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Showing: 1 - 20 of 97 results

  1. pEDA5_GFPmut3_Y66H

    Plasmid
    #80085
    Purpose
    Positive control plasmid for Nicking Mutagenesis. Constitutively expresses a non-fluorescent variant of GFPmut3 (Y66H) that is mutated to fluorescent (H66Y) in the protocol.
    Depositor
    Timothy Whitehead
    Article
    Wrenbeck et al Nat Methods. 2016 Nov;13(11):928-930. do
    Insert
    GFPmut3_Y66H
    Use
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    changed Tyrosine 66 to Histidine
    Promoter
    proB
    Available since
    July 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    80085-plasmid-map-sequence-id-197645

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  2. R6K_tac_GFPmut3_ChInt

    Plasmid
    #187381
    Purpose
    chromosomal integration downstream of glmS gene via Tn7 system of a cassette containing IPTG-inducible GFPmut3
    Depositor
    Roi Avraham
    Insert
    GFPmut3
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    187381-plasmid-map-sequence-id-370413

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  3. R6K_tac_dGFPmut3_ChInt

    Plasmid
    #187388
    Purpose
    chromosomal integration downstream of glmS gene via Tn7 system of a cassette containing IPTG-inducible destabilized GFPmut3 (containing a AAV C-terminal tail)
    Depositor
    Roi Avraham
    Insert
    dGFPmut3_AAV
    Use
    Tags
    AAV
    Expression
    Bacterial
    Mutation
    C-terminal AAV tail
    Promoter
    Available since
    Aug. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    187388-plasmid-map-sequence-id-370402

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  6. pBAD_dGFPmut3-AAV

    Plasmid
    #187379
    Purpose
    arabinose-inducible expression of destabilized GFPmut3 (containing a AAV C-terminal tail)
    Depositor
    Roi Avraham
    Insert
    dGFPmut3_AAV
    Use
    Tags
    AAV
    Expression
    Bacterial
    Mutation
    C-terminal AAV tail
    Promoter
    Available since
    Aug. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    187379-plasmid-map-sequence-id-370386

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  7. pFPV25.1_dGFPmut3-LVA

    Plasmid
    #187378
    Purpose
    rpsM promoter driving the expression of destabilized GFPmut3 (containing a LVA C-terminal tail)
    Depositor
    Roi Avraham
    Insert
    dGFPmut3_LVA
    Use
    Tags
    LVA
    Expression
    Bacterial
    Mutation
    C-terminal LVA tail
    Promoter
    Available since
    Dec. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    187378-plasmid-map-sequence-id-380552

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  13. pELxG4

    Plasmid
    #172570
    Purpose
    Inducible gene expression, pET origin, LuxR activator, PLuxB promoter, GFPmut3, Carbenicillin Resistance Marker.
    Depositor
    Christopher Reisch
    Insert
    LuxR-GFPmut3
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLuxB
    Available since
    Dec. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    172570-plasmid-map-sequence-id-347356

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  14. pEVG4

    Plasmid
    #172571
    Purpose
    Inducible gene expression, pET origin, VanRAM repressor, PVanCC promoter, GFPmut3, Carbenicillin Resistance Marker.
    Depositor
    Christopher Reisch
    Insert
    VanRAM-GFPmut3
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PVanCC
    Available since
    Dec. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    172571-plasmid-map-sequence-id-347574

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Showing: 1 - 20 of 97 results