Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Showing: 1 - 10 of 10 results

  2. pIAeBlue

    Plasmid
    #185891
    Purpose
    In vivo gene amplification (HamAmp) of AeBlue expression module at RPL25 locus
    Depositor
    Claudia Vickers
    Insert
    PRPL25(Arm 1)> Kl.LEU2>TKl.LEU2- TRPL25(Arm 3)- ARS305- PALD6>AeBlue>TPGK1- PBTS1> RPL25(partial; Arm2)
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    185891-plasmid-map-sequence-id-370863

    Enlarge

  3. pPAeBlueHPV16LR

    Plasmid
    #185893
    Purpose
    2-micron plasmid of AeBlue + HPV16 L1 expression module at RPL25 locus
    Depositor
    Claudia Vickers
    Insert
    PALD6>AeBlue>TPGK1- PSe.GAL2> HPV16-L1_C-6*H > TRPL41B
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    185893-plasmid-map-sequence-id-370866

    Enlarge

  4. pIAeBlueHPV16LR

    Plasmid
    #185894
    Purpose
    In vivo gene amplification (HamAmp) of AeBlue + HPV16 L1 expression module at RPL25 locus
    Depositor
    Claudia Vickers
    Insert
    PRPL25(Arm1)>Kl.LEU2>TKl.LEU2-TRPL25(Arm3)-ARS305-PALD6>EforRed>TPGK1-PSe.GAL2>HPV16-L1_C-6*H>TRPL41B-PBTS1>RPL25(partial;Arm2)
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    185894-plasmid-map-sequence-id-374392

    Enlarge

  6. pGLO-Blue

    Plasmid
    #220782
    Purpose
    Expresses a blue chromoprotein (visible light) with a his-tag and an inducible GFP (araBAD-promoter)
    Depositor
    Angela Leu
    Insert
    blue chromoprotein (aeBlue)
    Use
    Tags
    8xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    220782-plasmid-map-sequence-id-437912

    Enlarge

  7. pToxAmp12

    Plasmid
    #218286
    Purpose
    Multi-copy integration of heterologous genes (AeBlue and RelB) through co-transformation with ToxAmp (toxin-antitoxin-driven gene amplification) modules
    Depositor
    Claudia Vickers
    Article
    Evans et al bioRxiv 2024.01.30.578080
    Insert
    HO(-253, -1)-pCRT1>RelB>tPDC1-pTDH3>AeBlue>tsynth7-ARS712-HO(-731, -264)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    218286-plasmid-map-sequence-id-433688

    Enlarge

  8. pToxAmp21R

    Plasmid
    #218287
    Purpose
    A complete plasmid for ToxAmp (Toxin-antitoxin-driven gene amplification) for the expression of AeBlue
    Depositor
    Claudia Vickers
    Article
    Evans et al bioRxiv 2024.01.30.578080
    Insert
    HO(-955, -789)-LoxP>pKlLEU2>KlLEU2>PCRT1>RelB>tPDC1-pTDH3>AeBlue>tSYNth7-ARS712-HO(-731, -264)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    218287-plasmid-map-sequence-id-433689

    Enlarge

  9. MultiMate-PE3 HEK3

    Plasmid
    #206273
    Purpose
    Vector for the production of recombinant baculovirus using MultiMate assembly. All-in-one vector for PE3 HEK3 prime editing.
    Depositor
    Imre Berger
    Article
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    PE2 (synthetic), hU6 HEK3 PegRNA, hU6 HEK3 sgRNA, aeBlue (E. quadricolor), VSV-G, TagBFP
    Use
    CRISPR; Recombinant baculovirus production
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    206273-plasmid-map-sequence-id-406645

    Enlarge

  10. MultiMate-PE2 HEK3

    Plasmid
    #206274
    Purpose
    Vector for the production of recombinant baculovirus using MultiMate assembly. All-in-one vector for PE2 HEK3 prime editing. CRE-Acceptor in MultiBac system.
    Depositor
    Imre Berger
    Article
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    PE2 (synthetic), hU6 HEK3 PegRNA, hU6 HEK3 sgRNA, aeBlue (E. quadricolor), VSV-G, TagBFP
    Use
    CRISPR; Recombinant baculovirus production, cre-a…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    206274-plasmid-map-sequence-id-406650

    Enlarge

Showing: 1 - 10 of 10 results