1. pCFJ151 - ttTi5605_MCS

   Plasmid

   #19330

   Depositor

   Erik Jorgensen

   Article

   Christian et al

   Insert

   ttTi5605 targeting region
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Oct. 29, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

These results may also be relevant.

1. Lifeact-mCherry

   Plasmid

   #193300

   Purpose

   mammalian transient expression - expression of Lifeact-mCherry

   Depositor

   Klaus Hahn

   Article

   Herron et al Nat Commun. 2022 Jul 27;13(1):4363. doi:

   Insert

   Lifeact
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pX459_gRNA-AAVS1_hspCas9

   Plasmid

   #193309

   Purpose

   Cas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

   Insert

   Cas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   not applicable

   Promoter

   Available since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Mito-mTurq2-Apollo-NADP+

   Plasmid

   #193303

   Purpose

   Expresses mTurq2-Apollo-NADP+ sensor localized to the mitochondria

   Depositor

   Jonathan Rocheleau

   Article

   Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acss

   Insert

   G6PD (G6PD Human)
   

   Use

   Tags

   mTurqoise2 and mitochondrial targeting sequence

   Expression

   Mammalian

   Mutation

   S40A, R72Q, H201N, K205T

   Promoter

   T7

   Available since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Cyto-mTurq2-Apollo-NADP+

   Plasmid

   #193301

   Purpose

   Expresses mTurq2-Apollo-NADP+ sensor localized to the cytoplasm

   Depositor

   Jonathan Rocheleau

   Article

   Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acss

   Insert

   G6PD (G6PD Human)
   

   Use

   Tags

   mTurqoise2

   Expression

   Mammalian

   Mutation

   S40A, R72Q, H201N, K205T

   Promoter

   T7

   Available since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Nuc-mTurq2-Apollo-NADP+

   Plasmid

   #193302

   Purpose

   Expresses mTurq2-Apollo-NADP+ sensor localized to the nucleus

   Depositor

   Jonathan Rocheleau

   Article

   Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acss

   Insert

   G6PD (G6PD Human)
   

   Use

   Tags

   SV40 NLS and mTurqoise2

   Expression

   Mammalian

   Mutation

   S40A, R72Q, H201N, K205T

   Promoter

   T7

   Available since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Perox-mTurq2-Apollo-NADP+

   Plasmid

   #193304

   Purpose

   Expresses mTurq2-Apollo-NADP+ sensor localized to the peroxisome

   Depositor

   Jonathan Rocheleau

   Article

   Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acss

   Insert

   G6PD (G6PD Human)
   

   Use

   Tags

   mTurqoise2, peroxisomal targeting sequence

   Expression

   Mammalian

   Mutation

   S40A, R72Q, H201N, K205T

   Promoter

   T7

   Available since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pALS186 (ttTi5605-MCS loxP::C.br.unc-119(+)::loxP)

   Plasmid

   #129539

   Purpose

   modified pCFJ151 for C. elegans mosSCI at ttTi5605 or compatible sites; floxed C. br. unc-119(+) selection

   Depositor

   Arneet Saltzman

   Article

   Aram et al MicroPubl Biol. 2019 Aug 27;2019. doi: 1

   Type

   Empty backbone

   Use

   Cre/Lox; Mos1-mediated single copy insertion (mos…

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Oct. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHIT318

   Plasmid

   #204508

   Purpose

   rpl-21 promoter-driven C04F12.1 construct tagged with 2xHA for MosSCI integration at locus ttTi5605 on Chr II in nematode

   Depositor

   Yuji Kohara

   Article

   Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:

   Insert

   rpl-21p::2xHA::C04F12.1 (C. elegans)
   

   Use

   Tags

   2xHA

   Expression

   Worm

   Mutation

   Promoter

   rpl-21 (C. elegans)

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHIT246

   Plasmid

   #204507

   Purpose

   csr-1 construct tagged with 2xFLAG for MosSCI integration at locus ttTi5605 on Chr II in nematode

   Depositor

   Yuji Kohara

   Article

   Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:

   Insert

   2xFLAG::csr-1 (C. elegans) (csr-1 Nematode)
   

   Use

   Tags

   2xFLAG

   Expression

   Worm

   Mutation

   Promoter

   csr-1 (C. elegans)

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pALS297 (ttTi5605-MCS loxP::Prps-27::NeoR::unc-54-3ʹutr::loxP)

    Plasmid

    #129538

    Purpose

    modified pCFJ151 for C. elegans mosSCI at ttTi5605 or oxTi354 in otherwise wild-type background; floxed NeoR selection

    Depositor

    Arneet Saltzman

    Article

    Aram et al MicroPubl Biol. 2019 Aug 27;2019. doi: 1

    Type

    Empty backbone

    Use

    Cre/Lox; Mos1-mediated single copy insertion (mos…

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAG:H2B-EGFP

    Plasmid

    #32599

    Depositor

    Anna-Katerina Hadjantonakis, Virginia Papaioannou

    Article

    Hadjantonakis et al BMC Biotechnol. 2004 Dec 24;4:33.

    Insert

    H2B-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pUAST-attB-yki:V5-S168A

    Plasmid

    #85617

    Purpose

    Expresses V5 tagged mutant yki in Drosophila.

    Depositor

    Kenneth Irvine

    Article

    Oh et al Oncogene. 2009 Apr 30;28(17):1916-27. do

    Insert

    yki-V5-S168A
    

    Use

    Tags

    V5

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUAST-attB-yki:V5-S111A

    Plasmid

    #85619

    Purpose

    Expresses V5 tagged mutant yki in Drosophila.

    Depositor

    Kenneth Irvine

    Article

    Oh et al Oncogene. 2009 Apr 30;28(17):1916-27. do

    Insert

    yki-V5-S111A
    

    Use

    Tags

    V5

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUAST-attB-yki:V5-S250A

    Plasmid

    #85618

    Purpose

    Expresses V5 tagged mutant yki in Drosophila.

    Depositor

    Kenneth Irvine

    Article

    Oh et al Oncogene. 2009 Apr 30;28(17):1916-27. do

    Insert

    yki-V5-S250A
    

    Use

    Tags

    V5

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUAST-attB-yki:V5-S111A, S168A, S250A

    Plasmid

    #85620

    Purpose

    Expresses V5 tagged triple mutant yki in Drosophila.

    Depositor

    Kenneth Irvine

    Article

    Oh et al Oncogene. 2009 Apr 30;28(17):1916-27. do

    Insert

    yki-V5-S111A,S168,S250A
    

    Use

    Tags

    V5

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. C113S KVS-1-HA

    Plasmid

    #113070

    Purpose

    Expresses worm KVS-1 C113S in mammalian cells

    Depositor

    Federico Sesti

    Article

    Cai et al Nat Neurosci. 2009 May;12(5):611-7. doi:

    Insert

    C113S K+ voltage sensitive channel subunit 1 (kvs-1 Nematode)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    C113S

    Promoter

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. DeltaNIRD C95S KVS-1

    Plasmid

    #113068

    Purpose

    Expresses worm truncated KVS1.2 C113S in mammalian cells

    Depositor

    Federico Sesti

    Article

    Cai et al Nat Neurosci. 2009 May;12(5):611-7. doi:

    Insert

    C113S K+ voltage sensitive channel subunit 1.2 (kvs-1 Nematode)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C113S+first 40 AA deletion

    Promoter

    Available since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-Tag4A-MMP8 Wt

    Plasmid

    #29545

    Depositor

    Yardena Samuels

    Article

    Palavalli et al Nat Genet. 2009 May . 41(5):518-20.

    Insert

    Metalloproteinase protein 8 (MMP8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pCMV-Tag4A-MMP8 (P78S)

    Plasmid

    #29546

    Depositor

    Yardena Samuels

    Article

    Palavalli et al Nat Genet. 2009 May . 41(5):518-20.

    Insert

    Metalloproteinase protein 8 (MMP8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    P78S

    Promoter

    Available since

    July 22, 2011

    Availability

    Academic Institutions and Nonprofits only