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Plasmid#124947PurposeExpression of RFP-TEVproteaseDepositorInsertRFP-TEVprotease
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOpen-TEV protease
Plasmid#165509PurposeUsed for site-specific cleavage of proteins. TEV cleaves the protein consensus sequence EXXYXQ^S/GDepositorInsertTEV protease
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
EV-A71 2A protease; aliases: Enterovirus EV71 2A protease, 6xHis-SUMO-TEV tag, self cleaving
Plasmid#204810PurposeBacterial expresison of Enterovirus A71 2A proteaseDepositorInsertA71 2A protease
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 18, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28-MBP-super TEV protease
Plasmid#171782PurposeExpresses Maltose-binding protein-super TEV protease in bacterial cytoplasm. This protease version performs well in the absence of added reducing agent.DepositorInsertMBP-sTEV
UseTagsArg6 tag and Internal His6 tagExpressionBacterialMutationNo internal TEV cleavage site between the MBP and…PromoterT7Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
EV-D68 2A protease; 6xHis-GST-TEV, low expression levels
Plasmid#204804PurposeBacterial expresison of Enterovirus D68 2A proteaseDepositorInsertD68 2A protease
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
EV-A71 2A protease; 6xHis-MBP-TEV tag, self cleaving
Plasmid#204811PurposeBacterial expresison of Enterovirus A71 2A proteaseDepositorInsertA71 2A protease
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 16, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
EV-D68 2A protease; 6xHis-MBP-TEV tag, low expression levels
Plasmid#204803PurposeBacterial expresison of Enterovirus D68 2A proteaseDepositorInsertD68 2A protease
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBLO44.1_dProtease
Plasmid#121631PurposeCMV-intron_Pro_P2A-mTagBFP2_AmpR_ColE1DepositorInsertTEV protease
UseTagsExpressionMammalianMutationPromoterU6 and chicken beta-actin promoterAvailable sinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
Enterovirus A71 2A protease
Plasmid#204812PurposeBacterial expresison of Enterovirus A71 2A proteaseDepositorInsertA71 2A protease
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 20, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
MBP-TEVcs(ENLYFQ/G)-His6-uTEV1Δ(220-242)-R5
Plasmid#135455PurposeBacterial expression of uTEV1Δ proteaseDepositorInsertMBP-TEVcs(ENLYFQ/G)-His6-TEV1Δ(220-242)-R5
UseTagsMBP, His6ExpressionBacterialMutationS219V mutation improves stability, S153N mutation…Promotertac promoterAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
MBP-TEVcs(ENLYFQ/G)-His6-uTEV2Δ(220-242)-R5
Plasmid#135456PurposeBacterial expression of uTEV2Δ proteaseDepositorInsertMBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5
UseTagsMBP, His6ExpressionBacterialMutationS219V mutation improves stability, T30A/S153N mut…Promotertac promoterAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
MBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5
Plasmid#135454PurposeBacterial expression of TEVΔ proteaseDepositorInsertMBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5
UseTagsMBP, His6ExpressionBacterialMutationS219V mutation improves stability. Truncated form…Promotertac promoterAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
Tobacco Etch Virus NIa protease (GB0420)
Plasmid#160553PurposepUPD_NIaPro(TEV)DepositorInsertTobacco Etch Virus NIa protease
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
FRB_SCF2_LD6_CTEV
Plasmid#58879PurposeMESA split-TEV protease chain with Rapamycin FRB ectodomain 2 aa scaffold, 6 aa linker domain, C-terminal TEV proteaseDepositorInsertMESA split protease chain with FRB rapamycin-binding ectodomain and C terminal fragment of TEV protease
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
FKBP_SCF2_LD6_NTEV
Plasmid#58878PurposeMESA split-TEV protease chain with Rapamycin FKBP ectodomain, 2 aa scaffold, 6 aa linker domain, N-terminal TEV proteaseDepositorInsertMESA split protease chain with FKBP rapamycin-binding ectodomain and N terminal fragment of TEV protease
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-15b-TEV-C9R
Plasmid#118754PurposeTEV-C9R protease expression vector with enhanced expression, solubility and yield without affecting TEV's protease activityDepositorInsertTobacco Etch Virus protease (NEWENTRY Synthetic)
UseTagsC9R and His tagExpressionBacterialMutationPromoterT7Available sinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 iTEV
Plasmid#64277PurposeExpresses infrared fluorescent tobacco etch virus protease sensor (iTEV)DepositorInsertInfrared fluorescent tobacco etch virus protease sensor (iTEV)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-TEVP
Plasmid#179657PurposeTobacco Etch Viral ProteaseDepositorInsertTEVP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
TEVSH
Plasmid#125194PurposeHis-tagged TEV protease with solubility increasing mutationsDepositorInsertTEV_SH
UseTagsV5 His-tagExpressionBacterialMutationPromoterT7lacAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 TEV (full-length)
Plasmid#64276PurposeExpresses tobacco etch virus protease (TEV)DepositorInsertTobacco etch virus (TEV) protease
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only