1. pU6-pegRNA-GG-acceptor

   Plasmid

   #132777

   Purpose

   Prime editing in mammalian cells

   Depositor

   David Liu

   Article

   Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

   Insert

   Exchangeable cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   See manuscript

   Promoter

   U6

   Available since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

These results may also be relevant.

1. NOLC1 atgRNA pDY2250

   Plasmid

   #219860

   Purpose

   attachment site pegRNA for human NOLC1

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

   Insert

   NOLC1 atgRNA (NOLC1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. NOLC1 nicking guide pDY2251

   Plasmid

   #219861

   Purpose

   Nicking sgRNA for NOLC1

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

   Insert

   NOLC1 nicking sgRNA (NOLC1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDY2260 hNOLC1 atgRNA Paired Guide 2

   Plasmid

   #220990

   Purpose

   hNOLC1 atgRNA

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

   Insert

   NOLC1 atgRNA (NOLC1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDY2261 hACTB atgRNA Paired Guide 1

   Plasmid

   #220991

   Purpose

   hACTB atgRNA

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

   Insert

   ACTB atgRNA (ACTB Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDY2262 hACTB atgRNA Paired Guide 2

   Plasmid

   #220992

   Purpose

   hACTB atgRNA

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

   Insert

   ACTB atgRNA (ACTB Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDY2259 hNOLC1 atgRNA Paired Guide 1

   Plasmid

   #220989

   Purpose

   hNOLC1 atgRNA

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

   Insert

   NOLC1 atgRNA (NOLC1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pU6-pegRNA-SNCA-A53T

   Plasmid

   #181738

   Purpose

   Transiently expressing a pegRNA to introduce SNCA-A53T mutation in human cells

   Depositor

   Dirk Hockemeyer

   Article

   Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

   Insert

   Prime editing pegRNA for SNCA-A53T (SNCA Synthetic)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pU6-pegRNA-SNCA-A30P

   Plasmid

   #180016

   Purpose

   Transiently expressing a pegRNA to introduce SNCA-A30P mutation in human cells

   Depositor

   Dirk Hockemeyer

   Article

   Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

   Insert

   Prime editing pegRNA for SNCA-A30P (SNCA Synthetic)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pU6-pegRNA-LRRK2-G2019S-3a

   Plasmid

   #180432

   Purpose

   Transiently expressing a pegRNA to introduce LRRK2-G2019S mutation in human cells

   Depositor

   Frank Soldner

   Article

   Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

   Insert

   ncRNA targeting LRRK2-G2019S (LRRK2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   LRRK2-G2019S

   Promoter

   U6

   Available since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pU6-pegRNA-HEK3-CTTins-px330-scaffold

    Plasmid

    #180017

    Purpose

    Transiently expressing a pegRNA to introduce HEK3 CTT insertion in human cells. It has a sgRNA scaffold from px330.

    Depositor

    Dirk Hockemeyer

    Article

    Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

    Insert

    Prime editing pegRNA for HEK3-CTTins, with px330 scaffold (EPHA8 Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pU6-pegRNA-LRRK2-G2019S-3b

    Plasmid

    #180433

    Purpose

    Transiently expressing a pegRNA to introduce LRRK2-G2019S mutation in human cells

    Depositor

    Frank Soldner

    Article

    Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

    Insert

    ncRNA targeting LRRK2-G2019S (LRRK2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    LRRK2-G2019S

    Promoter

    U6

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pU6-pegRNA-LRRK2-G2019S-3c

    Plasmid

    #180434

    Purpose

    Transiently expressing a pegRNA to introduce LRRK2-G2019S mutation in human cells

    Depositor

    Frank Soldner

    Article

    Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

    Insert

    ncRNA targeting LRRK2-G2019S (LRRK2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    LRRK2-G2019S

    Promoter

    U6

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pU6-pegRNA-LRRK2-G2019S-3d

    Plasmid

    #180435

    Purpose

    Transiently expressing a pegRNA to introduce LRRK2-G2019S mutation in human cells

    Depositor

    Frank Soldner

    Article

    Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

    Insert

    ncRNA targeting LRRK2-G2019S (LRRK2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    LRRK2-G2019S

    Promoter

    U6

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge