These results may also be relevant.
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LCB7_crtYB
Plasmid#140232PurposeExpression of gene coding lycopene bifunctional β-cyclase for production beta-carotene from lycopeneDepositorInsertphytoene-beta carotene synthase
UseTagsExpressionBacterialMutationK307EPromoterpthrC3Available sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTarget12f1
Plasmid#171183PurposeConstitutive expression of sgRNA without donor DNADepositorInsertsgRNA scaffold in the CRISPR/Cas12f1 system
UseCRISPRTagsExpressionBacterialMutationPromoterJ23119(SpeI) promoterAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCas12f1
Plasmid#171182PurposeConstitutive expression of cas12f1, arabinose-inducible expression of lambda recombinase, and IPTG-inducible expression of sgRNA for curing of pTarget12f1DepositorInsertCas12f1
UseCRISPRTagsExpressionBacterialMutationPromotercas9 promoter from Streptococcus pyogenesAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only