Skip to main content
Addgene
Showing: 1 - 20 of 112478 results

  13. pAc5.1B-lambdaN-HA-DmPiwi_O

    Plasmid
    #147072
    Purpose
    Insect Expression of DmPiwi
    Depositor
    Elisa Izaurralde
    Insert
    DmPiwi (piwi Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    first 42 AA missing and two non silent mutations …
    Promoter
    Available since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    147072-plasmid-map-sequence-id-289519

    Enlarge

  16. pD649-HAsp-MPIG6B-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156504
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    MPIG6B (MPIG6B Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    Aug. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    156504-plasmid-map-sequence-id-368834

    Enlarge

  17. pOCC5

    Plasmid
    #118863
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C-NotI-ccdB-AscI-stop-HindIII cassette
    Use
    Tags
    HIS6, cleavable with 3C protease
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118863-plasmid-map-sequence-id-232276

    Enlarge

  18. pOCC7

    Plasmid
    #118876
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal HIS6 tag, cleavable with 3C
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    Use
    Tags
    HIS6, cleavable with 3C protease
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118876-plasmid-map-sequence-id-232237

    Enlarge

  19. pOCC65

    Plasmid
    #118865
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease, and N-terminal monomeric GFP
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C-mGFP-NotI-ccdB-AscI-stop-HindIII cassette
    Use
    Tags
    HIS6, cleavable with 3C protease, mGFP
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118865-plasmid-map-sequence-id-232317

    Enlarge

  20. pOCC292

    Plasmid
    #118881
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal mRuby3, and C-terminal HIS6 tag, cleavable with 3C
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-NotI-ccdB-AscI-mRuby3-3C-HIS6-stop-HindIII cassette
    Use
    Tags
    mRuby3, HIS6, cleavable with 3C protease
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118881-plasmid-map-sequence-id-232308

    Enlarge

Showing: 1 - 20 of 112478 results