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Showing: 1 - 20 of 7636 results

  1. SuperClomeleon

    Plasmid
    #203724
    Purpose
    An improved engineered fluorescent ratiometric optogenetic sensor to detect inhibitory synaptic activity via changes in intracellular chloride
    Depositor
    George Augustine
    Article
    Grimley et al J Neurosci. 2013 Oct 9;33(41):16297-309.
    Insert
    Cerulean linked topaz fluorescent protein
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Cerulean, modified Topaz Fluorescent Protein with…
    Promoter
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    203724-plasmid-map-sequence-id-403640

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  2. pThermeleon

    Plasmid
    #86120
    Purpose
    Bandpass temperature-gated expression of mWasabi and mCherry reporters
    Depositor
    Mikhail Shapiro
    Article
    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi
    Inserts
    TlpA
    mWasabi
    TcI38
    mCherry
    cI
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    A67T, M1V, L65S, K68R, F115L, D126G, D188G and D3…
    Promoter
    pR/pL, pR/pL, pLacI, and pTlpA
    Available since
    March 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    86120-plasmid-map-sequence-id-173201

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  3. MELOPS

    Plasmid
    #80151
    Purpose
    Genetically encoded melanosome-localized pH sensor.
    Depositor
    Santiago Di Pietro
    Article
    Ambrosio et al Proc Natl Acad Sci U S A. 2016 May 17;11
    Insert
    OCA2 (OCA2 Human)
    Use
    Tags
    mNectarine in first luminal loop
    Expression
    Mammalian
    Mutation
    nonpathologic polymorphism D257A, pore mutation V…
    Promoter
    CMV
    Available since
    Sept. 20, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits

  4. Clomeleon

    Plasmid
    #203723
    Purpose
    A genetically encoded ratiometric optical indicator for chloride ions, to detect changes in intracellular chloride concentration
    Depositor
    George Augustine
    Article
    Kuner et al Neuron. 2000 Sep;27(3):447-59. doi: 10.1
    Insert
    cyan fluorescent protein linked yellow florescent protein
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Cyan Fluorescent Protein, Topaz Fluorescent Prote…
    Promoter
    Available since
    Sept. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    203723-plasmid-map-sequence-id-403632

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  5. pDmelOR

    Plasmid
    #126476
    Purpose
    Vector for the functional expression of insect ORs in mammalian cells, with a human codon-optimized version of D.melanogaster Orco (hOrco) with an N-terminal myc tag and a β-globin/IgG chimeric intron
    Depositor
    Bill Hansson
    Article
    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.
    Insert
    DmelOrco (Orco Fly)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    codon optimization for H. sapiens; includes a rec…
    Promoter
    CMV
    Available since
    June 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    126476-plasmid-map-sequence-id-246523

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  14. pMEL15_MATz

    Plasmid
    #166099
    Purpose
    Expression of gRNA targeting the MAT locus
    Depositor
    Rebecca Burgess
    Insert
    MAT Z1
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166099-plasmid-map-sequence-id-328795

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  19. pFastBac1-melHis10TEV

    Plasmid
    #159428
    Purpose
    empty pFastBac1 secretion vector (polyhedrin promoter) adds N-term melittin signal sequence & TEV-protease-removable His10; avoids unwanted residues in final protein by using BseRI restriction sites
    Depositor
    Debra Hansen
    Type
    Empty backbone
    Use
    Tags
    melittin signal sequence (removed in vivo by sign…
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Available since
    June 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    159428-plasmid-map-sequence-id-333931

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  20. YC 3.6 Cameleon

    Plasmid
    #58182
    Purpose
    Localization: Cytosolic Ca Biosensor, Excitation: 433 / 515, Emission: 475 / 528
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    ECFP and Venus
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 28, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 1 - 20 of 7636 results