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Showing: 1 - 20 of 54 results

  1. IBMc396

    Plasmid
    #161954
    Purpose
    Demonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase N-lobe fused to acVHH
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSEVA321-PBAD(SapI-)-B30-His-T7RNAP(29-1)-15aa-acVHH-T
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD
    Available since
    March 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    161954-plasmid-map-sequence-id-328230

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  2. IDG_TMC3_OE_1

    Plasmid
    #161629
    Purpose
    Overexpresses TMC3
    Depositor
    Mike McManus
    Insert
    TMC3 (TMC3 Human)
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    161629-plasmid-map-sequence-id-319361

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  5. IBMc326

    Plasmid
    #161949
    Purpose
    Contains Type IIS restriction site free elements for expression vectors in IBM. Expression vector to generate ORF insertion library in IBM on ECF20
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-5)-BsaI-BsaI-ECF20(187-193)-Ter
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD
    Available since
    March 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    161949-plasmid-map-sequence-id-327545

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  6. PSMC3_miniTurbo_Nterm

    Plasmid
    #221525
    Purpose
    Flag-tagged biotinylation enzyme for co-expression of Psmc3
    Depositor
    Alessandro Ori
    Insert
    PSMC3 (PSMC3 Human)
    Use
    Tags
    miniTurbo
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    221525-plasmid-map-sequence-id-439440

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  7. PSMC3_BirA_Nterm

    Plasmid
    #221524
    Purpose
    Flag-tagged biotinylation enzyme for co-expression of Psmc3
    Depositor
    Alessandro Ori
    Insert
    PSMC3 (PSMC3 Human)
    Use
    Tags
    BirA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    221524-plasmid-map-sequence-id-439226

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  8. IBMc397

    Plasmid
    #161955
    Purpose
    Demonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase C-lobe fused to acVHH
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB3K3-TT-PBAD(SapI-)-B30-His-acVHH-15aa-T7RNAP(181+)-T
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD
    Available since
    Oct. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    161955-plasmid-map-sequence-id-326361

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  9. IBMc393

    Plasmid
    #161953
    Purpose
    Demonstrates chemically induced dimmerization of acVHH. T7 RNAP polymerase reporter plasmid
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB4A3-T-PT7-B30-mScarlet-I-3T
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PT7
    Available since
    March 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    161953-plasmid-map-sequence-id-327676

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  10. IBMc330

    Plasmid
    #161952
    Purpose
    Contains elements for building reporter plasmid for IBM. SrpR reporter plasmid
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSEVA171-PSrpR-B30-mScarlet-I-Ter
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PSrpR
    Available since
    March 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    161952-plasmid-map-sequence-id-327550

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  11. IBMc367

    Plasmid
    #161947
    Purpose
    Contains Type IIS restriction site free elements for IBM staging vectors. Additional BsaI site to facilitate size selection. Staging plasmid for IBM on ECF20
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB1C3(BsmBI-,BsaI+)-BsaI-ECF20(6-188)-BsaI_v3
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    161947-plasmid-map-sequence-id-326355

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  13. MC3R-Tango

    Plasmid
    #66429
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    MC3R (MC3R Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 1 - 20 of 54 results