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pcDNA3-Flag-NSF

Plasmid

#74924

Purpose

Mammalian expression of human NSF full-length (744 aa)

Depositor

Elisa Greggio

Article

Belluzzi et al Mol Neurodegener. 2016 Jan 13;11(1):1. d

Insert

NSF (NSF Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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pHAGE-EGFR-A763_Y764insFQEA

Plasmid

#116233

Purpose

Lentiviral expression of EGFR A763_Y764insFQEA

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR (EGFR Human)

Use

Lentiviral

Tags

Expression

Mutation

A763_Y764insFQEA

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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Lactotransferrin-CD4d3+4-bio

Plasmid

#73124

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding Lactotransferrin with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

Lactotransferrin (LTF Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF18-C1878_02750_GsVadh(Gs28C)

Plasmid

#225304

Purpose

Expresses Gordonibacter sp. 28C Gs Vadh in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Gs Vadh

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-NSF/S647A

Plasmid

#74938

Purpose

Mammalian expression of human NSF mutant S647A (mutation in the D2 domain of the protein)

Depositor

Elisa Greggio

Article

Belluzzi et al Mol Neurodegener. 2016 Jan 13;11(1):1. d

Insert

NSF (NSF Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S647A (mutation in the D2 domain of the protein)

Promoter

CMV

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pXD80ENSF7.2-Dadh-ΔElen0470(ElA2)

Plasmid

#225317

Purpose

Expresses Eggerthella lenta A2 Dadh (ΔElen_0470) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh (ΔElen_0470)

Use

Tags

His8

Expression

Bacterial

Mutation

Promoter

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT156: pHelper(ZooTsy)_ΔTnsF

Plasmid

#200869

Purpose

Inducible expression of ZooTsy (except TnsF) proteins.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

ZooTsy

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT162: pTwinStrep-bdSUMO-AjTn6022_TnsF

Plasmid

#200865

Purpose

AjTn6022_TniQ expression construct for protein purification.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

AjTn6022_TnsF

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF7.3-Dadh-ΔElen0473(ElA2)

Plasmid

#225318

Purpose

Expresses Eggerthella lenta A2 Dadh (ΔElen_0473) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh (ΔElen_0473)

Use

Tags

His8

Expression

Bacterial

Mutation

Promoter

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF7.6-Dadh-ΔElen0476(ElA2)

Plasmid

#225321

Purpose

Expresses Eggerthella lenta A2 Dadh (ΔElen_0476) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh (ΔElen_0476)

Use

Tags

His8

Expression

Bacterial

Mutation

Promoter

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF7.1-Dadh-ΔElen0469(ElA2)

Plasmid

#225315

Purpose

Expresses Eggerthella lenta A2 Dadh (ΔElen_0469) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh (ΔElen_0469)

Use

Tags

His8

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF7.4-Dadh-ΔElen0474(ElA2)

Plasmid

#225319

Purpose

Expresses Eggerthella lenta A2 Dadh (ΔElen_0474) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh (ΔElen_0474)

Use

Tags

His8

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF7.7-Dadh-ΔElen0477(ElA2)

Plasmid

#225322

Purpose

Expresses Eggerthella lenta A2 Dadh (ΔElen_0477) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh (ΔElen_0477)

Use

Tags

His8

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF7.8-Dadh-ΔElen0478(ElA2)

Plasmid

#225323

Purpose

Expresses Eggerthella lenta A2 Dadh (ΔElen_0478) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh (ΔElen_0478)

Use

Tags

His8

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF7.9-Dadh-ΔElen0481(ElA2)

Plasmid

#225324

Purpose

Expresses Eggerthella lenta A2 Dadh (ΔElen_0481) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh (ΔElen_0481)

Use

Tags

His8

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF7.10-Dadh-ΔElen0482(ElA2)

Plasmid

#225325

Purpose

Expresses Eggerthella lenta A2 Dadh (ΔElen_0482) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh (ΔElen_0482)

Use

Tags

His8

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-NSF/T646A

Plasmid

#74937

Purpose

Mammalian expression of human NSF mutant T646A (mutation in the D2 domain of the protein)

Depositor

Elisa Greggio

Article

Belluzzi et al Mol Neurodegener. 2016 Jan 13;11(1):1. d

Insert

NSF (NSF Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

T646A (mutation in the D2 domain of the protein)

Promoter

CMV

Available since

April 26, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3-Flag-NSF/T645A

Plasmid

#74936

Purpose

Mammalian expression of human NSF mutant T645A (mutation in the D2 domain of the protein)

Depositor

Elisa Greggio

Article

Belluzzi et al Mol Neurodegener. 2016 Jan 13;11(1):1. d

Insert

NSF (NSF Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

T645A (mutation in the D2 domain of the protein)

Promoter

CMV

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pET co-transformation cloning vector (13S-A)

Plasmid

#48323

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF26-Pct3-(+)-Eldh1(nHis6)(GpDSM19378)

Plasmid

#233874

Purpose

Expresses His-tagged Gordonibacter pamelaeae DSM 19378 (+)-Eldh1 with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(+)-Eldh1 (GPA_25810 Gordonibacter pamelaeae DSM 19378)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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