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Plasmid#128213Purposeimproved sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU3 promoter module (pFH38)DepositorInsertimproved sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU3 promoter module (pFH38)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
peSpCas9-J23109
Plasmid#113152PurposePlasmid constitutively expressing eSpCas9 proteinDepositorInserteSpCas9
UseTagsExpressionBacterialMutationK810A & K1003A & R1060APromoterAvailable sinceDec. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.SFFV.sfGFP-RepEntry.iPAC
Plasmid#100893PurposeLentiviral CRISPR-Cas9 ReporterDepositorInsertssfGFP
PAC
UseTagsExpressionMutationPromoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFTK086
Plasmid#171358PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertP-ANtRNA[Arg21]-sgRNA-dummy-Esp3I, Pol-III sgRNA-plug-in transcription unit
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
BC1364-mouse U6-sgH2B
Plasmid#164043PurposeU6-driven sgRNA targeting H2BDepositorInsertH2B targeting sgRNA
UseCRISPRTagsExpressionMutationPromotermouse U6Available sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
peSpdCas9-J23109
Plasmid#113153PurposePlasmid constitutively expressing eSpdCas9 proteinDepositorInserteSpdCas9
UseTagsExpressionBacterialMutationD10A & K810A & H840A & K1003A & R…PromoterAvailable sinceDec. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX330.LoxP
Plasmid#66586PurposesgRNA for LSLDepositorInsertLoxP sgRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSP571
Plasmid#139498PurposeCRISPR-Cas9 plasmid to generate double strand break in STL1 locus in S. cerevisiae. Expresses both Cas9 and STL1 sgRNADepositorInsertpGPD Cas9 / sgRNA (STL162)
UseTagsExpressionYeastMutationWTPromoterpGPDAvailable sinceJune 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFH71
Plasmid#128214Purposeimproved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003)DepositorInsertimproved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-hsg(M)
Plasmid#138262PurposeSubcloning and expression of mCherry-targeting sgRNA M for use in Traffic Light reporter systemDepositorInsertsgRNA M
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBluescript_VEGFA-TS3_SpCas9
Plasmid#107328PurposeU6 driven SpCas9 sgRNA expression for VEGFA site 3DepositorInsertVEGFA-TS3 sgRNA
UseCRISPRTagsExpressionMutationPromoterU6Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBluescript_VEGFA-TS3_NmCas9
Plasmid#107329PurposeU6 driven NmCas9 sgRNA expression for VEGFA site 3DepositorInsertVEGFA-TS3 sgRNA
UseCRISPRTagsExpressionMutationPromoterU6Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCCM937
Plasmid#58982PurposeExpresses avr-15 sgRNA in C.elegans.DepositorInsertavr-15 sgRNA
UseCRISPRTagsExpressionWormMutationPromoterU6Available sinceJuly 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCCM936
Plasmid#58981PurposeExpresses avr-14 sgRNA in C.elegans.DepositorInsertavr-14 sgRNA
UseCRISPRTagsExpressionWormMutationPromoterU6Available sinceJuly 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pX330-PCNT
Plasmid#227284PurposeExpresses SpCas9 and a sgRNA targeting the N-terminus of PCNT for knock-in.DepositorArticleInsertsgRNA Targeting N-terminus of PCNT (PCNT Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX330-PITCh-PXN
Plasmid#227315PurposeExpresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of PXN for knock-in.DepositorArticleInsertsgRNA Targeting C-terminus of PXN (PXN Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX458-PLK4
Plasmid#227310PurposeExpresses SpCas9 and a sgRNA targeting the C-terminus of PLK4 for knock-in.DepositorArticleInsertsgRNA Targeting C-terminus of PLK4 (PLK4 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTAG4E
Plasmid#176868PurposeFirst sgRNA in a tRNA separated array of sgRNAsDepositorInsertsgRNA_4E
UseCRISPRTagsExpressionMutationWTPromoterAvailable sinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTAG5
Plasmid#176869PurposeFirst sgRNA in a tRNA separated array of sgRNAsDepositorInsertsgRNA_5
UseCRISPRTagsExpressionMutationWTPromoterAvailable sinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTAG6E
Plasmid#176870PurposeFirst sgRNA in a tRNA separated array of sgRNAsDepositorInsertsgRNA_6E
UseCRISPRTagsExpressionMutationWTPromoterAvailable sinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only