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Plasmid#73794Purposeratiometric pHluorin2 sequence in a middle entry cloneDepositorInsertratiometric pHluorin2
UseMiddle entry cloneTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
RpHLuorin2
Plasmid#180227Purposefor bacterial expression of recombinant ratiometric pHluorin2DepositorInsertRatiometric pHLuorin2
UseTagsHis-tag followed by thrombin cleavage siteExpressionBacterialMutationPromoterT7Available sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
ER-RpHLuorin2
Plasmid#171717PurposeLuminal ER expression of ratiometric pHLuorin2DepositorInsertRatiometric pHLuorin2 targeted to ER
UseTagsKDEL ER-retention signal and Signal sequence of h…ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRpHLuorin2-N1
Plasmid#171716PurposeRatiometric pHLuorin2 backbone (C terminal fusion)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
LAMP1-RpHLuorin2
Plasmid#171720PurposeLysosomal expression of ratiometric pHLuorin2DepositorInsertRatiometric pHLuorin2 fused to LAMP1 (LAMP1 Human)
UseTagsratiometric pHluorin2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
GalT-RpHLuorin2
Plasmid#171719PurposeTrans-Golgi expression of ratiometric pHLuorin2DepositorInsertRatiometric pHLuorin2 fused to B4GALT1 (B4GALT1 Human)
UseTagsratiometric pHluorin2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
MGAT2-RpHLuorin2
Plasmid#171718PurposeCis-/medial-Golgi expression of ratiometric pHLuorin2DepositorInsertRatiometric pHLuorin2 fused to MGAT2 (MGAT2 Human)
UseTagsratiometric pHluorin2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
GPI-RpHLuorin2
Plasmid#171721PurposeGPI-anchored RpHLuorin2 for plasma membrane expression (extracellular)DepositorInsertRatiometric pHLuorin2 fused to GPI anchor
UseTagsratiometric pHluorin2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pME phluorin2-gpi
Plasmid#73793Purposeratiometric pHluorin2 with a gpi linkage sequence in a middle entry cloneDepositorInsertratiometric pHluorin2-gpi
UseMiddle entry cloneTagsExpressionMutationPromoterAvailable sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLEW-pHluorin2-PTS1
Plasmid#213776PurposeExpresses the fluorescent protein pH sensor pHlorin2 in the glycosomes of Trypanosomal bruceiDepositorInsertpHlorin2
UseTrypanosoma brucei expressionTagsExpressionMutationAppended a 2 glycine linker and a T. brucei perox…PromoterT. brucei rRNA promotorAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_TNF-SBP-RpHLuorin2
Plasmid#171722PurposeSynchronize trafficking of TNFa-RpHLuorin2DepositorInsertTNF (TNF Human)
UseTagsStreptavidin Binding Protein (SBP) and ratiometri…ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only