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ER-mit RspA-splitFAST (lentiviral plasmid)

Plasmid

#233604

Purpose

Expression of ER-mit RspA-splitFAST by a single lentiviral plasmid encoding the two fragments

Depositor

Riccardo Filadi

Article

Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

Insert

RspA-splitFAST

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV/SV40

Available since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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hu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid

Plasmid

#154429

Purpose

hu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid

Depositor

David Liu

Article

Koblan et al Nature. 2021 Jan;589(7843):608-614. doi:

Insert

hu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

VRQR point mutations in SpCas9

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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Lentiviral click editor (CE1) plasmid - LTR-pEF1a-PCV2-nCas9-EcKlenow (DRR921)

Plasmid

#217798

Purpose

Lentiviral plasmid to produce the prototypical CE1 construct (PCV2-nCas9-EcKlenow), expressed from the EF1a promoter

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS

Use

CRISPR and Lentiviral

Tags

BPNLS

Expression

Mammalian

Mutation

nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

Promoter

EF1a

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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Lentiviral mBmal1-Luc

Plasmid

#182762

Purpose

Lentiviral vector encoding the promoter region of mouse Bmal1 gene driving a luciferase reporter

Depositor

Qing-Jun Meng

Article

Gossan et al Arthritis Rheum. 2013 Sep;65(9):2334-45.

Insert

mouse arntl promoter (Bmal1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

649 bp

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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Lentiviral-TOP-dGFP-reporter

Plasmid

#14715

Purpose

Beta-catenin reporter - 3rd generation self-inactivating lentiviral plasmid LEF-1/TCF-responsive promoter driven d2-eGFP (destabilized, half-life of 2h).

Depositor

Tannishtha Reya

Article

Reya et al Nature. 2003 May 22. 423(6938):409-14.

Insert

LEF-1/TCF-responsive promoter (Ctnnb1 )

Use

Lentiviral

Tags

d2 eGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only

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Synapsin sHRPa-NRX in FSW lentiviral vector

Plasmid

#73147

Purpose

Large fragment of split HRP fused to the extracellular terminus of neurexin3B. This is a lentiviral plasmid, but can also be used for transfections. Synapsin promoter.

Depositor

Alice Ting

Article

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

sHRPa-NRX

Use

Lentiviral

Tags

2x HA tag and Flag tag

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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Synapsin sHRPb-NLG in FSW lentiviral vector

Plasmid

#73148

Purpose

Small fragment of split HRP fused to the extracellular terminus of NLG1. This is a lentiviral plasmid, but can also be used for transfections. Syanpsin promoter.

Depositor

Alice Ting

Article

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

sHRPb-NLG (Nlgn1 Mouse, Synthetic)

Use

Lentiviral

Tags

V5 tag

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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MTK Lentivirus Multi Cassette Destination Vector (pAN2424)

Plasmid

#194221

Purpose

Backbone for constructing multi casette expression plasmids using the Mammalian Tookit

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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MTK Lentivirus Single Cassette Destination Vector (pAN2414)

Plasmid

#194220

Purpose

Backbone for constructing single casette expression plasmids using the Mammalian Tookit

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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RPS4X sgRNA-2 lentiCRISPR v2 plasmid

Plasmid

#192232

Purpose

lentiviral vector expressing sgRNA-2 targeting eIF3 binding site of RPS4X 5'UTR

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-2 targeting eIF3 binding site of RPS4X 5'UTR (RPS4X Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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RPS4X sgRNA-1 lentiCRISPR v2 plasmid

Plasmid

#192231

Purpose

lentiviral vector expressing sgRNA-1 targeting eIF3 binding site of RPS4X 5'UTR

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-1 targeting eIF3 binding site of RPS4X 5'UTR (RPS4X Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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RPS15A sgRNA-2 lentiCRISPR v2 plasmid

Plasmid

#192228

Purpose

lentiviral vector expressing sgRNA-2 targeting eIF3 binding site of RPS15A 5'UTR

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-2 targeting eIF3 binding site of RPS15A 5'UTR (RPS15A Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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RPS15A sgRNA-1 lentiCRISPR v2 plasmid

Plasmid

#192227

Purpose

lentiviral vector expressing sgRNA-1 targeting eIF3 binding site of RPS15A 5'UTR

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-1 targeting eIF3 binding site of RPS15A 5'UTR (RPS15A Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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RPS4X sgRNA-3 lentiCRISPR v2-Blast plasmid

Plasmid

#192233

Purpose

lentiviral vector expressing sgRNA-3 targeting eIF3 binding site of RPS4X 5'UTR

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-3 targeting eIF3 binding site of RPS4X 5'UTR (RPS4X Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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RPS15A sgRNA-5 lentiCRISPR v2-Blast plasmid

Plasmid

#192230

Purpose

lentiviral vector expressing sgRNA-5 targeting eIF3 binding site of RPS15A 5'UTR

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-5 targeting eIF3 binding site of RPS15A 5'UTR (RPS15A Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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RPS4X sgRNA-4 lentiCRISPR v2-Blast plasmid

Plasmid

#192234

Purpose

lentiviral vector expressing sgRNA-4 targeting eIF3 binding site of RPS4X 5'UTR

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-4 targeting eIF3 binding site of RPS4X 5'UTR (RPS4X Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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RPS15A sgRNA-4 lentiCRISPR v2-Blast plasmid

Plasmid

#192229

Purpose

lentiviral vector expressing sgRNA-4 targeting eIF3 binding site of RPS15A 5'UTR

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-4 targeting eIF3 binding site of RPS15A 5'UTR (RPS15A Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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PER2:Luc Plasmid

Plasmid

#206875

Purpose

Per2 promoter luciferase reporter

Depositor

Michelle Farkas

Article

Lin et al Integr Cancer Ther. 2019 Jan-Dec;18:1534

Insert

Period 2 (Per2 Mouse)

Use

Lentiviral and Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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BASU RaPID plasmid

Plasmid

#107250

Purpose

Expresses the BASU RaPID labeling protein

Depositor

Paul Khavari

Article

Ramanathan et al Nat Methods. 2018 Feb 5. pii: nmeth.4601

Insert

BASU

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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E.coli RaPID plasmid

Plasmid

#107251

Purpose

Expresses the E.coli RaPID labeling protein

Depositor

Paul Khavari

Article

Ramanathan et al Nat Methods. 2018 Feb 5. pii: nmeth.4601

Insert

E.coli BirA*

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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