1. pSico

   Plasmid

   #11578

   Depositor

   Tyler Jacks

   Article

   Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 19, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

These results may also be relevant.

1. LV-Sox10MCS5-GFP

   Plasmid

   #115783

   Purpose

   This plasmid encodes for Sox10-MCS5-GFP reporter. Cell carrying this construct expresses green fluorescence under the control of SOX-MCS5 enhancer conjugated with cfos basal promoter.

   Depositor

   Fraser Sim

   Article

   Pol et al Exp Neurol. 2013 Sep;247:694-702. doi: 1

   Insert

   mouse derived SOX10 Multiple Species Conserved enhancer element 5 conjugated with cfos basal promoter
   

   Use

   Lentiviral

   Tags

   cfos basal promoter conjugated MCS5 promoter fuse…

   Expression

   Mutation

   Promoter

   SOX10 Multiple Species Conserved enhancer element…

   Available since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. LV-Sox10MCS5-GFP-EF1a-mCherry

   Plasmid

   #115782

   Purpose

   Sox10-MCS5-GFP and a constitutive EF1α-mCherry reporter plasmid. Construct generates constitutive red fluorescence and basal promoter cfos conjugated SOXMCS5 enhancer driven GFP expression in a cell.

   Depositor

   Fraser Sim

   Article

   Pol et al Exp Neurol. 2013 Sep;247:694-702. doi: 1

   Inserts

   mouse derived SOX10 Multiple Species Conserved enhancer element 5 conjugated with cfos basal promoter
   EF1alpha promoter driven constitutive mCherry
   

   Use

   Lentiviral

   Tags

   EF1α promoter fused to mCherry and cfos promoter …

   Expression

   Mutation

   Promoter

   SOX10 Multiple Species Conserved enhancer element…

   Available since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA 3.3 HA-hTSPAN12

   Plasmid

   #115784

   Purpose

   Expresses human TSPAN12 with N-terminal HA-tag in mammalian cells. Contains multiple silent mutations that introduce restriction sites.

   Depositor

   Harald Junge

   Article

   Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

   Insert

   TSPAN12 (TSPAN12 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   multiple silent mutations introduce restriction s…

   Promoter

   CMV

   Available since

   Oct. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.3 ss-3xFLAG-hLRP5 (aa 32-1615)

   Plasmid

   #115788

   Purpose

   Expresses human LRP5 with N-terminal 3xflag tag in mammalian cells, contains artificial signal sequence upstream of tag.

   Depositor

   Harald Junge

   Article

   Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

   Insert

   LRP5 (LRP5 Human)
   

   Use

   Tags

   3xflag

   Expression

   Mammalian

   Mutation

   contains silent mutation that creates an internal…

   Promoter

   CMV

   Available since

   Oct. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA 3.3 EGFP

   Plasmid

   #115786

   Purpose

   Expresses EGFP in mammalian cells

   Depositor

   Harald Junge

   Article

   Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.3 ss-FLAG-AP-hNDP (aa 25-133)

   Plasmid

   #115789

   Purpose

   Expresses and secretes human Norrin with N-terminal flag and alkaline phosphatase tags, contains artificial signal sequence upstream of tags.

   Depositor

   Harald Junge

   Article

   Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

   Insert

   NDP (NDP Human)
   

   Use

   Tags

   alkaline phosphatase and flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. Pinducer20-BSD-scFv-sfGFP-DNMT3A1

   Plasmid

   #115780

   Purpose

   targeted DNA methylation tool

   Depositor

   Margaret Goodell

   Article

   Su et al Genome Biol. 2018 Aug 10;19(1):108. doi:

   Insert

   scFv-sfGFP-DNMT3A1 (DNMT3A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA 3.3 ss-V5-hFZD4 (aa 37-537)

   Plasmid

   #115787

   Purpose

   Expresses human FZD4 with N-terminal V5-tag in mammalian cells, contains artificial signal sequence upstream of tag.

   Depositor

   Harald Junge

   Article

   Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

   Insert

   FZD4 (FZD4 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA 3.3 HA-TSPAN12^11-LEL

   Plasmid

   #115785

   Purpose

   Expresses a chimera in mammalian cells: the large extracellular loop of TSPAN12 is replaced with TSPAN11 sequence

   Depositor

   Harald Junge

   Article

   Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

   Insert

   TSPAN12/11 chimera (TSPAN11, TSPAN12 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   large extracellular loop of TSPAN12 replaced with…

   Promoter

   CMV

   Available since

   Oct. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSICO-Flpo

    Plasmid

    #24969

    Depositor

    Tyler Jacks

    Article

    Young et al Proc Natl Acad Sci U S A. 2010 Jun 1. 10

    Insert

    Flp recombinase
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSICO-eGFP-TNPO3

    Plasmid

    #167590

    Purpose

    Expresses human TNPO3 fused to eGFP

    Depositor

    Zandrea Ambrose

    Article

    Zhong et al mBio. 2021 Mar 23;12(2). pii: mBio.03142

    Insert

    eGFP-TNPO3 (TNPO3 Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mutation

    WT

    Promoter

    Available since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSICO-CPSF6-eGFP

    Plasmid

    #167585

    Purpose

    Expresses human CPSF6 fused to eGFP

    Depositor

    Zandrea Ambrose

    Article

    Zhong et al mBio. 2021 Mar 23;12(2). pii: mBio.03142

    Insert

    CPSF6-eGFP (CPSF6 Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mutation

    WT

    Promoter

    Available since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSICO-CPSF6-mNeonGreen

    Plasmid

    #167587

    Purpose

    Expresses human CPSF6 fused to mNeonGreen

    Depositor

    Zandrea Ambrose

    Article

    Zhong et al mBio. 2021 Mar 23;12(2). pii: mBio.03142

    Insert

    CPSF6-mNeonGreen (CPSF6 Human)
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mutation

    WT

    Promoter

    Available since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSICO-CPSF6-358-eGFP

    Plasmid

    #110693

    Purpose

    Expresses a truncated form of CPSF6 (CPSF6-358 originally described in Lee et al. Cell Host Microbe 2010) fused with eGFP

    Depositor

    Zandrea Ambrose

    Article

    Ning et al J Virol. 2018 Apr 11. pii: JVI.00368-18.

    Insert

    CPSF6-eGFP (CPSF6 )
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Truncation of CPSF6 isoform 2 at amino acid 358

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSICO-CPSF6-358-iRFP670

    Plasmid

    #110694

    Purpose

    Expresses a truncated form of CPSF6 (CPSF6-358 originally described in Lee et al. Cell Host Microbe 2010) fused with iRFP670

    Depositor

    Zandrea Ambrose

    Article

    Ning et al J Virol. 2018 Apr 11. pii: JVI.00368-18.

    Insert

    CPSF6-iRFP670 (CPSF6 Human)
    

    Use

    Lentiviral

    Tags

    iRFP670

    Expression

    Mutation

    Truncation of CPSF6 isoform at amino acid 358

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSICO-CPSF6-iRFP670

    Plasmid

    #167586

    Purpose

    Expresses human CPSF6 fused to iRFP

    Depositor

    Zandrea Ambrose

    Article

    Zhong et al mBio. 2021 Mar 23;12(2). pii: mBio.03142

    Insert

    CPSF6-iRFP670 (CPSF6 Human)
    

    Use

    Lentiviral

    Tags

    iRFP670

    Expression

    Mutation

    WT

    Promoter

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET30-ARH3-His-Sumo-HA

    Plasmid

    #111578

    Purpose

    Expresses ARH3 (amino acids 14-363) in E. coli

    Depositor

    Thomas Muir

    Article

    Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

    Insert

    ARH3 (ADPRS Human)
    

    Use

    Tags

    6xHis-Sumo and HA

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-13

    Promoter

    T7

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEGFP-N1-PARP1

    Plasmid

    #211578

    Purpose

    CMV driven PARP1-eGFP expressing construct with IRES-Neomycin selection

    Depositor

    Chris Lord

    Article

    Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

    Insert

    PARP1 (PARP1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSICO-CPSF6-4Glu-iRFP670

    Plasmid

    #167588

    Purpose

    Expresses a mutant form of CPSF6 (CPSF6-4Glu described in Jang et al. Nucleic Acids Res 2019) fused to iRFP670

    Depositor

    Zandrea Ambrose

    Article

    Zhong et al mBio. 2021 Mar 23;12(2). pii: mBio.03142

    Insert

    CPSF6-4Glu-iRFP670 (CPSF6 Human)
    

    Use

    Lentiviral

    Tags

    iRFP670

    Expression

    Mutation

    K547E, R549E, R559E, R561E

    Promoter

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge