Addgene

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LRG2.1-CyOFP1

Plasmid

#124771

Purpose

3rd generation lentiviral plasmid to co-express a guide RNA and CyOFP1

Depositor

Jason Sheltzer

Article

Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pCS2_IRES2_CyOFP1_KCNQ2 var4

Plasmid

#173155

Purpose

For mammalian expression of KCNQ2 and creating variants

Depositor

Al George

Article

Simkin et al Elife. 2021 Feb 5;10. pii: 64434. doi: 1

Insert

KCNQ2 (KCNQ2 Human)

Use

Tags

IRES2-CyOFP1

Expression

Mammalian

Mutation

Promoter

Simian CMV IE94

Availability

Academic Institutions and Nonprofits only

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pKK-CyOFP1-TEV

Plasmid

#105782

Purpose

Expression of your protein of interest in fusion with orange fluorescent protein at the N-terminus (cleavable by TEV) (PMID: 27240196). CyOFP1 is useful for multi-channel imaging.

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

CyOFP1-TEV

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pKK-TEV-CyOFP1

Plasmid

#105799

Purpose

Expression of your protein of interest in fusion with orange fluorescent protein at the C-terminus (cleavable by TEV) (PMID: 27240196). CyOFP1 is useful for multi-channel imaging.

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

TEV-CyOFP1

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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SEP-Nanoluc-cyOFP1

Plasmid

#141082

Purpose

prototype of the bioluminescent reporter pHLuc, for detecting extracellular pH changes in tumor acidosis

Depositor

Jeak Ling Ding

Article

Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

Insert

SEP-Nanoluc-cyOFP1

Use

Lentiviral

Tags

Expression

Mutation

Deletion of amino acids DISGG from positions 673 …

Promoter

EF1α

Availability

Academic Institutions and Nonprofits only

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SEPLuc-E2A-cyOFP1

Plasmid

#141084

Purpose

precursor of the bioluminescent reporter pHLuc, for detecting extracellular pH changes in tumor acidosis

Depositor

Jeak Ling Ding

Article

Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

Insert

SEPLuc-E2A-cyOFP1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pIRES(A7Mut)_CyOFP1_KCNQ1

Plasmid

#173162

Purpose

For mammalian expression of KCNQ1 and creating variants

Depositor

Al George

Insert

KCNQ1 (KCNQ1 Human)

Use

Tags

IRES2(A7Mut)-CyOFP1

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
L2125-FRB-GPA-NLuc-CyOFP1

Plasmid

#222885

Purpose

Positive control receptor chain for BRET signal. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, full Nanoluciferase, 10 GS linker, CyOFP1.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FRB-GPA-20GS-NanoLuc-10GS-CyOFP1

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
L2127-FRB-GPA-114-20-CyOFP1

Plasmid

#222886

Purpose

Biosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 20 GS linker, CyOFP1 protein.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FRB-GPA-20GS-NanoLuc114-20GS-CyOFP1

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
L2128-FRB-GPA-114-10-CyOFP1

Plasmid

#222887

Purpose

Biosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 10 GS linker, CyOFP1 protein.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FRB-GPA-20GS-NanoLuc114-10GS-CyOFP1

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
L2129-FRB-GPA-114-5-CyOFP1

Plasmid

#222888

Purpose

Biosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 5 GS linker, CyOFP1 protein.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FRB-GPA-20GS-NanoLuc114-5GS-CyOFP1

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
L2130-FKBP-GPA-114-20-CyOFP1

Plasmid

#222889

Purpose

Biosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 20 GS linker, CyOFP1 protein.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FKBP-GPA-20GS-NanoLuc114-20GS-CyOFP1

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
L2131-FKBP-GPA-114-10-CyOFP1

Plasmid

#222890

Purpose

Biosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 10 GS linker, CyOFP1 protein.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FKBP-GPA-20GS-NanoLuc114-10GS-CyOFP1

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
L2132-FKBP-GPA-114-5-CyOFP1

Plasmid

#222891

Purpose

Biosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 5 GS linker, CyOFP1 protein.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FKBP-GPA-20GS-NanoLuc114-5GS-CyOFP1

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
L2092-GPA-FullBRET-Retrovirus-TD142

Plasmid

#222877

Purpose

Retroviral vector encoding positive control receptor chain for BRET signal. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, full Nanoluciferase, RH linker, CyOFP1.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FRB-GPA-NanoLuc-CyOFP1

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Promoter

MSCV LTR

Availability

Academic Institutions and Nonprofits only

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pEY108

Plasmid

#223455

Purpose

nsy-5 (prom4) fluorescent neural reporter driving nuclear CyOFP1 expression (refer to NeuroPAL paper for expression)

Depositor

Eviatar Yemini, Oliver Hobert

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

nsy-5(prom4)

Use

Tags

Expression

Worm

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pEY47

Plasmid

#191051

Purpose

gcy-21 fluorescent neural reporter driving nuclear CyOFP1 and mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Eviatar Yemini, Oliver Hobert

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

gcy-21 (gcy-21 Nematode)

Use

Tags

Expression

Worm

Mutation

None

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pEY48

Plasmid

#191052

Purpose

klp-6 fluorescent neural reporter driving nuclear mNeptune2.5 and CyOFP1 expression (refer to NeuroPAL paper for expression)

Depositor

Eviatar Yemini, Oliver Hobert

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

klp-6 (klp-6 Nematode)

Use

Tags

Expression

Worm

Mutation

None

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pEY49

Plasmid

#191053

Purpose

lim-6 fluorescent neural reporter driving nuclear mNeptune2.5 and CyOFP1 expression (refer to NeuroPAL paper for expression)

Depositor

Eviatar Yemini, Oliver Hobert

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

lim-6 (lim-6 Nematode)

Use

Tags

Expression

Worm

Mutation

None

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pEY51

Plasmid

#191054

Purpose

mec-3 fluorescent neural reporter driving nuclear CyOFP1 and mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Eviatar Yemini, Oliver Hobert

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

mec-3 (mec-3 Nematode)

Use

Tags

Expression

Worm

Mutation

None

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge