Skip to main content
Addgene
Showing: 1 - 17 of 17 results

  7. ER2-mhYFP

    Plasmid
    #186528
    Purpose
    Localization and retention of monomeric hyperfolder YFP fluorescent protein in the ER; improved targeting efficiency
    Depositor
    Gregory Petsko
    Article
    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4
    Insert
    Monomeric hyperfolder YFP
    Use
    Tags
    Calreticulin and KDEL
    Expression
    Mammalian
    Mutation
    hfYFP-S147P/L195M/V206K
    Promoter
    CMV
    Available since
    Nov. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    186528-plasmid-map-sequence-id-373452

    Enlarge

  11. pCytERM-mhYFP

    Plasmid
    #186530
    Purpose
    Visualization of the ER in cells using monomeric hyperfolder YFP (compatible with OSER assay)
    Depositor
    Gregory Petsko
    Article
    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4
    Insert
    Monomeric hyperfolder YFP
    Use
    Tags
    Cytochrome p450 first 29 animo acids (Oryctolagus…
    Expression
    Mammalian
    Mutation
    hfYFP-S147P/L195M/V206K
    Promoter
    CMV
    Available since
    Nov. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    186530-plasmid-map-sequence-id-368240

    Enlarge

  13. ER2-hfYFP

    Plasmid
    #186518
    Purpose
    Localization and retention of hyperfolder YFP fluorescent protein in the ER; improved targeting efficiency
    Depositor
    Gregory Petsko
    Article
    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4
    Insert
    Hyperfolder YFP
    Use
    Tags
    Calreticulin and KDEL
    Expression
    Mammalian
    Mutation
    mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…
    Promoter
    CMV
    Available since
    Nov. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    186518-plasmid-map-sequence-id-368092

    Enlarge

  14. pCytERM-hfYFP

    Plasmid
    #186520
    Purpose
    Visualization of the ER in cells using hyperfolder YFP (compatible with OSER assay)
    Depositor
    Gregory Petsko
    Article
    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4
    Insert
    Hyperfolder YFP
    Use
    Tags
    Cytochrome p450 first 29 animo acids (Oryctolagus…
    Expression
    Mammalian
    Mutation
    mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…
    Promoter
    CMV
    Available since
    Nov. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    186520-plasmid-map-sequence-id-368218

    Enlarge

  17. pET28a-hfYFP-TEV-SAV

    Plasmid
    #191197
    Purpose
    Fusion of hfYFP and Streptavidin core domain separated by a TEV cleavage site for fluorescence-assisted protein purification
    Depositor
    Gregory Petsko
    Article
    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4
    Insert
    hfYFP & Streptavidin fusion
    Use
    Tags
    6xHis and Streptavidin core domain, amino acids 1…
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    191197-plasmid-map-sequence-id-376977

    Enlarge

Showing: 1 - 17 of 17 results