1. psPAX2

   Plasmid

   #12260

   Purpose

   2nd generation lentiviral packaging plasmid. Can be used with 2nd or 3rd generation lentiviral vectors and envelope expressing plasmid (Addgene#12259)

   Depositor

   Didier Trono

   Type

   Empty backbone

   Use

   Lentiviral; Packaging

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

These results may also be relevant.

1. pBT120b

   Plasmid

   #122604

   Purpose

   BE PACE selection, express gIII, luxAB and degron-GTCCA guide RNA

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   gIII, luxAB, guide RNA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBT44c

   Plasmid

   #122600

   Purpose

   BE PACE selection, express C-intein-Cas9-ugi fusion

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   intein-dCas9-ugi fusion
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBT277

   Plasmid

   #122608

   Purpose

   express evoCDA1-BE4max in mammalian cells

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   evoCDA1-BE4max
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   F23S A123V I195F

   Promoter

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBT137-splitC

   Plasmid

   #122605

   Purpose

   clone M13 phage by SapI Golden Gate

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   M13 bacteriophage genes
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBT100.106b

   Plasmid

   #122601

   Purpose

   clone APOBEC1 phage by SapI Golden Gate

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   APOBEC1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBT137-splitD

   Plasmid

   #122606

   Purpose

   clone M13 phage by SapI Golden Gate

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   M13 bacteriophage genes
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBT276

   Plasmid

   #122607

   Purpose

   express CDA1-BE4max in mammalian cells

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   CDA1-BE4max
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBT279

   Plasmid

   #122609

   Purpose

   express FERNY-BE4max in mammalian cells

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   FERNY-BE4max
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBT100.110

   Plasmid

   #122602

   Purpose

   clone CDA1 phage by SapI Golden Gate

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   CDA1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBT100.136c

    Plasmid

    #122603

    Purpose

    clone FERNY phage by SapI Golden Gate

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    FERNY
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJRH-1180 U6-reci Gag-pol v2

    Plasmid

    #201915

    Purpose

    Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    Gag-pol
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUC19-E3CY56

    Plasmid

    #103075

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    E3CY56(Cas9 coding gene from Aminomonas paucivorans DSM 12260)
    

    Use

    CRISPR; Cloning vector

    Tags

    Expression

    Mutation

    human codon-optimized

    Promoter

    Available since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSG06-pLac-NRI

    Plasmid

    #102463

    Purpose

    Expresses NRI protein in E. coli. The expression is controlled by a pLac promoter.

    Depositor

    Richard Murray

    Article

    Guo et al bioRxiv 122606

    Insert

    NRI (ntrC )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLac

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSG10-pGlnA-mCherry

    Plasmid

    #102467

    Purpose

    Expresses mCherry fluorescent protein in E. coli. The expression is controlled by a pGlnA promoter.

    Depositor

    Richard Murray

    Article

    Guo et al bioRxiv 122606

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pGlnA

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSG05-pGlnA-deGFP

    Plasmid

    #102462

    Purpose

    Expresses deGFP fluorescent protein in E. coli. The expression is controlled by a pGlnA promoter.

    Depositor

    Richard Murray

    Article

    Guo et al bioRxiv 122606

    Insert

    deGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pGlnA

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSG07-pLac-NRII_L16R

    Plasmid

    #102464

    Purpose

    Expresses NRII protein with L16R mutation in E. coli. The expression is controlled by a pLac promoter.

    Depositor

    Richard Murray

    Article

    Guo et al bioRxiv 122606

    Insert

    NRII (ntrB )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Leucine 16 to Arginine

    Promoter

    pLac

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSG08-pLac-NRII_H139N

    Plasmid

    #102465

    Purpose

    Expresses NRII protein with H139N mutation in E. coli. The expression is controlled by a pLac promoter.

    Depositor

    Richard Murray

    Article

    Guo et al bioRxiv 122606

    Insert

    NRII (ntrB )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Histidine 139 to Asparagine

    Promoter

    pLac

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSG09-OR2-OR1-Pr-sigma54

    Plasmid

    #102466

    Purpose

    Expresses sigma 54 protein in E. coli. The expression is controlled by a OR2-OR1-Pr promoter.

    Depositor

    Richard Murray

    Article

    Guo et al bioRxiv 122606

    Insert

    sigma 54 (rpoN )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    OR2-OR1-Pr

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. KHBD00519

    Plasmid

    #39620

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG33221 (CG33221 Fly)
    

    Use

    Gateway donr vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Sept. 19, 2012

    Availability

    Academic Institutions and Nonprofits only