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pUPD2+p35SoTMV

Plasmid

#170876

Purpose

Phytobrick - p35SoTMV

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

p35SoTMV

Use

Tags

Expression

Plant

Mutation

214C>T and 229C>T to remove BbsI restrictio…

Promoter

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM1761

Plasmid

#105381

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

promoter AtDMC1 (At3G22880; 3000bp)

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI/ BpiI restriction sites removed

Promoter

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pOVC1_NcVVD-LOV_X

Plasmid

#131176

Purpose

Expresses NcVVD-LOV (Neurosporra crassa) followed by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

NcVVD-LOV

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pOVC1_AtPHYB-S_X

Plasmid

#131183

Purpose

Expresses AtPHYB-S (Arabidopsis thaliana) followed by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

AtPHYB-S

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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TTVTFLPTPSLILQT_epitope

Plasmid

#165021

Purpose

Expression of CMV UL44 (38-52) fused to CD74

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

CMV UL44 (38-52)

Use

Lentiviral

Tags

CD74 at N-term; cathepsin S cleavage site (GRWHTV…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pOVC1_ScPH1-S_X

Plasmid

#131184

Purpose

Expresses ScPH1-S (Synechosystis PCC6803) followed by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

ScPH1-S

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pOVC1_RsLP-LOV_X

Plasmid

#131177

Purpose

Expresses RsLP-LOV (Rhodobacter sphaeroides) followed by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

RsLP-LOV

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAS1_4x7SKPylT_EF1_Ub-BsmBI

Plasmid

#162801

Purpose

STELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient expression

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-BsmBI

Use

Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pJOG331

Plasmid

#105408

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

6xHA tag fused to Twin-Strep tag

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI/ BpiI restriction sites removed

Promoter

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTEI071

Plasmid

#105376

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

promotor CAB3 (At1g29910; 1000bp)

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI/ BpiI restriction sites removed

Promoter

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET11a-TAX-L10-STEPtag

Plasmid

#159375

Purpose

Expresses STEPtag fused to the C-terminus of inactive T. aurantiacus xylanase 10A, as a mock protein of interest.

Depositor

Roberto Chica

Article

Eason et al ACS Synth Biol. 2020 Nov 20;9(11):2955-2

Insert

TAX-L10-STEPtag

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pET11a-STEPtag-L10-TAX

Plasmid

#159374

Purpose

Expresses STEPtag fused to the N-terminus of inactive T. aurantiacus xylanase 10A, as a mock protein of interest.

Depositor

Roberto Chica

Article

Eason et al ACS Synth Biol. 2020 Nov 20;9(11):2955-2

Insert

STEPtag-L10-TAX

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCK032

Plasmid

#105412

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

Hormone-binding domain (glucocorticoid receptor)

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI/ BpiI restriction sites removed

Promoter

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pYPQ290-D570A

Plasmid

#129673

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AacCas12b

Use

CRISPR; Gateway compatible aaccas12b-d570a entry …

Tags

Expression

Plant

Mutation

D570A; AacCas12b is rice codon optimized

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pOVC1_X_VfAU1-LOV

Plasmid

#131185

Purpose

Expresses VfAU1-LOV domain (Vaucheria frigida) preceded by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

VfAU1-LOV

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pOVC1_VfAU1-LOV_X

Plasmid

#131174

Purpose

Expresses VfAU1-LOV domain (Vaucheria frigida) followed by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

VfAU1-LOV

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pOVC1_X_MxCarH-CBD

Plasmid

#131189

Purpose

Expresses MxCarH-CBD (Myxococcus xanthus) preceded by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

MxCarH-CBD

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pOVC1_X_TtCarH-CBD

Plasmid

#131190

Purpose

Expresses TtCarH-CBD (Thermus termophilus) preceded by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

TtCarH-CBD

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Flag YFP hNKCC1 L717C C723S C724V (NT531)

Plasmid

#50896

Purpose

Expresses human NKCC1 L717C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

Depositor

Biff Forbush

Insert

human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)

Use

Tags

3x Flag and YFP

Expression

Mammalian

Mutation

L717C C723S C724V in hNKCC1 in NT17

Promoter

CMV

Available since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pJOG301

Plasmid

#105358

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

promotor DD45/EC1.2 (At2g21740;1000bp)

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI/ BpiI restriction sites removed

Promoter

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge