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Plasmid#227308PurposeExpresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of COX8A for knock-in.DepositorInsertsgRNA Targeting C-terminus of COX8A (COX8A Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU67Available sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSNAPf-Cox8A Control Plasmid
Plasmid#101129PurposeExpression of SNAP-Tagged Cox8A in Mammalian cellsDepositorInsertCox8A (COX8A Human)
UseTagsSNAP-tag (SNAPf)ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCLIPf-Cox8A Control Plasmid
Plasmid#101130PurposeExpression of CLIP-Tagged Cox8A in Mammalian cellsDepositorInsertCox8A (COX8A Human)
UseTagsCLIP-tag (CLIPf)ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCZGY1026 COX8aN::miniSOG in pENTR[1-2]
Plasmid#66752PurposeminiSOG fused to N terminal 29 aa of human Cox8a for targeting to the mitochondriaDepositorInsertminiSOG
UseTagsCOx8aN (N terminal 29 aa)ExpressionWormMutationPromoterAvailable sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-Solo-mStayGold-COX8A
Plasmid#227309PurposeDonor template for mStayGold insertion into the C-terminus of the COX8A locus. For mitochondria visualization. To be co-transfected with sgRNAplasmid px330-PITCh-COX8A (Addgene #227308)DepositorInsertCOX8A Homology Arms flanking a mStayGold Tag (COX8A Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_[Cox8a]x2_HaloTag9
Plasmid#175539PurposeExpression of HaloTag9 at the inner membrane of mitochondria in mammalian cellsDepositorInsertCox8a-HaloTag9
UseTagsExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_[Cox8a]x2_HaloTag7
Plasmid#175529PurposeExpression of HaloTag7 at the inner membrane of mitochondria in mammalian cellsDepositorInsertCox8a-HaloTag7
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_[Cox8a]x2_STf
Plasmid#182003PurposeCMV overexpression of SNAP-tag fast in the mitochondrial matrix of mammalian cell linesDepositorInsert[Cox8a]x2_STf
UseTagsExpressionMammalianMutationFast mutation on SNAP-tag E30RPromoterCMVAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcs2-MTS-cox8A-GFP-IRES-mCherry
Plasmid#226103PurposeStability reporter construct of the COX8A mitochondrial targeting sequence (MTS) for transient expression in mammalian cellsDepositorInsertCOX8A (COX8A Human)
UseTagsGFPExpressionMammalianMutationencodes only for mitochondrial targeting sequence…PromoterAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_Cox8a(4x)-SNAP-tag2-T2A-EGFP
Plasmid#226515PurposeMamalian cell expression of SNAP-tag2 in the mitochondrial matrix and cytosolic EGFPDepositorInsertCox8a(4x)-SNAP-tag2-T2A-EGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcs2-MTSmut-cox8A(XXXX)-GFP-IRES-mCherry
Plasmid#226104PurposeStability reporter construct of the COX8A mitochondrial targeting sequence (MTS) with mutations that impair recognition by SIFI for transient expression in mammalian cellsDepositorInsertCOX8A (COX8A Human)
UseTagsGFPExpressionMammalianMutationencodes only for mitochondrial targeting sequence…PromoterAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
N1-Mito-pHCountdown
Plasmid#182303Purposemammalian cell plasmid encoding pH-Countdown targeted to mitochondria via fusion to the COX8A presequenceDepositorInsertmitochondria targeted pH countdown
UseTagsCOX8A mitochondria targeting sequenceExpressionMammalianMutationPromoterCMVAvailable sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
mEos-A69V-Mito 7
Plasmid#57332PurposeLocalization: Mitochondria, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertMito (COX8A Human)
UseTagsmEos A69VExpressionMammalianMutationamino acids 1-29 of COX8A are presentPromoterCMVAvailable sinceJan. 16, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCDH-EF1-Mito-pHCountdown
Plasmid#182302Purposelentiviral plasmid encoding pH-Countdown targeted to mitochondria via fusion to the COX8A presequence with puromycin selectionDepositorInsertmito pH countdown
UseLentiviralTagsCOX8A mitochondria targeting sequenceExpressionMutationPromoterEF-1aAvailable sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
COX8-SuperNova
Plasmid#131856PurposeExpresses SuperNova fused to mitochrondrial protein COX8ADepositorInsertSuperNova
UseTagsCox8A and Myc tagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMito-Electra2
Plasmid#184927PurposeMitochondrial labeling with blue fluorescent protein Electra2 fused to partial COX8ADepositorInsertCOX8A-Electra2
UseTagsElectra2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMito-Electra1
Plasmid#179481PurposeMitochondrial labeling with blue fluorescent protein Electra1 fused to partial COX8ADepositorInsertCOX8A-Electra1
UseTagsElectra1ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-CCO-IRES-Puromycin
Plasmid#134861PurposeExpresses EGFP-tagged inner mitochondrial membrane markerDepositorInsertcytochrome c oxidase subunit VIII (COX8A Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
Mito-SBFP2
Plasmid#187964PurposeMammalian cell expression of a fluorescent SBFP2-tagged mitochondrial markerDepositorInsertMito (COX8A Human)
UseTagsSBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmTurquoise2-Mito
Plasmid#36208PurposeIn vivo visualization of the mitochondria (can be used for colocalization studies)DepositorInsertMTS (COX8A Human)
UseTagsmTurquoise2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only