1. pEcgRNA

   Plasmid

   #166581

   Purpose

   CRISPR-Cas9-assisted genome editing in Escherichia coli

   Depositor

   Sheng Yang

   Article

   Li et al Acta Biochim Biophys Sin (Shanghai). 202

   Insert

   ccdB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

These results may also be relevant.

1. pSADuet148

   Plasmid

   #121910

   Purpose

   Charge-introduced NpuDnaBΔ283ΔC39 intein

   Depositor

   Hideo Iwai

   Article

   Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

   Insert

   CI-NpuDnaBΔ290ΔC39 intein
   

   Use

   Tags

   His6-GB1

   Expression

   Bacterial

   Mutation

   Deletion of 290-residue endonuclease domain, I53K…

   Promoter

   T7

   Available since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHBDuet259

   Plasmid

   #121911

   Purpose

   NpuDnaBΔ283ΔC39 intein

   Depositor

   Hideo Iwai

   Article

   Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

   Insert

   NpuDnaBΔ283ΔC39 intein
   

   Use

   Tags

   His6-GB1

   Expression

   Bacterial

   Mutation

   Deletion of 283-residue endonuclease domain

   Promoter

   T7

   Available since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHBBAD113

   Plasmid

   #121912

   Purpose

   Charge-Introduced NpuDnaBC39 intein

   Depositor

   Hideo Iwai

   Article

   Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

   Insert

   CI-NpuDnaBC39 intein
   

   Use

   Tags

   GB1-His6

   Expression

   Bacterial

   Mutation

   S110K, I111K

   Promoter

   Para

   Available since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHBDuet116

   Plasmid

   #121915

   Purpose

   NpuDnaBΔ290ΔC39 intein

   Depositor

   Hideo Iwai

   Article

   Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

   Insert

   Oth-NpuDnaBΔ290ΔC39 intein
   

   Use

   Tags

   His6-GB1

   Expression

   Bacterial

   Mutation

   Deletion of 290-residue endonuclease domain, I53K…

   Promoter

   T7

   Available since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHBBAD168

   Plasmid

   #121916

   Purpose

   Oth-NpuDnaBC39 intein

   Depositor

   Hideo Iwai

   Article

   Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

   Insert

   Oth-NpuDnaBC39 intein
   

   Use

   Tags

   GB1-His6

   Expression

   Bacterial

   Mutation

   I111K, E116K

   Promoter

   Para

   Available since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHBDuet139

   Plasmid

   #121913

   Purpose

   Charge-Introduced NpuDnaB mini-intein

   Depositor

   Hideo Iwai

   Article

   Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

   Insert

   CI-NpuDnaBΔ290 intein
   

   Use

   Tags

   GB1 and His6-GB1

   Expression

   Bacterial

   Mutation

   Deletion of 290-residue endonuclease domain, I53K…

   Promoter

   T7

   Available since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHBDuet140

   Plasmid

   #121914

   Purpose

   Orthogonal NpuDnaB mini-intein intein

   Depositor

   Hideo Iwai

   Article

   Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

   Insert

   Oth-NpuDnaBΔ290 intein
   

   Use

   Tags

   GB1 and His6-GB1

   Expression

   Bacterial

   Mutation

   Deletion of 290-residue endonuclease domain, I53K…

   Promoter

   T7

   Available since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge