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nls-NgAgo-GK

Plasmid

#78253

Purpose

Mammalian expression of NgAgo (Argonaute from N. gregoryi).

Depositor

Chunyu Han

Article

Gao et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

NgAgo

Use

Tags

a nuclear localization signal (NLS)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 2, 2016

Availability

Academic Institutions and Nonprofits only

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pET-6His-MBP-MpAgo

Plasmid

#79249

Purpose

This plasmid encodes the Argonaute protein from Marinitoga Piezophila and is codon optimized for E. coli expression

Depositor

Jennifer Doudna

Article

Kaya et al Proc Natl Acad Sci U S A. 2016 Mar 30. p

Insert

Marinitoga piezophila Argonaute

Use

CRISPR

Tags

6XHis-tag + MBP + PreScission Cleavage Site

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 1, 2016

Availability

Academic Institutions and Nonprofits only

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pFl Strep-sumo-TEV-hAgo2

Plasmid

#136235

Purpose

Expresses human Argonaute-2 in insect cells

Depositor

Leemor Joshua-Tor

Article

Elkayam et al Cell. 2012 Jul 6;150(1):100-10. doi: 10.

Insert

human Argonaute-2 (AGO2 Human)

Use

Tags

Strep Sumo

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

May 14, 2020

Availability

Academic Institutions and Nonprofits only

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pRSF-KpAGO207-1251

Plasmid

#117854

Purpose

Expressed KpAGO 207-1251 in E. coli under IPTG-inducible promotor

Depositor

Dinshaw Patel, Kotaro Nakanishi

Article

Nakanishi et al Nature. 2012 Jun 20;486(7403):368-74. do

Insert

Argonaute

Use

Tags

6xHis-SUMO encoded in vector

Expression

Bacterial

Mutation

missing first 206 amino acid residues

Promoter

T7

Available since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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Flag-nls-NgAgo-GK

Plasmid

#86435

Purpose

Expresses a Flag tag on the N-terminal

Depositor

Gaétan Burgio

Article

Khin et al PLoS One. 2017 Jun 13;12(6):e0178768. do

Insert

NgAgo

Use

Tags

Flag tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pFl Strep-sumo-TEV-hAgo2 D597N

Plasmid

#136236

Purpose

Expressing catalytically inactive human Argonaute-2 in insect cells

Depositor

Leemor Joshua-Tor

Article

Elkayam et al Nucleic Acids Res. 2017 Apr 7;45(6):3528

Insert

human Argonaute-2 (AGO2 Human)

Use

Tags

Sterp Sumo

Expression

Insect

Mutation

D597N

Promoter

polyhedrin

Available since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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EGFP-hAgo2

Plasmid

#21981

Depositor

Phil Sharp

Article

Leung et al Proc Natl Acad Sci U S A. 2006 Nov 28. 1

Insert

Argonaute 2 (AGO2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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pETSUMO-TtAgocatdead

Plasmid

#210322

Purpose

Bacterial expression of the His- and SUMO-tagged catalytically inactive Argonaute protein of Thermus thermophilus

Depositor

Phillip Zamore

Article

Jolly et al Cell. 2020 Sep 17;182(6):1545-1559.e18.

Insert

TtAgo (TT_P0026 Thermus thermophilus)

Use

Tags

6x His tag and SUMO tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmAGO1

Plasmid

#22768

Depositor

Elisa Izaurralde

Article

Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

Insert

Argonaut-1, variant A (AGO1 Fly)

Use

Drosophila expression

Tags

EGFP

Expression

Mutation

Promoter

Available since

Dec. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pAS2

Plasmid

#12389

Depositor

Nick Rhind

Article

Sigova et al Genes Dev. 2004 Oct 1. 18(19):2359-67.

Insert

green fluorescent protein

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 24, 2006

Availability

Academic Institutions and Nonprofits only

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pAS1

Plasmid

#12388

Depositor

Nick Rhind

Article

Sigova et al Genes Dev. 2004 Oct 1. 18(19):2359-67.

Insert

green fluorescent protein

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 24, 2006

Availability

Academic Institutions and Nonprofits only

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pCIneo-RLuc_C

Plasmid

#146090

Purpose

Mammalian Expression of Rluc

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

Rluc

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-N3-FLuc_C

Plasmid

#146092

Purpose

Mammalian Expression of Fluc

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

Fluc

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-RLuc-5BoxB_C

Plasmid

#146091

Purpose

Mammalian Expression of 5BoxB

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

5BoxB

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pET-GST

Plasmid

#42049

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

GST

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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pFLAG/HA-MOV10

Plasmid

#10976

Depositor

Thomas Tuschl

Article

Meister et al Curr Biol. 2005 Nov 9. ():.

Insert

MOV10 (MOV10 Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

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pmyc-MOV10

Plasmid

#10977

Depositor

Thomas Tuschl

Article

Meister et al Curr Biol. 2005 Nov 9. ():.

Insert

MOV10 (MOV10 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

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pmyc-GFP

Plasmid

#42028

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

EGFP

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 5, 2013

Availability

Academic Institutions and Nonprofits only

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pRS402-GPD-GFP(S65T)

Plasmid

#85190

Purpose

Integrating plasmid, GFP(S65T) under GPD promoter

Depositor

David Bartel

Article

Nakanishi et al Nature. 2012 Jun 20;486(7403):368-74. do

Insert

EGFP

Use

Integrating, shuttle vector

Tags

Expression

Yeast

Mutation

Promoter

GPD

Available since

Dec. 27, 2016

Availability

Academic Institutions and Nonprofits only

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pSUPER-DsRed

Plasmid

#42053

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

DsRed shRNA

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 1, 2013

Availability

Academic Institutions and Nonprofits only

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