagsC-terminal FLAG tag with linkerExpressionMutationHuman codon optimizedPromoterAvailable sinceMay 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC57-mitoTPNOX
Plasmid#87854PurposeThis plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.DepositorInsertmitoTPNOX
UseTagsC-terminal FLAG tag with a linker and mitochondri…ExpressionBacterialMutationPromoterAvailable sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC57-CmYLCV
Plasmid#205245PurposeFor plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertCmYLCV-csy4_binding_site-esgRNA-tevopreQ1-csy4_binding_site
UseCRISPRTagsExpressionPlantMutationPromoterCmYLCVAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC57 (Gent)
Plasmid#54338DepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoterAvailable sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Curlcake3
Plasmid#139342PurposeExpresses Curlcake3 (CC3) in E.coliDepositorInsertCurlcake3 with T7 promoter
UseTagsExpressionBacterialMutationPromoterT7Available sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Curlcake1
Plasmid#139340PurposeExpresses Curlcake1 (CC1) in E.coliDepositorInsertCurlcake1 with T7 promoter
UseTagsExpressionBacterialMutationPromoterT7Available sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Curlcake2
Plasmid#139341PurposeExpresses Curlcake2 (CC2) in E.coliDepositorInsertCurlcake2 with T7 promoter
UseTagsExpressionBacterialMutationPromoterT7Available sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Curlcake4
Plasmid#139343PurposeExpresses Curlcake4 (CC4) in E.coliDepositorInsertCurlcake4 with T7 promoter
UseTagsExpressionBacterialMutationPromoterT7Available sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC57-PP7v3
Plasmid#107230PurposeExpresses 24x optimized PP7 stem loops (version 3) in bacterial cellsDepositorInsert24xPP7v3
UseTags3x FLAG-tag and P2A siteExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC57 APEX
Plasmid#40306PurposeA monomeric and improved-activity variant of pea ascorbate peroxidase. Used for electron microscopy and live-cell proteomic mapping. This construct has been replaced by APEX2 (construct 49386).DepositorInsertpea APEX (Synthetic)
UseUnspecifiedTagsExpressionMutationK14D, W41F, E112K (relative to wild-type)PromoterAvailable sinceSept. 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Kan_US71_APikL1
Plasmid#123646PurposeLevel 0 Golden-Gate compatible vectorDepositorInsertUS71_APikL1
UseGolden gateTagsExpressionMutationPromoterAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC57-R3R2
Plasmid#105109Purposecreate your own 2in1 constructsDepositorTypeEmpty backboneUseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC57-R1R4
Plasmid#105110Purposecreate your own 2in1 constructsDepositorTypeEmpty backboneUseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC57-4eCOL2A1
Plasmid#97211PurposeFor cloning of a COL2A1-based, chondrogenesis-responsive promoter, and subcloning this insert into new vectorsDepositorInsert4 repeats of COL2A1 intronic enhancer (+2126/+2174) upstream of core promoter (-164/+37) (COL2A1 Human)
UseVector is designed for cloning and generation of …TagsExpressionMutationPromoterCOL2A1 regulatory elementsAvailable sinceOct. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC57 moxDendra2
Plasmid#89791Purposebacterial expression of moxDendra2DepositorInsertmoxDendra2
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Kan_BR29_00091361
Plasmid#121209PurposeLevel 0 Golden-Gate compatible vectorDepositorInsertBR29_00091361
UseGolden gateTagsExpressionMutationMagnaporthe griseaPromoterAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Kan_FR13_APikL2
Plasmid#123648PurposeLevel 0 Golden-Gate compatible vectorDepositorInsertFR13_APikL2
UseGolden gateTagsExpressionMutationPromoterAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC57-8xHis
Plasmid#101699Purpose8xHis in domestication vectorDepositorInsertAATG-[8xHis]-AGCC
UseYeast expressionTagsExpressionMutationPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only